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Exogenous Sucrose Affected AtKEA1 and AtKEA2 to Regulate Root Growth of Seedling in Arabidopsis thaliana Sheng ZHENG, Haixia GAO, Min SU, Shanghuan LU, Tengguo ZHANG, Guofan WU Bulletin of Botanical Research    2023, 43 (4): 562-571.   DOI: 10.7525/j.issn.1673-5102.2023.04.009 Abstract （324）   HTML （11）    PDF（pc） （5300KB）（1826）       Knowledge map    Save To explore the function roles of K+-efflux-antiporters KEA1 and KEA2 in Arabidopsis thaliana， wild type and kea1kea2 knock-down mutants of Arabidopsis thaliana were used as materials to investigate the effects of KEA1 and KEA2 on plant growth and development. Phenotypic analysis， propidium iodide staining to observe the structure of roots， high performance liquid chromatography to determine endogenous sugar content， transcriptome sequencing and qRT-PCR to analyze the relative expression levels of related genes， histochemical staining to detect the distribution of superoxide anion in leaves were performed respectively. The results showed that the root length of the kea1kea2 mutant was significantly shorter than that of the wild-type Col-0 plant in the absence of sucrose. Further observation showed that compared with the wild-type， the root meristem zone of kea1kea2 mutant was shorter， the endogenous sucrose content was decreased， and more O2·- was distributed in the leaves of the kea1kea2 mutant. However，when 30 g·L-1 sucrose was exogenously added， there was no significant difference in root length between the wild-type and kea1kea2 mutant. Transcriptome analysis revealed that the expression of many key genes involved in sucrose signals and root growth were repressed in the kea1kea2 mutant. In summary， the results suggested that sucrose might affect AtKEA1and AtKEA2 to regulate root growth in Arabidopsis seedlings. Table and Figures | Reference | Related Articles | Metrics

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Effects of Three Kinds of Agrobacterium and Different Transformation Conditions on the Transient Expression of GFP in Nicotiana benthamiana Yuejing Zhang, Ying Li, Juanjuan Wang, Hailong Pang, Lingyun Jia, Hanqing Feng Bulletin of Botanical Research    2022, 42 (1): 121-129.   DOI: 10.7525/j.issn.1673-5102.2022.01.013 Abstract （4589）   HTML （137）    PDF（pc） （4568KB）（1879）       Knowledge map    Save By using Nicotiana benthamiana as plant material， the influence of the different Agrobacterium strains（LBA4404， EHA105， GV3101）， bacterial concentration and infection time on GFP（as the reporter gene） the fluorescence transient expression after transformation were analyzed respectively. The results showed that the expression of GFP with different Agrobacterium strains were various in the optimal concentration and time for transient： the highest transient expression efficiency with LBA4404 strain was achieved when the OD600 of bacterial suspension was 0.8； while the highest transient expression efficiency with EHA105 and GV3101 strains was achieved when the OD600 of the bacterial suspension reached 0.6. The level of transient expression by strain LBA4404 was the highest at 2 days later Agrobacterium infiltration while the level of transient expression by strains EHA105 and GV3101 was the highest at 4 days later Agrobacterium infiltration. The comparative analysis among these different strains showed that the transient expression efficiency by using LBA4404 strain was the highest. The above results indicated that Agrobacterium strains and transformation conditions such as bacterial concentration and infection time were important factors that affected the transient expression efficiency. Table and Figures | Reference | Related Articles | Metrics

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Analysis of Non-Cellulosic Monosaccharide Composition of Poplar Cell Wall by HPAEC-PAD Xiaoling JIN, Huimin WU, Chaofeng YANG, Jin ZHANG, Mengzhu LU, Wei ZENG Bulletin of Botanical Research    2023, 43 (5): 787-793.   DOI: 10.7525/j.issn.1673-5102.2023.05.015 Abstract （744）   HTML （8）    PDF（pc） （835KB）（335）       Knowledge map    Save To establish an ion exchange chromatography-pulsed amperometric detection （HPAEC-PAD） method for non-cellulosic monosaccharides in poplar（Populus spp.） cell walls， the 9 main cell wall monosaccharides could be base-line separated in one run by optimizing the separation conditions. Dionex CarbiPacTM PA100 （4 mm×250 mm） with sodium hydroxide analytical column was used for gradient elution at flow rate is 1.0 mL·min-1 and the column temperature of 30 ℃ was used for the determination of monosaccharide fraction in the cell wall of different poplar species. The results showed that the 9 monosaccharides had a good linear relationship in the linear range of 0.5-150.0 mg·L-1， the correlation coefficient R2 was 0.999 0-0.999 3， the reproducibility（RSD） of the method was 1.09%-3.96%， and the recovery rate by standard addition was 91.32%-109.25%. The detection limit was 1.57×10-3-1.41×10-2 mg·L-1. Galacturonic acid was the highest composition followed by xylose， and the glucuronic acid content was the lowest monosaccharide. The method developed in this study was widely applicable and simple， and could be used for the detection of monosaccharides in the cell walls of poplar and other species. Table and Figures | Reference | Related Articles | Metrics

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Construction and Identification of TRV-mediated VIGS Transformation System of Iris sanguinea Guiling LIU, Nuo XU, Gongfa SHI, Ling WANG Bulletin of Botanical Research    2024, 44 (1): 132-138.   DOI: 10.7525/j.issn.1673-5102.2024.01.015 Abstract （112）   HTML （3）    PDF（pc） （1718KB）（201）       Knowledge map    Save To analyze gene function in plants lacking stable genetic transformation system， virus-induced gene silencing（VIGS） was needed， and Iris sanguinea， a monocotyledon， was selected as materials. The specific fragment of IsPDS gene was isolated and the VIGS recombinant vector pTRV2-IsPDS was constructed and leaves were infected by injection. The results showed that the most effective infection was achieved by injecting its leaf veins with syringes when the OD600 values of the resuspension were adjusted to 0.8-1.0 after the those of pTRV1 and pTRV2-IsPDS were adjusted to 1.8-2.0. The experiment was conducted when outdoors temperature was 15-20 ℃ from 6 p.m. to 8 p.m.， and a 1 mL syringe needle pricking the outer epidermis of I. sanguinea leaves and 1 mL of heavy suspension slowly injected along parallel veins into its vascular bundles. A clear albino phenotype might appear after about 14 days. TRV1 and TRV2 virus vectors were detected in the plants with phenotypic changes and in the no-load group. The expression of IsPDS in the albino plants was significantly lower than that in the no-load group and the control group. With the concentration of agrobacterium tumefaciens carrying virus vector increased in the preparation of infection solution， the infection efficiency of the whole experiment was improved， and no shading was needed after inoculation. Table and Figures | Reference | Related Articles | Metrics

