Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (3): 424-436.doi: 10.7525/j.issn.1673-5102.2022.03.012

• Molecular biology • Previous Articles     Next Articles

Transcriptome Analysis of Hemerocallis fulva Leaves Respond to Low Temperature Stress

Dongmei HUANG, Ying CHEN, Lu BAI, Di’an NI, Yiyang XU, Zhiguo ZHANG, Qiaoping QIN()   

  1. School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418
  • Received:2020-12-18 Online:2022-05-20 Published:2022-05-20
  • Contact: Qiaoping QIN E-mail:qinqp@sit.edu.cn
  • About author:HUANG Dongmei(1997—),female,postgraduate,major in landscape plants and germplasm innovation.
  • Supported by:
    Science and Technology agriculture Project of Shanghai Agricultural Commission(2019-02-08-00-08-F01107)


Low temperature is a kind of abiotic stress in the growth process of Hemerocallis fulva. The transcriptome data of Hemerocallis fulva leaves under cold treatment groups (10, 5, 0 ℃) and control group (15℃) was compared, and a total of 2 457 DEGs were screened, including 1 253 up-regulated genes and 1 204 down-regulated genes. The results showed that differentially expressed genes(DEGs) were mainly concentrated in 49 GO processes such as cellular process, metabolic process and catalytic activity, and 42 KEGG metabolic pathways, including metabolic pathways, biosynthesis of secondary metabolites and plant hormone signal transduction. Among them, the DEGs involved in plant hormone signal transduction pathway changed in varying degrees, GH3.10 gene was increased to 13.624 times higher than the control group, IAA1 gene was down-regulated to 0.120 times; the DEGs involved in soluble sugar synthesis pathway also changed by 0.076-28.114 times. Then, 29 DEGs shared by treatment groups were analyzed by heat map and gene regulation networks. Based on the position of genes in gene regulation networks, the roles of ABCF5OFPs and SWEETs in response to cold were analyzed respectively. The results of this study provide a theoretical basis for further exploring the key genes of low temperature response, germplasm development and molecular breeding of Hemerocallis fulva.

Key words: Hemerocallis fulva, low temperature stress, transcriptome, hormone, soluble sugar

CLC Number: