Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (6): 1005-1013.doi: 10.7525/j.issn.1673-5102.2022.06.010
• Molecular biology • Previous Articles Next Articles
Anying HUANG1,2, Dean XIA3, Yang ZHANG3, Dongchen NA4, Qing YAN4, Zhigang WEI1()
Received:
2021-10-20
Online:
2022-11-20
Published:
2022-11-22
Contact:
Zhigang WEI
E-mail:zhigangwei1973@163.com
About author:
Huang Anying(1992—),female,master,mainly engaged in forest tree genetics and breeding research.
Supported by:
CLC Number:
Anying HUANG, Dean XIA, Yang ZHANG, Dongchen NA, Qing YAN, Zhigang WEI. Cloning and Drought Tolerance Expression Analysis of PtrWRKY51 Gene in Populus trichocarpa[J]. Bulletin of Botanical Research, 2022, 42(6): 1005-1013.
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URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.06.010
Table 1
PCR primer sequences
引物名称 Primer | 引物序列 Primer sequence(5′→3′) |
---|---|
PtrWRKY51-F | ATGGAGTACTATCCTCAGAACCCTA |
PtrWRKY51-R | CTAGGAAAAGGAAGAAGAGTGGAG |
PtrActin-F | AGGCAGGTTTCGCAGGAGATGA |
PtrActin-R | TCCATCACCAGAATCCAGCACA |
pGBKT7-PtrWRKY51-F | CATATGGCCATGGAGGCCGAATTCATGGAGTACTATCCTCAGAACCCTA |
pGBKT7-PtrWRKY51-R | GCCGCTGCAGGTCGACGGATCCCTAGGAAAAGGAAGAAGAGTGGAG |
pGBKT7-F | TCATCGGAAGAGAGTAGT |
pGBKT7-R | TTCGTTTTAAAACCTAAGAGTC |
PBI121-GFP-PtrWRKY51-F | ACGAGCTGTACAAGGGATCCATGGAGTACTATCCTCAGAACCCTA |
PBI121-GFP-PtrWRKY51-R | CTCTAGACTCACCTAGGATCCCTAGGAAAAGGAAGAAGAGTGGAG |
DL-PtrWRKY51-F | AGTTTCAGCCGTCGGATTATC |
DL-PtrWRKY51-R | CTGACTTCTTTCCATACTTCCTCC |
Fig.3
Subcellular Localization of PtrWRKY51 genePBI121-GFP-PtrWRKY51.Localization of fusion proteins and GFP proteins in tobacco leaf cells;GFP.Green fluorescent signal;DAPI. 4',6-diamidine-2-phenylindole,a class of fluorescent dyes strongly bound to DNA;Light.Bright field signal;Merge.Merges signals
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