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Cloning and Functional Analysis of EgrWAT1 Gene in Eucalyptus grandis Haonan ZHANG, Shanshan CHEN, Jianmin XU, Ping LUO, Xiaoping WANG, Zhiru XU, Chunjie FAN Bulletin of Botanical Research    2023, 43 (4): 601-611.   DOI: 10.7525/j.issn.1673-5102.2023.04.013 Abstract （1544）   HTML （3）    PDF（pc） （2728KB）（470）       Knowledge map    Save In order to explore the role of WALLS ARE THIN（WAT1） in wood formation and response to stress in woody plants， bioinformatics tools was used for analysis， and quantitative Real-time PCR（qRT-PCR） was used to investigate the expression patterns of EgrWAT1L and EgrWAT1S in different tissues， internodes and in response to different stresses， and the gene EgrWAT1S and its other transcript EgrWAT1L were cloned from Eucalyptus grandis. The results showed that the EgrWAT1S was highly expressed in phloem， while EgrWAT1L was mainly expressed in roots， and the expression patterns were significantly different under methyl jasmonate（MeJA） and salicylic acid（SA） treatment， salt stress， phosphorus（P） and boron（B） deficiency， and even opposite under MeJA and SA. These results suggested that EgrWAT1L might affect EgrWAT1S expression through transcriptional regulation and further protein translation in response to hormone and stress treatments. The studies provided a basis for further elucidate the function in the growth and development of EgrWAT1 gene and also provided a possibility for future molecular breeding of Eucalyptus. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on the Regulatory Mechanism of Plant Root Hair Development Yuping QIU, Yichuan WANG, Hongwei GUO Bulletin of Botanical Research    2023, 43 (3): 321-332.   DOI: 10.7525/j.issn.1673-5102.2023.03.001 Abstract （1512）   HTML （42）    PDF（pc） （2312KB）（440）       Knowledge map    Save Root hairs are tubular extensions of plant root epidermal cells that play vital roles in soil fixation， water and inorganic salts absorption， and information exchange between plant roots and outside world. The developmental processes of root hair are highly plastic and might easily be affected by various plant hormones and environmental factors. Due to the structural and functional characteristics of root hair， it is often used as model to study apical growth and fate determination of plant cells. Therefore， the regulatory mechanism of root hair development has been a hot topic in the field of botany. The review summarized the research progress in the field of root hair development in the past two decades. Table and Figures | Reference | Related Articles | Metrics

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Selection of Excellent Families and Evaluation of Selection Method for Pulpwood Half-sibling Families of Betula platyphylla Binghua CHEN, Jie ZHANG, Guifeng LIU, Siting LI, Yuanke GAO, Huiyu LI, Tianfang LI Bulletin of Botanical Research    2023, 43 (5): 690-699.   DOI: 10.7525/j.issn.1673-5102.2023.05.006 Abstract （103）   HTML （6）    PDF（pc） （907KB）（183）       Knowledge map    Save To provide the theoretical basis for genetic improvement of Betula platyphylla， the genetic variation of the growth and wood characters of B. platyphylla half-sibling families were studied， and the excellent pulp families were selected， and 32 seven-year-old half-sibling families were measured respectively， and the growth and wood characters of each family were analyzed by ANOVA， genetic parameters were calculated and general combining ability was estimated， multiple selection methods were used to select excellent families and evaluate excellent parents. The results showed that there were significant differences （P<0.01） in growth and wood characters among families， and the family heritability was 0.71-1.00， the heritability per plant was 2.0-3.0， the coefficient of variation ranged from 5.88% to 49.84%. There was a significantly and strongly positive correlation among DBH， tree height and volume（r：0.70-0.97）， and fiber length to width ratio had a significantly and strongly positive correlation with the fiber length（r：0.69）， while it had a significantly and strongly negative correlation with the fiber width（r：-0.76）. The general combining ability was used to evaluate the excellent parents， and the general combining ability ranges of volume， basic density and fiber length were -0.001 1-0.001 0， -0.04-0.07 and -112.09-77.36， respectively. The maternal parents of A13， A12， A21 and A24 were selected as excellent parents， and three different selection methods， including Brekin multi-trait comprehensive analysis method， principal component analysis method and breeding value method， were used to select the excellent family. After comparing the advantages and disadvantages of the three methods， five excellent pulp families were selected， and the average genetic gain of volume and cellulose reached 12.97% and 3.40%， respectively. A12， A17， A23， A19， A1 were selected as the excellent pulpwood families， which provided material for further breeding of improved varieties of fast- growing B. platyphylla. Table and Figures | Reference | Related Articles | Metrics

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Ultrasonic-Assisted Enzymatic Extraction of Polysaccharides from Schisandra chinensis and the Effects on Anti-Oxidative Stress in Cells Hanlin ZHU, Heng ZHAO, Bowen ZHAI, Maoyu ZHANG, Yujie FU Bulletin of Botanical Research    2023, 43 (4): 631-640.   DOI: 10.7525/j.issn.1673-5102.2023.04.016 Abstract （1858）   HTML （13）    PDF（pc） （3235KB）（1664）       Knowledge map    Save The polysaccharides of Schisandra chinensis were extracted by ultrasonic-assisted cellulase enzymolysis from the dried fruit of S. chinensis as raw material， and the extraction process and anti-oxidative stress activity of S. chinensis polysaccharides were studied. Using polysaccharide yield as index， single factor experiment and Box-Behnken response surface test were used to investigate the effects of enzyme concentration， ultrasonic power， extraction time and solid-liquid ratio on polysaccharide yield of S. chinensis， and the ultrasonic-assisted enzymatic extraction process was optimized. A lipopolysaccharide（LPS） induced oxidative stress damage model of HepG2 cells was established， and the levels of malondialdehyde（MDA）， superoxide dismutase（SOD） and reactive oxygen species（ROS） were used as indicators， and the anti-oxidative stress ability of S. chinensis polysaccharides was investigated. The results showed that the optimal extraction conditions as followed： the enzyme concentration was 1 420 U·g-1， the ultrasonic power was 500 W， the extraction time was 46 min， the solid-liquid ratio was 1 g∶32 mL. Under these conditions， the yield of polysaccharides was 22.25%. Moreover， the extracted S. chinensis polysaccharides might improve the SOD level in the LPS-induced HepG2 cells， but reduce the MDA accumulation and ROS fluorescence intensity in the cells， and effectively relieve the LPS-induced oxidative stress in HepG2 cells. The extraction conditions of polysaccharides from S. chinensis were optimized， and it was found that the extracted polysaccharides had good antioxidant stress ability. Table and Figures | Reference | Related Articles | Metrics

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Effect of Expression of Aeβ- AS gene from Aralia elata on the Content of Saponins in Nicotiana tabacum L. Qingqing HUO, Yuxin XIA, Jiale LI, Wei HAN, Shuya ZHANG, Zhe ZHANG, Meiling XIA, Wenhua GUO, Xiangling YOU Bulletin of Botanical Research    2023, 43 (5): 779-786.   DOI: 10.7525/j.issn.1673-5102.2023.05.014 Abstract （1408）   HTML （3）    PDF（pc） （1728KB）（213）       Knowledge map    Save To investigate the effect of β-amyrin synthase gene（Aeβ-AS） on triterpenoid saponin synthesis of Aralia elata， the Aeβ-AS gene was cloned and genetically transformed into tobacco. The expression difference of Aeβ-AS gene in different parts of transgenic tobacco was analyzed， and the expression levels of Aeβ-AS gene， upstream and downstream key enzyme genes and total triterpene content were detected respectively. The results showed that the plant overexpression vector PROKⅡ-Aeβ-AS was successfully constructed and transferred into wild type tobacco， and seven transgenic lines were obtained and expressed at the mRNA level， and the expression level in leaves was higher than that in roots and stems. In transgenic tobacco， the expression levels of Aeβ-AS gene and its upstream and downstream key enzyme genes were higher than those of wild type， and the relative expression levels of NtFPS and NtSS genes of strain L21 were the highest， and the relative expression levels of NtSE and Aeβ-AS genes of strain L30 were the highest. Compared with wild-type tobacco， the total triterpene content of GM tobacco was significantly increased（1.1-1.6 times）. The results showed that synthesis of Aeβ-AS gene and heterologous transformation in tobacco could significantly increase the total triterpene content in transgenic tobacco. Table and Figures | Reference | Related Articles | Metrics

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Identification of Knockout of BRI1 Mutant in Arabidopsis Mediated by CRISPR/Cas9 Guo-Fan WU, Hong-Bin CHENG, Yu-Jun WU, Juan SHEN, Wang-Ze WU Bulletin of Botanical Research    2021, 41 (3): 362-371.   DOI: 10.7525/j.issn.1673-5102.2021.03.006 Abstract （1711）   HTML （102）    PDF（pc） （5071KB）（1049）       Knowledge map    Save CRISPR/Cas9 gene editing technology was used to specificity edit the BRI1 of Arabidopsis thaliana to creation new BRI1 mutants. We analyzed the sequence of BRI1 in the obtained transgenic plants， the results showed that as one new BRI1 mutant due to the insertion of new bases， the normal BRI1 protein is terminated prematurely. This mutant have the similar phenotype as bri1-710， and both mutants show impaired response to BL treatment compared with wild-type. Those results suggested that the N-terminal of BRI1 may play a role in the BR signaling pathway. Therefore， this study could provide a reliable reference for further studies on the BRI1 function of A. thaliana and other homologous species. Table and Figures | Reference | Related Articles | Metrics

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Fine Root Biomass and Root Length Density of Pinus tabulaeformis and Cupressus chengiana Plantations in the Arid Valleys of the Upper Minjiang River Juan XIA, Xudong SUN, Na WANG, Rui LI, Juan CHEN, Guoqiang GAO Bulletin of Botanical Research    2024, 44 (2): 259-266.   DOI: 10.7525/j.issn.1673-5102.2024.02.011 Abstract （29）   HTML （3）    PDF（pc） （1032KB）（81）       Knowledge map    Save To investigate the vertical distribution of root biomass（diameter≤2 mm） and root length density of Pinus tabulaeformis and Cupressus chengiana plantations in the arid valleys of the upper Minjiang River， and to analyze the carbon allocation strategy of fine root system in different soil layers， and to provide reference for vegetation restoration in the arid valleys of the upper Minjiang River. P. tabulaeformis and C. chengiana plantations were sampled by soil corer method， and the root biomass and root length density of absorptive roots（first to third order） and transport roots（≥fourth order） in different depth（h）（0 cm<h≤15 cm and 15 cm<h≤30 cm） were measured， as well as the proportions biomass and length density of absorptive roots to the total fine roots. The results showed that： the absorptive root biomass and root length density of P. tabulaeformis and C. chengiana were significantly higher in 0 cm<h≤15 cm than those in 15 cm<h≤30 cm， and the transport root biomass and root length density were not significantly different between soil layers； the proportions biomass and length density of absorptive roots to the total fine roots in 0 cm<h≤15 cm were significantly higher than that in 15 cm<h≤30 cm（P<0.05）； the proportions biomass and length density of absorptive roots to the total fine roots in 0 cm<h≤15 cm and 15 cm<h≤30 cm of C. chengiana were significantly higher than those of P. tabulaeformis（P<0.05）. These findings suggested that more carbon was allocated to the absorptive roots in the surface soil layers with the highest nutrient availability in P. tabulaeformis and C. chengiana root system. Table and Figures | Reference | Related Articles | Metrics

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Recent Advances on Plant Root Thermomorphogenesis Wei LIU, Ziqiang ZHU Bulletin of Botanical Research    2024, 44 (1): 1-7.   DOI: 10.7525/j.issn.1673-5102.2024.01.001 Abstract （131）   HTML （24）    PDF（pc） （1194KB）（196）       Knowledge map    Save Plant development is highly plastic. As ambient temperature increased， plant stems and petioles elongated， and promoted leaf surface cooling， the process known as thermomorphogenesis. High temperatures also triggered root elongation， which named as root thermomorphogenesis. There were many studies on the regulation of plant shoot thermomorphogenesis， but few investigations on the regulatory mechanisms of plant root thermomorphogenesis. In this review， we summarized the recent progresses in the field of plant root thermomorphogenesis， and proposed future research directions. Table and Figures | Reference | Related Articles | Metrics

