Dynamics of Endophytic Bacterial Communities in Highland Barley Seeds at Different Growth Stages

doi:10.7525/j.issn.1673-5102.2025.06.013

Abstract

Abstract:

Highland barley is a grain crop adapted to alpine environments and plays a crucial role in agricultural sustainability and ecosystem stability．To elucidate the dynamic changes in endophytic bacterial communities in highland barley seeds across growth stages，seed samples from four varieties were collected and analyzed using 16S rRNA high-throughput sequencing．Alpha diversity was assessed using Shannon，Simpson，Chao1 and ACE indices，and community composition and its succession across growth stages were analyzed．The results showed that the diversity of endophytic bacterial communities in highland barley seeds remained relatively stable among varieties but significantly decreased during the grain-filling stage．Proteobacteria was the dominant phylum at all growth stages，with other dominant phyla including Bacteroidota，Firmicutes，and Actinobacteriota．The relative abundance of these phyla varied significantly with growth stages and exhibited variety-specific characteristics．Acinetobacter was the dominant genus at the seed stage，Bacillus and Pseudomonas prevailed during the grain-filling stage，and Flavobacterium and Lactobacillus were most abundant at maturation．The results also indicated that genotype significantly influenced the structure of endophytic bacterial communities，resulting in compositional differences among varieties．The community composition and diversity characteristics at different developmental stages suggest that endophytic bacteria may play crucial promotive and protective roles in the growth and development of hulless barley by facilitating nutrient acquisition， synthesizing plant growth hormones， enhancing tolerance to environmental stresses， and suppressing pathogenic microorganisms， thereby providing a scientific basis for further elucidation of their ecological functions and molecular regulatory mechanisms.

Key words: highland barley, seeds, endophytic bacteria, diversity, high-throughput sequencing

CLC Number:

S181.4

Lan WANG, Di ZHOU, Zhao XUE, Dongxu WANG, Xiaofang GUO, Ji DE. Dynamics of Endophytic Bacterial Communities in Highland Barley Seeds at Different Growth Stages[J]. Bulletin of Botanical Research, 2025, 45(6): 965-974.

Figures/Tables 5

Table 1

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品种 Variety	生长时期 Growth stage	香农指数 Shannon index	辛普森指数 Simpson index	Chao1指数 Chao1 index	ACE指数 ACE index
藏青2000 Zangqing 2000	种子期Seed stage	5.40±0.30^a	1.00±0.01^a	170.00±68.90^a	187.00±77.40^a
	灌浆期Grain-filling stage	2.30±0.50^a	0.70±0.20^a	20.80±8.30^a	26.70±15.10^a
	成熟期Maturity stage	2.80±2.10^a	0.60±0.50^a	37.90±30.10^a	—
藏青320 Zangqing 320	种子期Seed stage	4.10±1.10^a	0.90±0.10^a	62.90±39.60^a	70.30±42.50^a
	灌浆期Grain-filling stage	2.50±1.20^a	0.70±0.30^a	15.10±5.20^a	16.90±6.52^a
	成熟期Maturity stage	4.90±0.30^a	0.90±0.01^a	102.00±10.50^a	124.00±19.90^a
喜马拉雅22号 Himalayan 22	种子期Seed stage	3.58±1.40^a	0.79±0.16^a	56.10±33.60^a	58.70±41.90^a
	灌浆期Grain-filling stage	0.82±0.56^a	0.28±0.25^a	17.10±5.38^a	22.70±7.84^a
	成熟期Maturity stage	4.62±0.56^a	0.94±0.03^a	68.70±24.50^a	96.80±31.60^a
藏青13-5171-7 Zangqing 13-5171-7	种子期Seed stage	3.59±0.79^a	0.85±0.05^a	46.70±16.00^a	48.90±19.50^a
	灌浆期Grain-filling stage	1.08±0.59^a	0.41±0.26^a	4.50±0.87^a	6.47±1.63^a
	成熟期Maturity stage	4.16±1.02^a	0.88±0.10^a	56.10±32.80^a	71.30±46.70^a