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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (4): 601-611.doi: 10.7525/j.issn.1673-5102.2023.04.013

• Molecular biology • Previous Articles     Next Articles

Cloning and Functional Analysis of EgrWAT1 Gene in Eucalyptus grandis

Haonan ZHANG1,2, Shanshan CHEN2,3, Jianmin XU2, Ping LUO2, Xiaoping WANG2, Zhiru XU1, Chunjie FAN2,3()   

  1. 1.School of Life Science,Northeast Forestry University,Harbin 150040
    2.Laboratory of State Forestry and Grassland Administration,Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou 510520
    3.State Key Laboratory of Forest Tree Genetics and Breeding,Northeast Forestry University/Chinese Academy of Forestry,Harbin/Beijing 150040/ 100091
  • Received:2022-07-13 Online:2023-07-20 Published:2023-07-03
  • Contact: Chunjie FAN E-mail:fanchunjie@caf.ac.cn
  • About author:ZHANG Haonan(1998—),female,postgraduate,mainly engaged in tree genetics and breeding reseach.
  • Supported by:
    Basic Scientific Research Funds of Chinese Academy of Forestry(CAFYBB2020ZB004)


In order to explore the role of WALLS ARE THIN(WAT1) in wood formation and response to stress in woody plants, bioinformatics toolswas used for analysis, and quantitative Real-time PCR(qRT-PCR) was used to investigate the expression patterns of EgrWAT1L and EgrWAT1S in different tissues, internodes and in response to different stresses, and the gene EgrWAT1S and its other transcript EgrWAT1L were cloned from Eucalyptus grandis. The results showed that the EgrWAT1S was highly expressed in phloem, while EgrWAT1L was mainly expressed in roots, and the expression patterns were significantly different under methyl jasmonate(MeJA) and salicylic acid(SA) treatment, salt stress, phosphorus(P) and boron(B) deficiency, and even opposite under MeJA and SA. These results suggested that EgrWAT1L might affect EgrWAT1S expression through transcriptional regulation and further protein translation in response to hormone and stress treatments. The studies provided a basis for further elucidate the function in the growth and development of EgrWAT1 gene and also provided a possibility for future molecular breeding of Eucalyptus.

Key words: Eucalyptus grandis, WAT1, splice variant, gene cloning, expression pattern

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