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MORPHOLOGY, TAXONOMY AND DISTRIBUTION OF THE GENUS RHAMNUS L.(RHAMNACEAE) IN THE NORTHEASTERN CHINA Li Shi-you, Ning Zhu-hua Bulletin of Botanical Research    1988, 8 (2): 71-114.   Abstract （3193）      PDF（pc） （4333KB）（519）       Knowledge map    Save The morphological variation of the trunks, branches, leaves, buds, flowers, fruits and seeds of the species are analysed on the basis of thedata of field observations and specimen studies, and some confuscd spe-cies were also surveyed in the field periodically. The results are as fol-lows:1. Habit. Tlie species are all decidous shrubs or small trees, andthe heights of the plants vary with the habitat. It is of limited taxonomic significance in the species. 2. Branches. The species may be opposite or alternate, and often with spines. The branch color is rather variable within and among species andvaries with the light conditions. It has no taxonomic significance in thisgenus. 3. Leaves. A few species are opposite or nearly opposite such as Rh.diamantiaca, but most species are alternate. Leaves vary in shape withinand among species in this genus, but the basic leaf shape is an importantcharacter, which can be used to distinguish most species. The value ofleaf length/width has some significance in taxonomy of this genus.Most species are easily distinguished by their leaves alone, using features such as basic leaf shape, value of leaf length/width, vein number and surface of leaf. 4. Buds. The species are different in bud shape and color. Most specieshave no terminal buds except Rh. arguta and Rh. davurica var. davurica. 5. Flowers. The flowers of Rhamnus are relatively small and inconspi-cous, and are characteristic of the genus. The species in the northeasternChina are all the same dioecious, greenish, 4-nerous, in axillary clusters. The pedicel length, heirs occuring calyx sad pedicel, are of some signifi-cance in taxonomy of infraspecific taxa. 6. Fruits and Seeds. As reproductive organs, they are of special significance in taxonomy of Rhamnus. In this paper, we employ the characters.of fruit and seed to distinguish the taxa usually at the specific and sup-raspecific levels. Rh. arguta and Rh. cathartica are with 3-4(2) seeds perfruit, the other species with 2 seeds per fruit. Endocarp character is im-portant in the classification of subsection. In Subsect. Rhamnus, endocarps are membranceus an can not be separated easily from the seeds, but in Subsect. Virgatiformes Grub., they are os-seus and can be separated easily from the seeds. The seed surface features(rough or glossy and the color) are the same. The open part length ofthe seed furrow differs significantly among most species, and it is an im-portant criterion of classification. Pedicel varies in length from 0.2cm to2.0cm among and within species, and it has statistical significance in ta-xonomy. 7. Seedlings. The germination features of the seed and the morphological chafacteristics ot the cotyledons of 6 species and 2 varieties have been studied. All species are epigeal. The species can be divided into twogroups in cotyledon morphology:the cotyledon of Rh. arguta is elliptic, and it is similar to Hovenia dulcis, the stomata of the cotyledon is ellip-tic, the guard cell is narrow, there are few chloroplasts in the guard cell:In the other species, e cotyledons are round, the stomatas of the cotyledons are round, the guard cells are round, and there are many chlotaplasts in the guard cells. There is a close correlation among various features, in morphology, cytology, isoenzyme and pollen (Li and Ning, in press). It seems that, in the course of morphological evolution in Rhamnus, the divergence of other features also occurred. According to results above, the anthors divide the genus Rhamnus in thenortheastern China into 2 Snbsections, 4 Series, 10 species and 3 varieties:Rhamnus L. Sect. Rhamnus Subsect. 1, Rhamnus Ser. 1, Utiles Grub. 1, Rh. davurica Pall. a. var. davurica b. var. nipponica Makino 2. Rh. utilis Decne var. hypochrysa (Schn.) Rehd.Ser. 2, Rhamnus 3, Rh. cathartica L.Subsect. 2, Virgatiformes Grub.Ser. 3, Japonicae Grub. 4, Rh. koraiensis Schn.Ser. 4, Parvifoliae Grub. 5, Rh. parvifolia Bunge 6, Rh. dalianensis S. Y. Li et Z. H. Ning 7, Rh. bungeana J. Vass 8, Rh. diamantiaca Nakai. 9, Rh.globosa Bunge a. var. globosa b. var. mey'ri (Schn). Y. Li et Z.H.Ning 10, Rh. yoshinoi Maklno 11, Rh. arguta Maxim. Rh. dalianensis is a new species which was found in Dalian, Liaoning Province. Rh. meyeri Schn. has been combined as a variety under Rh. globosa Bunge, Rh. yoshinoi Makino and Rh. diamanliaca Nakai are placed in Ser. Parvifoliae Grub, from Ser. Japonicae Grub.. The boundaries of some species have been defined. The key to understanding the species boundaries in the genus lies in their floral morphology. The identification of the species in Rhamnus is based largely on basic leaf shape. vein number, hairs in leaf And pedicel, the open part length of the seed furrow. In superspecific taxa, the size of leaf, seed number per fruit, endocarp character and seed surface feature are used to distinguish them. The species of Rhamnus in the northeastern China can be divided into three geographically defined groupsf Eurychoric Plants, such as Rh. davurica, Rh. davurica var. nippontca, Rh. yoshinctf Rh diamantiaca and Rh. parvifolia, distribute widely in the northeasrtern China, and occuring in the other regions of China or other countries;Stenochoric Plants, such as Rh. arguta, Rh. koraiensist Rh. bungeana and Rh. globosa var. meyeri, distribute in the northeastern China, and they also occur in the other regions of China;Endemic Plant, Rh. dalianensis, is restricted to the Liaodong Peninsula.With the increase of the air temperature, the number of species graincreases from north to south ia the northeastern China, To the north of latitude 50°N, there is only one species, Rh. davuncat but in Liaodong Peninsula (the southern part of northeastern China), there are 7 species and 2 varieties. Liacdoug Peninsula is the center of taxoccmic diversity for Rhamtius in the northeastern Cbina. From the eastern part (humid, subhumid climate), to the western part (arid, semiarid climate), the number of species gradually decreases ficm 5 species ard 2 varieties to 2 species. Related Articles | Metrics

