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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (4): 562-571.doi: 10.7525/j.issn.1673-5102.2023.04.009

• Physiology and Ecology • Previous Articles     Next Articles

Exogenous Sucrose Affected AtKEA1 and AtKEA2 to Regulate Root Growth of Seedling in Arabidopsis thaliana

Sheng ZHENG1,2(), Haixia GAO1, Min SU1, Shanghuan LU1, Tengguo ZHANG1, Guofan WU1   

  1. 1.College of Life Sciences,Northwest Normal University,Lanzhou 730070
    2.Academy of Plateau Science and Sustainability,Qinghai Normal University,Xining 810016
  • Received:2022-12-13 Online:2023-07-20 Published:2023-07-03
  • Contact: Sheng ZHENG E-mail:zhengsheng@nwnu.edu.cn
  • About author:ZHENG Sheng(1984—),male,Ph.D,associate professor,mainly engaged in functional research of plant ion transporters.E-mail:zhengsheng@nwnu.edu.cn
  • Supported by:
    National Natural Science Foundation of China(31860054);Northwest Normal University young teachers research capacity promotion plan(NWNULKQN2020-26)


To explore the function roles of K+-efflux-antiporters KEA1 and KEA2 in Arabidopsis thaliana, wild type and kea1kea2 knock-down mutants of Arabidopsis thaliana were used as materials to investigate the effects of KEA1 and KEA2 on plant growth and development. Phenotypic analysis, propidium iodide staining to observe the structure of roots, high performance liquid chromatography to determine endogenous sugar content, transcriptome sequencing and qRT-PCR to analyze the relative expression levels of related genes, histochemical staining to detect the distribution of superoxide anion in leaves were performed respectively. The results showed that the root length of the kea1kea2 mutant was significantly shorter than that of the wild-type Col-0 plant in the absence of sucrose. Further observation showed that compared with the wild-type, the root meristem zone of kea1kea2 mutant was shorter, the endogenous sucrose content was decreased, and more O2·- was distributed in the leaves of the kea1kea2 mutant. However,when 30 g·L-1 sucrose was exogenously added, there was no significant difference in root length between the wild-type and kea1kea2 mutant. Transcriptome analysis revealed that the expression of many key genes involved in sucrose signals and root growth were repressed in the kea1kea2 mutant. In summary, the results suggested that sucrose might affect AtKEA1and AtKEA2 to regulate root growth in Arabidopsis seedlings.

Key words: Arabidopsis thaliana, K+-efflux antiporter, KEA, sucrose, root growth

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