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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (5): 787-793.doi: 10.7525/j.issn.1673-5102.2023.05.015

• Phytochemistry • Previous Articles     Next Articles

Analysis of Non-Cellulosic Monosaccharide Composition of Poplar Cell Wall by HPAEC-PAD

Xiaoling JIN, Huimin WU, Chaofeng YANG, Jin ZHANG, Mengzhu LU, Wei ZENG()   

  1. The State Key Laboratory of Subtropical Silviculture,College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300
  • Received:2022-12-13 Online:2023-09-20 Published:2023-09-05
  • Contact: Wei ZENG E-mail:zengw@zafu.edu.cn
  • About author:JIN Xiaoling(1998—),female,graduate student,mainly engaged biochemical analysis of plant metabolites.
  • Supported by:
    Zhejiang Provincial “14th Five-Year” Forestry Breeding Collaborative Grant(2021C02070-1);Natural Science Foundation of China(31971619);Discipline Innovation(111 Project,D18008)


To establish an ion exchange chromatography-pulsed amperometric detection (HPAEC-PAD) method for non-cellulosic monosaccharides in poplar(Populus spp.) cell walls, the 9 main cell wall monosaccharides could be base-line separated in one run by optimizing the separation conditions. Dionex CarbiPacTM PA100 (4 mm×250 mm) with sodium hydroxide analytical column was used for gradient elution at flow rate is 1.0 mL·min-1 and the column temperature of 30 ℃ was used for the determination of monosaccharide fraction in the cell wall of different poplar species. The results showed that the 9 monosaccharides had a good linear relationship in the linear range of 0.5-150.0 mg·L-1, the correlation coefficient R2 was 0.999 0-0.999 3, the reproducibility(RSD) of the method was 1.09%-3.96%, and the recovery rate by standard addition was 91.32%-109.25%. The detection limit was 1.57×10-3-1.41×10-2 mg·L-1. Galacturonic acid was the highest composition followed by xylose, and the glucuronic acid content was the lowest monosaccharide. The method developed in this study was widely applicable and simple, and could be used for the detection of monosaccharides in the cell walls of poplar and other species.

Key words: poplar, cell wall, ion chromatography, neutral sugar, uronic acid

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