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The Chloroplast Division Protein PARC6 Affected the Growth of Cotyledon and Leaf in Arabidopsis thaliana Zhuanzhuan JIANG, Li GONG, Yaling SONG Bulletin of Botanical Research    2023, 43 (5): 700-710.   DOI: 10.7525/j.issn.1673-5102.2023.05.007 Abstract （79）   HTML （6）    PDF（pc） （5572KB）（345）       Knowledge map    Save Abnormal chloroplast division homozygous mutant strain parc6 and albinistic cotyledon homozygous mutant strain sco2 were identified at the DNA， RNA and cellular levels， while the effects of chloroplast abnormal division on the growth of cotyledon and leaf were investigated by adding different concentrations of sucrose to the medium and taking sl2， the double mutant with leaf abnormal division and cotyledon albino as the control. The results showed that the cotyledon of sco2 mutant was albino， while the leaf grew normally. Meanwhile， the cotyledon growth and viability of the parc6 mutant were significantly lower than the wild-type， which was equivalent to the sco2 mutant， however， the double mutant sl2 with cotyledon albinism and leaf abnormal division was severely hindered. The leaf growth and viability of the parc6 mutant were also significantly lower than that of the wild type， but recovered compared to the cotyledon. The inhibited cotyledon growth phenotype of the parc6 mutant and sco2 mutant could be confirmed by the addition of carbon source to the medium， but chloroplast fluorescence parameters of sco2 were different from the wild type. The results of BN-PAGE combined with SDS-PAGE showed that the high-level structure of the photosystem of the cotyledon and leaf were normal， indicating that chloroplasts of parc6 failed to produce enough energy to affect the growth of cotyledon and leaf. The phylogenetic analysis showed that PARC6 and SCO2 coevolved， suggesting a connection between cotyledon development and chloroplast division. Our results indicated that chloroplast size was closely related to plant growth， especially the development of cotyledon， which provided a new perspective for revealing chloroplast function. Table and Figures | Reference | Related Articles | Metrics

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The Species Diversity of the Floras of Daxing’an Mountain, Xiaoxing’an Mountain and Ecotone ZHANG Ling;YUAN Xiao-Ying*;ZHANG Dong-Lai Bulletin of Botanical Research    2007, 27 (3): 356-360.   DOI: 10.7525/j.issn.1673-5102.2007.03.020 Abstract （1882）      PDF（pc） （150KB）（765）       Knowledge map    Save Typical plots were established in Daxing’an mountain,Xiaoxing’an mountain,and ecotone of the Notheast of China Plant species composition and vertical structure of different distribution regions were studied in Daxing’an Mountain, Xiaoxing’an Mountain and ecotone using species richness, Simpson diversity index, Shannon-Wiener index and evenness index. Meanwhile, the biodiversity and evenness index of the same community in different regions and different life-forms were compared and analysed.The results indicated:There is significant characteristic of transition between Daxing’an Mountain and Xiaoxing’an Mountain. Theopencedrymion dominates Xiaoxing’an Mountain and ecotone,conifer-conifer forest dominates Daxing’an Mountain;The species diversity indexes of three regions are Xiaoxing’an Mountain＞econtone＞Daxing’an Mountain;The species diversity of different life-forms in different plant regions are herb layer＞shrub layer＞tree layer. Species diversity was significant different in tree layer, but not found in shrubs layer and herb layer. Reference | Related Articles | Metrics

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STUDY ON DIVERSITY OF VASCULAR PLANT OF WETLAND IN SHANXI SHANGGUAN Tie-liang, ZHANG Feng, ZHANG Long-sheng, SONG Bo-wei Bulletin of Botanical Research    2000, 20 (3): 275-281.   Abstract （411）      PDF（pc） （269KB）（486）       Knowledge map    Save It is first published that there are 686 species of vascular plants of wetland in Shanxi Province, which belong to 308 genera and 83 families. Among them there are about 8 families, 10 genera, 18 species in Pteridophyta. 56 families, 223genera, 504 species in Dicotyledoneae and 19 families, 75 genera, 164 species in Monocotyledoneae. There are various geographical elements among genera, in which the temperate elements take dominant position and make account for 76.9% except cosmopolitan genera. This phenomenon is the result of the broad latitudes and diversified habitats in Shanxi. Cosmopolitan elements has 74 genera. The elements accounts 24% in total genera, and is main components or key genera in wetland vegetation. Endemic of China is scarce in the flora. Related Articles | Metrics

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Rediscovery of Ulota germana(Mont.) Mitt.(Orthotrichaceae) In New Zealand WANG Qing-Hua, JIA Yu Bulletin of Botanical Research    2018, 38 (1): 1-6.   DOI: 10.7525/j.issn.1673-5102.2018.01.001 Abstract （2775）      PDF（pc） （2421KB）（4457）       Knowledge map    Save Ulota germana(Mont.) Mitt., formly as U.rufula(Mitt.) A.Jaeger, was recorded from only Marlborough District of South Island in New Zealand and it has not been refound for more than 60 years. A new locality is added here: Nelson, Denniston, near parking area at top of Denniston Incline. The plants from New Zealand agree well with those from South America except some variation in the size of leaves, perichaetial leaves and spores. This new specimen is well described, especially the opercula and calyptra not seen in previous works, the distribution is mapped and its distinction from other close species is discussed here. Ulota luteola(Hook.f. & Wilson) Wijk & Margad., formly as U.pygmaeothecia(Müll. Hal.) Kindb. is excluded from the moss flora of New Zealand. Reference | Related Articles | Metrics

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A New Species of Iris L.(Iridaceae) From Yunnan,China DONG Xiao-Dong;LI Ji-Hong Bulletin of Botanical Research    2008, 28 (2): 136-137.   DOI: 10.7525/j.issn.1673-5102.2008.02.003 Abstract （3008）      PDF（pc） （127KB）（959）       Knowledge map    Save Iris habanesis X. D. Dong is described and illustrated as a new species. This species is distinguished from Iris decora Wall. in ground controlled approach, single scape, no branch and having crista tailpiece. Also, it differs from Iris collettii Hook. f. by having scape 15-20 centimeters long in fluorescence. Reference | Related Articles | Metrics

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Study on Germination Characteristics and Germination Rate Enhancement of Saposhnikovia divaricata Seeds MENG Xiang-Cai;SUN Hui;WANG Xi-Jun* Bulletin of Botanical Research    2008, 28 (5): 627-631.   DOI: 10.7525/j.issn.1673-5102.2008.05.026 Abstract （2780）      PDF（pc） （171KB）（1487）       Knowledge map    Save The germination rate and germination vigor of Saposhnikovia divaricata seeds are all lower. The phenomenon is mainly caused by the variations among individuals and species characteristics. Low germination rate is firstly expressed as low germination rate of individuals, germination rate of 15 strains S. divaricata in trial varies from 28.0% to 92.0%, and overall germination rate of seeds is so low. Because the relieve dormancy time and aging time of every seed are not synchronous, results in all of seeds do not simultaneously entered fastigium of seeds germination. The initial time and duration of seed germination are all beyond two weeks. The mixture of 5~50 mg·kg -1 GA, 1~10 mg·kg -1 6-BA, 1% KNO 3 and 3% H 2O 2 can remarkably enhance germination rate of dormancy seeds, among these influential factors, GA and 6BA can release dormancy of S. divaricata seeds, however, 1%KNO 3 and 3%H 2O do not express this effect. Trace elements such as 10~100 mg·kg -1 Mn 2+, 10 mg·kg -1 Cu 2+ and 1 mg·kg -1 Mo, etc, can notably enhance germination rate of S. divaricata seeds. Hence, these trace elements were spurted into plants at green fruit period of S. divaricata, the germination rate of seeds is also increased. The green fruits were treated with GA and NAA, which also increase the germination rate of S. divaricata seeds. Reference | Related Articles | Metrics

