Fig.1

Comparison of mature seed color between WTand mutant tt4-1

Table 1

The primary mapping results of the mutant gene

Table 2

The fine positional mapping statistical results of the mutant gene

Fig.2

Sequencing results of mutant gene and identification of mutated geneA.The sequencing and results of blast（With the red circle that mutations for bases）；B.The sequence of normal amino acid and the mutations of amino acids of AT5G13930（With the red circle for normal amino acids，black arrow for the change of the amino acids），and Glycine（Gly，G） mutates to glutamate（Glu，E）；C.The Seed Coat Pigmentation of WT，tt4-1 and pTT4：：TT4-GFP tt4-1，and the mutated gene was indeed TT4

Fig.3

Construction of tissue expression vector of TT4A.TT4 promoter sequence map；B.pCAMBIA1391 vector map；C-D.Construction of pCAMBIA1391 vector with TT4 promoter sequence and verification of double digestion

Fig.4

Expression pattern and Tissue and organ localizationof TT4 geneA.Tissue expression of TT4 gene in transgenic seedlings of Arabidopsis thaliana grown for 7 d；B-F.Tissue expression of TT4 gene in cotyledon and stem（B），root hair zone（C），root mature zone（D），root tip and meristem（E） and flower（F，G）

Table 3

Comparison of seed traits between WT and mutant tt4-1

Fig.5

Comparison of seed shape between WT and mutant tt4-1

Fig.6

The phenotype analysis between WT and mutant tt4-1 under normal growth conditionA. Three weeks of plant growth in the Soil；B. Five weeks of plant growth in the Soil；C. Mature plants；D. Immature fruit pod

Fig.7

The germination rate between WT and mutant tt4-1 under normal growth condition

Fig.8

Comparison of lateral roots between WT and mutant tt4-1 in MS mediumA.The root growth in 0.8% MS culture；B.The number analysis of lateral root cultured in medium when agar concentrations were 0.6%，0.8%，1.0% and 1.2% respectively

Fig.9

Comparison of leaf temperature and Water loss rate between WT and mutant tt4-1A.The difference of WT and tt4-1 on surface temperature of two weeks seedlings；B.Water loss rate analysis of WT and tt4-1 under normal growth condition