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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (6): 886-896.doi: 10.7525/j.issn.1673-5102.2020.06.011

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Identification of Key Enzyme Genes Involved in Anthocyanin Synthesis pathway in Clinopodium gracile by Transcriptome Analysis

Li-Qiang ZHAO1,2, Chun-Miao SHAN1,2, Sheng-Xiang ZHANG1,2, Yuan-Yuan SHI1,2, Ke-Long MA3, Jia-Wen WU2,4,5()   

  1. 1.Graduate School,Anhui University of Chinese Medicine,Hefei 230012
    2.Key Laboratory of Xin’an Medicine,Ministry of Education,Anhui University of Chinese Medicine,Hefei 230038
    3.Clinical College of Integrated Traditional Chinese and Western Medicine,Anhui University of Chinese Medicine,Hefei 230012
    4.Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement,Hefei 230012
    5.Anhui Academy of Chinese Medicine,Hefei 230012
  • Received:2019-11-19 Online:2020-11-20 Published:2020-11-04
  • Contact: Jia-Wen WU E-mail:wujiawen@ahtcm.edu.cn
  • About author:ZHAO Li-Qiang(1995—),male,master degree candidate,mainly engaged in transcriptomics research.
  • Supported by:
    The Natural Science Research Grant of Higher Education of Anhui Province(KJ2018ZD028);Sustainable Utilization Project of Chinese Medicine Resources(2060302);National Natural Science Foundation of China(81874431);National College Students’ Innovation and entrepreneurship Training Program,China(201810369050)


In order to further understand the pathway of anthocyanin synthesis, the four tissues of C.gracile, namely, root, stem, leaf and flower, were sequenced using BGISEQ-500 platform. 128 856 unigenes were assembled and 40 unigenes encoding six key enzymes involved in the anthocyanin biosynthesis were identified. Homologous alignment and structure simulation of bifunctional dihydroflavonol 4-reductase(DFRs) were carried out, which showed that they have well-conserved sequences, spatial structures and NAD+ binding sites. The DFR structure model showed a tetrameric structure with Rossmann-like α-helixes/β-sheets/α-helixes sandwich fold.

Key words: Clinopodium gracile, anthocyanin, transcriptome, unigene, DFR

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