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Morphology of Huperzia serrata and Its Stomata Characteristics under Different Light Treatments LI You-You;TANG Yuan-Jiang;LI Jing;ZHOU Yi;HUANG Heng-Yu* Bulletin of Botanical Research    2009, 29 (4): 411-416.   DOI: 10.7525/j.issn.1673-5102.2009.04.005 Abstract （1885）      PDF（pc） （389KB）（514）       Knowledge map    Save The morphological characteristics of Huperzia serrata(Thunb.) Trev. were described, and a few different characteristics were found. In addition, the relationship between the stomata characteristics and the illumination intensity was analyzed. The results showed the length, width and area of single stomata, the stomata density and the total stomata area increased when the illumination intensity kept rising within a certain limit. However, all of these stomata parameters decreased when the illumination intensity surpassed a certain value except the stomata density and the stomata index. The stomata of small sizes and high densities were the characteristics fitted for the arid conditions, indicting that this plant was ecological adaptive, but when it was under cultivation high illumination intensity should be avoided. Reference | Related Articles | Metrics

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A new species of genus Cycas from China FU Guo-Ai Bulletin of Botanical Research    2004, 24 (4): 387-388.   Abstract （554）      PDF（pc） （117KB）（379）       Knowledge map    Save A new species of the genus Cycas Linn.(Cycadaceae) Cycas lingshuigensis G.A.Fu from Hainan Island is described. Related Articles | Metrics

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Progress on the Metabolic Basis and Regulation Mechanism of Fruit Flavor Hua-Feng CHEN, Yu-Qing TANG, Ya-Jie PAN, Xiao-Rui GUO Bulletin of Botanical Research    2021, 41 (3): 474-480.   DOI: 10.7525/j.issn.1673-5102.2021.03.019 Abstract （1082）   HTML （75）    PDF（pc） （854KB）（988）       Knowledge map    Save Fruit flavor substances have the characteristics of organ specificity， development specificity， and environmental specificity， and their metabolic mechanism is the material basis of fruit regulation. Ethylene is an important signal molecule in the process of plant growth and development， and has a unique regulatory effect on the synthesis and metabolism of fruit flavor substances. With the maturity and development of multi-organization technology， the use of ethylene to regulate key enzyme activities and gene families and characteristic effect compound genes in the process of flavor substance metabolism to achieve fruit quality improvement has become one of the main methods of fruit flavor regulation. Based on the research of fruit flavors at home and abroad in recent years， this article summarizes the metabolic basis of fruit flavors， regulatory mechanisms， the response of flavors to ethylene， and the research progress of bioinformatics in fruit flavors， and proposes future regulatory directions Three prospects are presented， hoping to provide a certain theoretical reference for the improvement of fruit flavor and nutrition and the purpose of fruit regulation. Table and Figures | Reference | Related Articles | Metrics

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Research Progress on the Mechanism of Plant Hormone Regulating Yield and Latex Flow in Hevea brasiliensis Bingbing GUO, Mingyang LIU, Longjun DAI, Hong YANG, Lifeng WANG Bulletin of Botanical Research    2024, 44 (2): 161-167.   DOI: 10.7525/j.issn.1673-5102.2024.02.001 Abstract （67）   HTML （12）    PDF（pc） （537KB）（79）       Knowledge map    Save Rubber tree（Hevea brasiliensis） is one of the important tropical cash crops， and natural rubber（NR）， a secondary metabolite， is an important industrial raw material and strategic material in China. Phytohormones play important roles in regulating plant growth， development， germination， and environmental responses. Here， the research progress on application of four phytohormones， Ethylene， Abscisic acid， Brassinolides， and Gibberellin in the growth and development of rubber trees， NR biosynthesis， rubber yield， latex flow， and quality formation， was summarized in detail， and the application prospect of four phytohormones in mechanism research of improving rubber production and latex flow was looked forward to provide theoretical support for the rubber industry development. Reference | Related Articles | Metrics

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Preparation Process and Bioavailability Evaluation of β-cyclodextrin Inclusion Korean Pine Seed Oil ZU Shu-Chong Bulletin of Botanical Research    2020, 40 (2): 314-320.   DOI: 10.7525/j.issn.1673-5102.2020.02.020 Abstract （1085）      PDF（pc） （1255KB）（342）       Knowledge map    Save The single-factor method was used to optimize the preparation process of the Korean pine seed oil inclusion compound. Under the optimal conditions, the solidification rate of the Korean pine seed oil inclusion compound was 70.95%, and the oil content of the inclusion compound was 26.88%. The fatty acid composition, particle size, potential, and bioavailability of Korean pine seed oil and Korean pine seed oil inclusion complex were compared. There was no significant difference between the contents of each component of the Korean pine seed oil in the inclusion compound and that of the Korean pine seed oil, among which the content of pinolenic acid was in 14% to 16%. The average particle size of the Korean pine seed oil inclusion complex was 177.3±2.6 nm, and the potential was -33.01±1.4 mV. The blood drug concentration(CMAX), t1/2A, AUC and MRT were 1.27, 1.42, 1.56 and 1.04 times of the Korean pine oil inclusion complex group, respectively. The inclusion complex of Korean pine seed oil and aqueous solution formed an emulsion, the particle size decreased, the water solubility increased, the inclusion complex in vivo t1/2A and the average retention time(MRT) increased, and the absorption of pinolenic acid in vivo increased. Reference | Related Articles | Metrics

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Recent Advances on Salt Stress Sensitivity and Related Calcium Signals in Plants Jiaorao CHEN, Xu XU, Zhangli HU, Shuang YANG Bulletin of Botanical Research    2022, 42 (4): 713-720.   DOI: 10.7525/j.issn.1673-5102.2022.04.021 Abstract （697）   HTML （45）    PDF（pc） （1470KB）（474）       Knowledge map    Save Salt stress had a critical impact on plant growth and development， including osmotic stress and ion toxicity， which seriously damaged agricultural production and food security. Under salt stress， plant related receptors were stimulated to send Ca2+ enter the cytoplasmic matrix through the Ca2+ channels opened on the cell membrane and intracellular calcium storage membrane， resulting in the increase of intracellular Ca2+ concentration and calcium signal. Ca2+， as an important second messenger， transmited signals within and between plant cells， downstream， and caused a series of physiological responses to salt stress at different growth and development stages. Calcium signals mainly included calmodulin（CaM）， calmodulin like protein（CML）， calcium dependent protein kinase（CDPK） and calcineurin B-like protein（CBL） and CBL interacting protein kinase（CIPK）， and sensed and transmited specific calcium signal information to the downstream， and activated plant physiological response to salt stress. This paper reviewed how plants perceived salt stress stimulation and the mechanism of calcium signal regulation， and looked forward to the problems should be solved in this research field. Table and Figures | Reference | Related Articles | Metrics

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Screening and Phenotypic Identification of Seed Coat Pigmentation Mutants in Arabidopsis thaliana Na LI, Xiaonan WANG Bulletin of Botanical Research    2023, 43 (1): 59-68.   DOI: 10.7525/j.issn.1673-5102.2023.01.007 Abstract （3641）   HTML （34）    PDF（pc） （5322KB）（891）       Knowledge map    Save Flavonols play important roles in plant responses to various environmental stresses and in the regulation of seed coat development. In this study， a transparent seed coat mutant was obtained through ethyl methane sulfonate（EMS） mutagenesis screening， compared with wild-type Arabidopsis thaliana（Col-0）， the mature seed color of the mutant was yellow， and its phenotypic trait was controlled by a recessive single gene. Using map-based cloning and fine-mapping technology， the mutant gene TT4（At5G13930） was mapped to BAC of MAH20 on chromosome 5， and the 1 299th base C of the TT4 was mutated to T， resulting in the 324th amino acid glycine mutated to glutamic acid. TT4（transparent testa 4） encoded a structural gene for flavonoid synthesis， chalcone synthase（CHS）， and after mutation， the seed coat was transparent and the seed color was yellow， then the mutant was named tt4-1. The phenotype of brown seed coat was restored by functional complement mutants， which further demonstrated the important role of TT4 in regulating the development of seed coat color. The tissue expression analysis of TT4 was conducted by promoter coupling GUS assay， and it was found that TT4 gene was expressed in the roots， stems， leaves of plant seedlings and flowers respectively. Physiological phenotypic analysis showed that the mutant tt4-1 seeds germinated earlier， and the seedlings had shorter taproots， as well as more lateral roots and root hairs， larger stomatal opening and higher water loss rate in mature leaves compared with the wild type. This study would lay a theoretical basis for further elaborating the function of TT4 gene. Table and Figures | Reference | Related Articles | Metrics

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STUDY ON THE CHINESE WILD WOODY PEONIES (Ⅰ) NEW TAXA OF PAEONIA L.SECT. MOUTAN DC. Hong Tan, Zhang Jia-xun, Li Jia-jue, Zhao Wen-zhong, Li Ming-rui Bulletin of Botanical Research    1992, 12 (3): 223-234.   Abstract （734）      PDF（pc） （803KB）（616）       Knowledge map    Save Woody Peony is a kind of precious flower and medicinal tree endemicto China, which has had a cultivation history of over 1500 years. So far, there are over 500 cultivars in China. Most of the research on the classification of woody peony and thedescription and identification of peony species were conducted by scientistsof the western countries on the basis of the peony plants and specimensintroduced into England, USA and France etc. from China during the18th-19th century. In recent years, the author has conducted a series of investigation andstudy on Chinese wild woody peonies in Anhui, Henan, Shanxi, Shaanxi, Gansu, Sichuan and Yunnan Provinces of China. The present paper proposes 3 new species and 1 new status of Chinesewild woody peonies. This achievement leads to provide the scientific andtheoretical basis for the study on the origin and natural classification ofpeony cultivars and exploitation, protection, utilization of Chinese precious, rare and endangered gene resources of wild woody peonies as well as thehybridization of new cultivars and expansion of peony cultivated areaetc. Osti's Peony ( Paeonia ostii T. Hong et J. X. Zhang sp. nov.) The species name "ostii" is dedicated to Dr. Gian Lupo Osti, theItalian Vice President of International Dendrology Society. We do appr-eciate his friendly, valuable and precious help to promote the scientificresearch of Chinese wild woody peonies! Osti's Peony differs from Rock's Peony and its congeners are in moreor less lanceolate leaflets with entire margin, glabrous beneath and 4-7pairs of veins, the terminal leaflets 1-3 lobed;petals white or slightlytinged with dilutely reddish color, but without purple basal blotch;fila-ments, disks and styles dark purplish red all together. Jishan Peony ( Paeonia jishanensis T. Hong et W. Z. Zhao sp. nov.) It differs from P. suffruticosa Andr. subsp. spontanea (Rehd.) Haw & Lauener in its white flowers and without petaloid stamens. A. Rehder, an American dendrologist, identified and nominated it asa new variety ( P. suffruticosa Andr. var. spontanea Rehd.) in 1920, basedon the specimen No, 338 collected by W. Purdom in 1910 at a place loca-ted at 25 kilometers away from the west of Yanan. The original Latindescription of its main characteristics is:"floribus roseis, interdumstaminibus petaloideis praeditis" (flowers roseate, sometimes presence ofpetaloid stamens). In 1990, S. G. Haw L. A. Lauener changed Rehder's variety intosubspecies ( P. suffruticosa subsp. spontanea) and identified the white-flow-ered wild peony which is distributed over Majiagou Jishan County inShanxi Province at the alt. 1450m to the same subspecies. The author thinks that the petaloid stamens is one of the most important characteristics of Peony Cultivars originating from wild speciesafter cultivation. It shouldn't be confused with wild peony. Therefore, Haw's subspecies should be lowered and changed to cultivar. The JishanPeony is an undoubted wild woody peony species. Yanan Peony ( Paeonia yananensis T. Hong et M. R. Li sp. nov.) It's a rare and endangered wild woody peony species which has asuperficial resemblance to Rock's Peony being dark purplish blotched atthe base of petals, but is easily distinguished by few leaflets (up to 11), smaller and dilutely purplish roseate or white petals, reddish purple stigmaand disk etc. Rock's Peony [ Paeonia rockii (Haw & Lauener) T. Hong et J. J. Listat. nov.] It's identified and nominated by S. G. Haw & L. A. Lauener as a new subspecies ( P. suffruticosa Andr. subsp. rockii) based on "Rock'sVariety" which was bred from seeds collected by an American Geographer, Joseph Rock in 1925-1926 from a peohy with big white flower and darkpurple basal blotch in a lamasery of Choni County in the south of GansuProvince. The author of Genus Paeonia of Fl. Reip. Pop. Sin. was mistaken toidentify the peony with white flower and dark purple basal blotch whichis distributed over the south of Gansu and Mt. Taibai of Shaanxi Provincesetc. as Paeonia suffruticosa Andr. var. papaveracea (Andr.) Kerner. H. C. Andrews, a British plant taxonomist, studied a peony plant with white flower and dark purple basal blotch, which was introduced to England from Guangzhou, China and cultiuated in the garden of Sir Abraham Hume, and identified and nominated it as a new species， Paeonia papaveracea Andr. in 1806. After that, Kerner changed it as a variety, P. suffruticosa An dr. var. papaveracea (Andr.) Kerner in 1816.Bean，a British scientist, studied the peony planted in Sir Abraham Hume Garden. He thought it is neither a species nor a variety and reduced it as a cultirar, P. suffmticosa Andr. cv. ‘ Papaveracea’The author thinks that Paeonia suffruticosa Andr. is a name for a big group of various cultivars while Rock's Peony is a wild woody peony species from which originating a series of cultivars. So the author raised Haw's subspecies to species grade, Paeonia rockii (Haw & Lauener) T. Hong et J. J. Li stat. nov. Related Articles | Metrics

