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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (3): 419-428.doi: 10.7525/j.issn.1673-5102.2021.03.012

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Screening and Expression Analysis of Different Genes for Oleoresin Production in the Specific Period of Pinus elliottii×P.caribaea by RNA-Seq Technology

Chen-Chen ZHOU1,2, Yi-Liang LI1,2(), Zhe WANG1,2, Fen-Cheng ZHAO1,2, Sui-Ying ZHONG3, Chang-Ming LIN3, Zhi-Qiang TAN3, Fu-Ming LI3, Hui-Shan WU1,2, Wen-Bing GUO1,2, Fang-Yan LIAO1,2, Jian-Ge WANG4   

  1. 1.Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520
    2.Guangdong Academy of Forestry,Guangzhou 510520
    3.Taishan Hongling Seed Orchard,Taishan 529200
    4.Jiangxi Agricultural University/College of Landscape and Art,Nanchang 330045
  • Received:2020-04-08 Online:2021-05-20 Published:2021-03-24
  • Contact: Yi-Liang LI E-mail:yiliangli@sinogaf.cn
  • About author:ZHOU Chen-Chen(1993—),female,postgraduate student,major in tree genetic improvement.
  • Supported by:
    Provincial Science and Technology Plan Project of Guangdong Province(2017B020232001);Guangdong Forestry Science and Technology Innovation Special Fund Project(2018KJCX017)


The experiment was conducted to explore the differential expression of genes which regulate oleoresin synthesis and transport in Pinus elliottii×P. caribaea. At the peak of oleoresin production, P. elliottii×P. caribaea with similar growth but significant differences in oleoresin production of plants were selected, and secondary xylem tissues were collected for high-throughput sequencing platform. The results showed that 594 108 382 clean reads were generated by sequencing. 190 514 unigenes were obtained by de novo splicing, and 304 unigenes were related to the synthesis of terpenoids. The 414 unigenes were obtained, differential expression analysis, including 183 unigenes up-regulated and 231 unigenes down regulated. The genes encoding CYPs, ABC transport proteins, PLR, Strictosidine synthase activity increased in high oleoresin-yielding, while the genes encoding prenyltransferase activity increased in low oleoresin-yielding. This study enriched the transcript information of P. elliottii× P. caribaea, and provided reference value for further study of the molecular mechanism of the difference in P. elliottii×P. caribaea and Pines plant resin production.

Key words: Pinus elliottii×P. caribaea, transcriptome sequencing, oleoresin production genes, qRT-PCR

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