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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (6): 1005-1013.doi: 10.7525/j.issn.1673-5102.2022.06.010

• Molecular biology • Previous Articles     Next Articles

Cloning and Drought Tolerance Expression Analysis of PtrWRKY51 Gene in Populus trichocarpa

Anying HUANG1,2, Dean XIA3, Yang ZHANG3, Dongchen NA4, Qing YAN4, Zhigang WEI1()   

  1. 1.Research Center for Saline-alkali Land,National Forestry and Grassland Administration,Beijing 100091
    2.China Eucalypt Research Centre,Chinese Academy of Forestry(CAF),Zhanjiang 524022
    3.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
    4.College of Life Science,Shanxi Normal University,Linfen 041000
  • Received:2021-10-20 Online:2022-11-20 Published:2022-11-22
  • Contact: Zhigang WEI E-mail:zhigangwei1973@163.com
  • About author:Huang Anying(1992—),female,master,mainly engaged in forest tree genetics and breeding research.
  • Supported by:
    Fundamental Research Funds of Chinese Academy of Forestry(CAFYBB2020ZA005);National Natural Science Foundation of China(31770640)


In order to clarify the function of PtrWRKY51 gene of Populus trichocarpa, the CDS of PtrWRKY51 gene was cloned using P. trichocarpa from Nisqually-1 strain as material. The function of PtrWRKY51 gene was studied by bioinformatics analysis, and assayed by yeast self-activation verification, and subcellular localization and simulated drought stress by real-time qPCR. The results showed that the CDS of PtrWRKY51 was 579 bp and encoded 192 aa. Bioinformatics analysis and subcellular localization experiments showed that PtrWRKY51 was a non-transmembrane alkaline unstable hydrophilic protein, located in the nucleus and contained a conserved domain unique to WRKY family, it was a WRKY transcription factor classified to class IIc. The yeast self-activation verification experiment showed that PtrWRKY51 gene could self-activation activity. qRT-PCR showed that the expression of this gene was significantly induced by 8% PEG6000 and reached the highest expression in stem and leaf after 12 h stress, while the roots appeared after 24 h stress. This study could provide reference for further study on stress tolerance and biological function of PtrWRKY51.

Key words: Populus trichocarpa, PtrWRKY51, bioinformatics, yeast self-activation, subcellular localization, drought tolerance expression analysis

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