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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (1): 119-129.doi: 10.7525/j.issn.1673-5102.2021.01.015

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To Explore the Transcription Factor in Response to Low Temperature Stress in Nicotiana alata by Transcriptome Sequencing

Lin LU1, Shang-Yu YANG2, Wei-Dong LIU1, Li-Ming LU2()   

  1. 1.College of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130
    2.College of Agronomy,Sichuan Agricultural University,Chengdu 611130
  • Received:2020-03-07 Online:2021-01-20 Published:2021-01-05
  • Contact: Li-Ming LU E-mail:louis_luliming@126.com
  • About author:LU Lin(1965—),female,associate professor,major in landscape plants study.
  • Supported by:
    Science and Technology Project of Sichuan Provincial Department of Education(12ZA272)


In order to identify the transcription factors involved in the low temperature stress of Nicotiana alata seedlings, 4℃ low temperature was carried out, and samples of the seedlings were collected in 12-hour treatment, then the total RNA was extracted, and high-throughput sequencing technology was used to sequence the transcriptome. For differentially expressed genes, GO and KEGG analysis were applied, the transcription factors involved were classified, and the results of transcriptome sequencing were verified by qRT-PCR. There were 8 388 genes(P<0.01) whose expression changed more than twice after low temperature treatment, of which 4 229 genes were up-regulated and 4 159 genes were down regulated. The functions of these differentially expressed genes were classified into three categories: biological process, cell components and molecular functions, 69 items in go entries, and were significantly enriched in 40 KEGG metabolic pathways. At the same time, under low temperature stress, the expression of 118 transcription factors in N.alata changed significantly, among which 82 genes were up-regulated and 36 genes were down regulated. These transcription factors belonged to 28 families, of which 19 genes to NAC family, 16 genes to ERF family, 15 genes to MYB family and 15 genes to WRKY family. The results provided a reference for the study of the molecular mechanism of low temperature response in N.alata.

Key words: Nicotiana alata, transcriptome, low temperature stress, transcription factors

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