Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (1): 121-129.doi: 10.7525/j.issn.1673-5102.2022.01.013
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Yuejing Zhang, Ying Li, Juanjuan Wang, Hailong Pang, Lingyun Jia, Hanqing Feng()
Received:
2021-03-11
Online:
2022-01-20
Published:
2021-12-30
Contact:
Hanqing Feng
E-mail:fenghanq@nwnu.edu.cn
About author:
Zhang Yuejing(1995—),female,senior engineer,mainly engaged in plantphysiology research work.
Supported by:
CLC Number:
Yuejing Zhang, Ying Li, Juanjuan Wang, Hailong Pang, Lingyun Jia, Hanqing Feng. Effects of Three Kinds of Agrobacterium and Different Transformation Conditions on the Transient Expression of GFP in Nicotiana benthamiana[J]. Bulletin of Botanical Research, 2022, 42(1): 121-129.
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URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.01.013
Fig.2
expression of GFP in EHA105 bacteria at different concentrations and different infection timesA.Control group of 3 different Agrobacteria;B.Representative images of GFP expression in EHA105 bacteria at different concentrations(OD600 values were 0.2,0.4,0.6,0.8,1.0) and at different infection times(day 2,day 4 and day 6)
Fig.7
expression of GFP in 3 different agrobacterium strainsThe concentration and time combinations with the highest fluorescence expression of 3 different Agrobacterium strains were selected for comparison,EHA105 strain(OD600=0.6,day 4); LBA4404 strain(OD600=0.8,day 2);GV3101 strain(OD600=0.6,day 4)
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