Analysis of Non-Cellulosic Monosaccharide Composition of Poplar Cell Wall by HPAEC-PAD

doi:10.7525/j.issn.1673-5102.2023.05.015

Abstract

Abstract:

To establish an ion exchange chromatography-pulsed amperometric detection （HPAEC-PAD） method for non-cellulosic monosaccharides in poplar（Populus spp.） cell walls， the 9 main cell wall monosaccharides could be base-line separated in one run by optimizing the separation conditions. Dionex CarbiPac^TM PA100 （4 mm×250 mm） with sodium hydroxide analytical column was used for gradient elution at flow rate is 1.0 mL·min^-1 and the column temperature of 30 ℃ was used for the determination of monosaccharide fraction in the cell wall of different poplar species. The results showed that the 9 monosaccharides had a good linear relationship in the linear range of 0.5-150.0 mg·L^-1， the correlation coefficient R² was 0.999 0-0.999 3， the reproducibility（RSD） of the method was 1.09%-3.96%， and the recovery rate by standard addition was 91.32%-109.25%. The detection limit was 1.57×10^-3-1.41×10^-2 mg·L^-1. Galacturonic acid was the highest composition followed by xylose， and the glucuronic acid content was the lowest monosaccharide. The method developed in this study was widely applicable and simple， and could be used for the detection of monosaccharides in the cell walls of poplar and other species.

Key words: poplar, cell wall, ion chromatography, neutral sugar, uronic acid

CLC Number:

S792.11

Xiaoling JIN, Huimin WU, Chaofeng YANG, Jin ZHANG, Mengzhu LU, Wei ZENG. Analysis of Non-Cellulosic Monosaccharide Composition of Poplar Cell Wall by HPAEC-PAD[J]. Bulletin of Botanical Research, 2023, 43(5): 787-793.

Figures/Tables 8

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Table 1

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标准品 Standards	线性范围 Linear range /（mg·L^-1）	线性方程 Linear equation	相关系数 R²	检出限 Limit of detection /（mg·L^-1）	定量限 Limit of quantification /（mg·L^-1）	重现性 RSD（n=6） /%	保留时间 Retention time /min
岩藻糖 L-fucose	0.5~5.0	y=0.901 9x+0.067 5	0.999 2	1.57×10^-3	4.71×10^-3	1.09	5.43
鼠李糖 L-rhamnose	0.5~5.0	y=0.507 3x+0.024 2	0.999 3	5.77×10^-3	1.73×10^-2	1.76	11.72
阿拉伯糖 L-arabinose	5.0~25.0	y=0.865 4x+1.697 3	0.999 1	3.21×10^-3	9.63×10^-3	2.36	12.37
半乳糖 D-galactose	5.0~30.0	y=1.011 7x+1.608 6	0.999 0	2.14×10^-3	6.42×10^-3	1.64	16.00
葡萄糖 D-glucose	2.0~15.0	y=1.143 1x+0.676 7	0.999 1	3.49×10^-3	1.05×10^-2	1.75	18.07
木糖 D-xylose	5.0~40.0	y=0.7050x+3.079 2	0.999 0	6.28×10^-3	1.88×10^-2	2.11	21.68
甘露糖 D-mannose	0.5~5.0	y=0.803 7x-0.026 2	0.999 2	1.41×10^-2	4.22×10^-2	3.07	22.76
半乳糖醛酸 D-galacturonic acid	40.0~150.0	y=0.724 8x-4.107 8	0.999 1	7.76×10^-3	2.32×10^-2	3.84	33.07
葡萄糖醛酸 D-glucuronic acid	0.5~5.0	y=0.903 4x-0.101 8	0.999 3	5.17×10^-3	1.55×10^-2	3.96	35.89

分析物 Analytes	样品质量浓度 Found/（mg·L^-1）	加标量 Add/（mg·L^-1）	测定值 Measured value/（mg·L^-1）	加标回收率 Recovery/%	重现性 RSD（n=3）/%
岩藻糖 L-fucose	2.40	2.5	5.03	105.52	1.90
鼠李糖 L-rhamnose	1.81	2.0	3.97	109.25	4.86
阿拉伯糖 L-arabinose	8.42	10.0	17.70	91.51	2.92
半乳糖 D-galactose	13.74	15.0	29.61	105.80	1.69
葡萄糖 D-glucose	4.73	5.0	10.00	105.83	2.36
木糖 D-xylose	13.60	10.0	22.42	91.32	3.83
甘露糖 D-mannose	0.78	0.8	1.56	98.01	2.00
半乳糖醛酸 D-galacturonic acid	72.05	70.0	140.60	97.98	1.69
葡萄糖醛酸 D-glucuronic acid	1.57	1.5	3.15	105.19	3.96

分析物 Analytes	84K杨 84K poplar /（mg·L^-1）	山新杨 Shanxin poplar /（mg·L^-1）	银中杨 Yinzhong poplar /（mg·L^-1）	717杨 717 poplar /（mg·L^-1）	南林895杨 Nanlin 895 poplar /（mg·L^-1）
岩藻糖 L-fucose	3.40	3.28	2.48	3.37	2.40
鼠李糖 L-rhamnose	1.15	1.87	2.06	1.39	1.81
阿拉伯糖 L-arabinose	12.72	13.60	12.04	6.65	8.41
半乳糖 D-galactose	15.93	19.59	15.62	17.56	13.74
葡萄糖 D-glucose	9.04	8.71	6.95	9.48	4.73
木糖 D-xylose	35.37	20.03	14.69	30.19	13.60
甘露糖 D-mannose	1.73	1.45	1.94	1.81	0.78
半乳糖醛酸 D-galacturonic acid	79.90	84.71	81.87	44.85	72.05
葡萄糖醛酸 D-glucuronic acid	2.74	2.25	2.07	0.86	1.57

杨树种类 Poplar varieties	岩藻糖 L-fucose /%	鼠李糖 L-rhamnose /%	阿拉伯糖 L-arabinose /%	半乳糖 D-galactose /%	葡萄糖 D-glucose /%	木糖 D-xylose /%	甘露糖 D-mannose /%	半乳糖醛酸 D-galacturonic acid /%	葡萄糖醛酸 D-glucuronic acid /%
84K杨 84K poplar	2.44	0.74	9.99	5.92	5.92	27.78	1.13	44.41	1.66
山新杨 Shanxin poplar	2.60	1.33	11.77	6.28	6.29	17.33	1.05	51.86	1.51
银中杨 Yinzhong poplar	1.95	1.62	10.36	11.20	4.98	12.64	1.39	54.48	1.38
717杨 717 poplar	2.42	1.26	7.33	8.70	8.71	33.25	1.66	34.96	0.73
南林895杨 Nanlin 895 poplar	2.22	1.67	8.51	11.58	3.99	13.76	0.66	56.37	1.23

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