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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (5): 779-786.doi: 10.7525/j.issn.1673-5102.2023.05.014

• Molecular biology • Previous Articles     Next Articles

Effect of Expression of Aeβ-AS gene from Aralia elata on the Content of Saponins in Nicotiana tabacum L.

Qingqing HUO, Yuxin XIA, Jiale LI, Wei HAN, Shuya ZHANG, Zhe ZHANG, Meiling XIA, Wenhua GUO, Xiangling YOU()   

  1. Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040
  • Received:2023-03-19 Online:2023-09-20 Published:2023-09-05
  • Contact: Xiangling YOU E-mail:youxiangling@nefu.edu.cn
  • About author:HUO Qingqing(2002—),female,undergraduate student,research direction is medicinal plant cell engineering.
  • Supported by:
    Undergraduate Innovation Project of Northeast Forestry University(202210225097)


To investigate the effect of β-amyrin synthase gene(Aeβ-AS) on triterpenoid saponin synthesis of Aralia elata, the Aeβ-AS gene was cloned and genetically transformed into tobacco. The expression difference of Aeβ-AS gene in different parts of transgenic tobacco was analyzed, and the expression levels of Aeβ-AS gene, upstream and downstream key enzyme genes and total triterpene content were detected respectively. The results showed that the plant overexpression vector PROKⅡ-Aeβ-AS was successfully constructed and transferred into wild type tobacco, and seven transgenic lines were obtained and expressed at the mRNA level, and the expression level in leaves was higher than that in roots and stems. In transgenic tobacco, the expression levels of Aeβ-AS gene and its upstream and downstream key enzyme genes were higher than those of wild type, and the relative expression levels of NtFPS and NtSS genes of strain L21 were the highest, and the relative expression levels of NtSE and Aeβ-AS genes of strain L30 were the highest. Compared with wild-type tobacco, the total triterpene content of GM tobacco was significantly increased(1.1-1.6 times). The results showed that synthesis of Aeβ-AS gene and heterologous transformation in tobacco could significantly increase the total triterpene content in transgenic tobacco.

Key words: Aralia elata, Aeβ-AS gene, Nicotiana tabacum, total triterpenoids content

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