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Structural and Functional Analysis of R2R3 MYB Transcription Factor HbMYB88 FAN Song-Le, WANG Ji-Kun, XIE Gui-Shui, WANG Meng, WANG Li-Feng Bulletin of Botanical Research    2020, 40 (1): 106-116.   DOI: 10.7525/j.issn.1673-5102.2020.01.015 Abstract （2377）      PDF（pc） （1870KB）（614）       Knowledge map    Save The R2R3-MYB transcription factors are involved in biological processes, such as plant growth and development, hormone signaling transduction, and stress response, etc. To explore the structure and functions of the MYB family members in the rubber tree, we cloned the full-length cDNA of HbMYB88 from the leave of rubber tree Reyan 73397(RY73397). It is 1 848 bp in length, containing a 1 440 bp ORF which encodes 479 amino acid residues. The deduced amino acid sequence of HbMYB88 contains two SANT conserved domains, has HTH tertiary structure, share highly similarity to Arabidopsis AtMYB88, AtMYB124. AtMYB88 and AtMYB124, not divided into subfamilies, are related to drought stress response, etc. By qRT-PCR analysis, HbMYB88 was mainly expressed in the stem and flower of rubber tree, whilst its expressions in root, leaf, bark and latex were extremely low. The expression level of HbMYB88 in tissue culture seedling leave was significantly upregulated under the treatment of hydrogen peroxide(H 2O 2), and abscisic acid(ABA). These indicated that HbMYB88 has a relationship with stress response such as drought in rubber trees, which providing a foundation for further study of its structure and function. Reference | Related Articles | Metrics

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Comparative Transcriptome Analysis of Five Species of Lilium Xue-Yan ZHAO, Si-Feng LI, Guo-Qing BAI, Wei-Min LI Bulletin of Botanical Research    2021, 41 (4): 614-625.   DOI: 10.7525/j.issn.1673-5102.2021.04.017 Abstract （1045）   HTML （822）    PDF（pc） （3841KB）（539）       Knowledge map    Save Lilium are perennial herbaceous plants with important ornamental value and some species are edible and medicinal. In the present study， the transcriptome of four species of Lilium（L. pumilum， L. davidii， L. lancifolium and L. brownii var. viridulum） was sequenced respectively， and the genes which involved in flower development were analyzed. Combined with the previous transcriptome data of L. brownii， the orthologous genes were selected by comparative transcriptome analysis. Positive selections were screened and their environmental adaptability were analyzed respectively. 44 565， 51 413， 41 638 and 44 716 unigenes were obtained from L. pumilum， L. davidii， L. lancifolium and L. brownii var. viridulum respectively by assembly. In order to understand the function of unigene， the unigenes were compared and annotated in the public database. 13 genes related to the development of flower were identified. A total of 8 247 orthologous genes were obtained in five species， and Ka， Ks and Ka/Ks values were calculated. 33 pairs of homologous genes were strongly positive selected in five species. Subsequently， GO and KEGG enrichment analysis and gene function annotation were carried out， and it was found that these genes were mainly related to plant defense and biosynthesis. Table and Figures | Reference | Related Articles | Metrics

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Bioinformatic Analysis and Expression Pattern of LTP Family Genes in Populus davidiana × P. alba var. pyramidalis Qian Sun, Yuhang Wu, Yaxuan Zhang, Jingdan Cao, Jingjing Shi, Chao Wang Bulletin of Botanical Research    2022, 42 (2): 211-223.   DOI: 10.7525/j.issn.1673-5102.2022.02.006 Abstract （657）   HTML （46）    PDF（pc） （5129KB）（480）       Knowledge map    Save To lay a foundation for studying the molecular regulation mechanism of LTP family gene and molecular genetic improvement of Populus davidiana × P. alba var. pyramidalis， the expression pattern of LTP family genes from P. davidiana × P. alba var. pyramidalis was analyzed.The protein properties， multiple sequence alignments and phylogenetic tree analysis of LTP family members were performed to show their structural features. The P. davidiana × P. alba var. pyramidalis seedlings treated with gravity， salt and PEG stress respectively were subjected to quantitive real time PCR analysis. The expression patterns of PdbLTP family genes were carried out respectively. Eight PdbLTP genes and two subfamily PdbGLTP genes were obtained， and their CDS lengths were ranged from 294-396 bp. LTP family were hydrophobic proteins and had eight cysteine conserved structures， without transmembrane region. qRT-PCR analysis showed that the expression patterns of different PdbLTP family genes were different under gravity processing. Expression levels of PdbLTP1， PdbLTP3， PdbLTP5 and PdbLTP7 genes were up-regulated in tension wood compared to those in the normal wood. The transcripts levels of PdbLTP1， PdbLTP2， PdbLTP3， PdbLTP5 genes were abundant in stem. Expression of PdbLTP family genes was induced by salt stress except for PdbLTP5. PdbLTP1， PdbLTP2， PdbLTP3 and PdbLTP5 were up-regulated under drought stress. Different members of PdbLTP gene family play important roles in regulation of xylem development and tolerance to abiotic stress in Populus davidiana × P. alba var. pyramidalis. Table and Figures | Reference | Related Articles | Metrics