Effects of Three Kinds of Agrobacterium and Different Transformation Conditions on the Transient Expression of GFP in Nicotiana benthamiana

doi:10.7525/j.issn.1673-5102.2022.01.013

Abstract

Abstract:

By using Nicotiana benthamiana as plant material， the influence of the different Agrobacterium strains（LBA4404， EHA105， GV3101）， bacterial concentration and infection time on GFP（as the reporter gene） the fluorescence transient expression after transformation were analyzed respectively. The results showed that the expression of GFP with different Agrobacterium strains were various in the optimal concentration and time for transient： the highest transient expression efficiency with LBA4404 strain was achieved when the OD₆₀₀ of bacterial suspension was 0.8； while the highest transient expression efficiency with EHA105 and GV3101 strains was achieved when the OD₆₀₀ of the bacterial suspension reached 0.6. The level of transient expression by strain LBA4404 was the highest at 2 days later Agrobacterium infiltration while the level of transient expression by strains EHA105 and GV3101 was the highest at 4 days later Agrobacterium infiltration. The comparative analysis among these different strains showed that the transient expression efficiency by using LBA4404 strain was the highest. The above results indicated that Agrobacterium strains and transformation conditions such as bacterial concentration and infection time were important factors that affected the transient expression efficiency.

Key words: Nicotiana benthamiana, syringe infiltration, transient expression, GFP

CLC Number:

S572

Yuejing Zhang, Ying Li, Juanjuan Wang, Hailong Pang, Lingyun Jia, Hanqing Feng. Effects of Three Kinds of Agrobacterium and Different Transformation Conditions on the Transient Expression of GFP in Nicotiana benthamiana[J]. Bulletin of Botanical Research, 2022, 42(1): 121-129.

Figures/Tables 7

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