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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 614-625.doi: 10.7525/j.issn.1673-5102.2021.04.017

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Comparative Transcriptome Analysis of Five Species of Lilium

Xue-Yan ZHAO, Si-Feng LI, Guo-Qing BAI, Wei-Min LI()   

  1. Shaanxi Engineering Research Centre for Conservation and Utilization of Botanical Resources,Xi’an Botanical Garden of Shaanxi Province,Institute of Botany of Shaanxi Province,Xi’an 710061
  • Received:2020-08-29 Online:2021-07-20 Published:2021-03-24
  • Contact: Wei-Min LI E-mail:763730163@qq.com
  • About author:ZHAO Xue-Yan(1986—),female,lecturer,doctor,Research interests: anatomical structure of plants and resource exploitation and utilization.
  • Supported by:
    Key technology R&D program of Shaanxi province(2018NY-88);Science and technology program of Shaanxi academy of sciences(2020K-28)


Lilium are perennial herbaceous plants with important ornamental value and some species are edible and medicinal. In the present study, the transcriptome of four species of LiliumL. pumilumL. davidiiL. lancifolium and L. brownii var. viridulum) was sequenced respectively, and the genes which involved in flower development were analyzed. Combined with the previous transcriptome data of L. brownii, the orthologous genes were selected by comparative transcriptome analysis. Positive selections were screened and their environmental adaptability were analyzed respectively. 44 565, 51 413, 41 638 and 44 716 unigenes were obtained from L. pumilumL. davidiiL. lancifolium and L. brownii var. viridulum respectively by assembly. In order to understand the function of unigene, the unigenes were compared and annotated in the public database. 13 genes related to the development of flower were identified. A total of 8 247 orthologous genes were obtained in five species, and Ka, Ks and Ka/Ks values were calculated. 33 pairs of homologous genes were strongly positive selected in five species. Subsequently, GO and KEGG enrichment analysis and gene function annotation were carried out, and it was found that these genes were mainly related to plant defense and biosynthesis.

Key words: Lilium, transcriptome, flower development gene, orthologous gene, positive gene

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