Transcriptome Analysis in Vascular Cambium and Xylem of Paulownia tomentosa

doi:10.7525/j.issn.1673-5102.2021.03.005

Abstract

Abstract:

We obtained about 16.35 Gb transcriptome data in total from vascular cambium and xylem zone of Paulownia tomentosa by Illumina Hiseq^TM 2500 sequencing. After assembled， 104 432 unigenes were obtained， the average length of unigene were 662 nt. Then， the unigenes were blastn and annotated by public databases， and 40 789（Nr： 39.05%）， 31 675（NT：30.33%）， 15 539（COG：14.87%）， 29 168（GO：27.93%）， 16 316（KEGG：15.62%）， 30 499（SwissProt：29.20%） and 28 828（Pfam：27.6%） unigenes were annotated respectively. By GO analysis， 29 168 unigenes were potentially involved in 55 functional categories of biological processes， cellular components， and molecular functions respectively. By COG analysis， 15 539 unigenes were classified into 25 functional groups； whereas， by KEGG analysis， 16 316 unigenes were assigned to 130 known pathways. In addition， 16 118 simple sequence repeat（SSR） loci were determined in the transcriptome of P.tomentosa. This study provided a basic data for further exploration of important functional genes in P. tomentosa.

Key words: Paulownia, vascular cambium, high-throughput sequencing, transcriptome

CLC Number:

S792.43

Yun TENG, Zong-Bo QIU, Wen-Li WANG, Xue-Ru ZHANG. Transcriptome Analysis in Vascular Cambium and Xylem of Paulownia tomentosa[J]. Bulletin of Botanical Research, 2021, 41(3): 354-361.

Figures/Tables 6

Table 1

Table 2

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Table 3

Fig.3

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长度区间 Length interval	转录本 Transcript	单基因簇 Unigene
200~500	77 452	68 865
500~1 000	26 725	17 742
1 000~2 000	24 155	10 848
>2 000	17 549	6 977
总数量Total number	145 881	104 432
总长度Total length	129 567 733	69 112 491
N₅₀长度N₅₀ length	1 605	1 105
平均长度Mean length	888	662

数据库 Database	注释Unigenes数 Number of annotated Unigenes	占注释Unigenes百分数 Percentage of annotated Unigenes (%)
注释到Nr Annotated in Nr	40 789	39.05
注释到NT Annotated in NT	31 675	30.33
注释到SwissProt Annotated in SwissProt	30 499	29.20
注释到GO Annotated in GO	29 168	27.93
注释到Pfam Annotated in Pfam	28 828	27.6
注释到COG Annotated in COG	15 539	14.87
注释到KEGG Annotated in KEGG	16 316	15.62
注释到至少一个数据库 Annotated in at least one database	48 214	46.16
注释到所有数据库 Annotated in all database	7 386	7.07
Unigenes总量 Total Unigenes	104 432	100

KEGG通路 KEGG pathway	注释基因 Annotated genes	占总基因数的比例 Percentage of total genes(%)	通路ID Pathway ID
核糖体Ribosome	1 216	7.45	ko03010
植物病原体相互作用Plant-bacterium warfare	646	3.96	ko04626
碳代谢Carbon metabolism	638	3.91	ko01200
氨基酸的生物合成Biosynthesis of amino acids	559	3.43	ko01230
内质网蛋白加工Protein processing in endoplasmic reticulum	533	3.27	ko04141
剪接Spliceosome	396	2.43	ko03040
淀粉和蔗糖代谢Starch and sucrose metabolism	384	2.35	ko00500
RNA运输 RNA transport	371	2.27	ko03013
氧化磷酸化Oxidative phosphorylation	370	2.27	ko00190
植物激素信号转导Plant hormone signal transduction	361	2.21	ko04075
胞吞作用Endocytosis	325	1.99	ko04144
糖酵解/糖异生Glycolysis/gluconeogenesis	298	1.83	ko00010
嘌呤代谢Purine metabolism	275	1.69	ko00230
氨基糖和核苷酸糖代谢Amino sugar and nucleotide sugar metabolism	268	1.64	ko00520
苯丙素生物合成Phenylpropanoid biosynthesis	266	1.63	ko00940
泛素介导的蛋白水解Ubiquitin mediated proteolysis	257	1.58	ko04120
吞噬Phagocytosis	248	1.52	ko04145
丙酮酸代谢Pyruvate metabolism	220	1.35	ko00620
RNA降解 RNA degradation	220	1.35	ko03018
mRNA监视通路 mRNA surveillance pathway	217	1.33	ko03015

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