Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (1): 131-139.doi: 10.7525/j.issn.1673-5102.2023.01.014

• Molecular biology • Previous Articles     Next Articles

Cloning and Expression Analysis of MibZIP1 from Macadamia integrifolia

Haiyun SONG1, Tao ZHANG1, Peng HE1, Shufang ZHENG1, Lifeng WANG2, Wenlin WANG1()   

  1. 1.Guangxi South Subtropical Agricultural Science Research Institute,Longzhou 532400
    2.Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
  • Received:2021-11-09 Online:2023-01-20 Published:2022-12-23
  • Contact: Wenlin WANG E-mail:wwlin3949@163.com
  • About author:SONG Haiyun(1986—),female,agronomist,mainly engaged in the evaluation and utilization of tropical and subtropical fruit germplasm resources.
  • Supported by:
    Youth science fund project of Guangxi(2019GXNSFBA185011);Science and Technology Project of Chongzuo City(Chongkegong(2020001);Chongke 20210710;Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(Guinongke 2021JM32,Guinongke 2021JM127,Guinongke 2020YM48,Guinongke 2020YM141);Basic business project of Guangxi Academy of Agricultural Sciences(Guinongke 2020YM46);Stable Support Project of Guangxi Academy of Agricultural Sciences(Guinongke 2021YT156)


In order to clarify the potential functions of bZIP transcription factor family members in Macadamia integrifolia under stress response, MibZIP1 was cloned from ‘Guire 1’ fruit. Combined with the analysis of transcription factor structure and expression patterns under different treatments, it was found that the full length of MibZIP1 was 1 157 bp, and the ORF was 927 bp, and encoded 308 aa,and had a typical bZIP Superfamily domain. Phylogenetic analysis showed that MibZIP1, TsbZIP60 and NnbZIP60 were closely related. The results of tissue expression analysis showed that MibZIP1 expression was the lowest in leaves of ‘Guire 1’ and the highest in branches of ‘695’. The expression of MibZIP1 was significantly up-regulated in ‘Guire 1’ leaves treated by zeatin, salicylic acid, ethephon and abscisic acid respectively, but was unchanged treated by gibberellin and hydrogen peroxide respectively. It was speculated that MibZIP1 was related to the stress tolerance of M. integrifolia, which provided theoretical guidance for the development of new cultivation and hormone regulation techniques.

Key words: Macadamia integrifolia, MibZIP1, gene cloning, expression, hormone

CLC Number: