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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (3): 362-371.doi: 10.7525/j.issn.1673-5102.2021.03.006

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Identification of Knockout of BRI1 Mutant in Arabidopsis Mediated by CRISPR/Cas9

Guo-Fan WU1, Hong-Bin CHENG1, Yu-Jun WU2, Juan SHEN1, Wang-Ze WU1()   

  1. 1.College of Life Sciences,Northwest Normal University,Lanzhou 730070
    2.College of Life Sciences,Lanzhou University,Lanzhou 730030
  • Received:2020-04-25 Online:2021-05-20 Published:2021-03-24
  • Contact: Wang-Ze WU E-mail:wangzew78@sina.cn
  • About author:WU Guo-Fan(1971—),male,associate professor,master instructor,is engaged in the function and application of SERKs gene family.
  • Supported by:
    the National Natural Science Foundation of China(31860113);MOE Key Laboratory of Cell Activities And Stress Adaptations(lzujbky-2017-kb05)


CRISPR/Cas9 gene editing technology was used to specificity edit the BRI1 of Arabidopsis thaliana to creation new BRI1 mutants. We analyzed the sequence of BRI1 inthe obtained transgenic plants, the results showed that as one new BRI1 mutant due to the insertion of new bases, the normal BRI1 protein is terminated prematurely. This mutant have the similar phenotype as bri1-710, and both mutants show impaired response to BL treatment compared with wild-type. Those results suggested that the N-terminal of BRI1 may play a role in the BR signaling pathway. Therefore, this study could provide a reliable reference for further studies on the BRI1 function of A. thaliana and other homologous species.

Key words: CRISPR/Cas9, gene knockout, Arabidopsis, BRI1.

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