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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (5): 802-810.doi: 10.7525/j.issn.1673-5102.2022.05.011

• Molecular biology • Previous Articles     Next Articles

Structural and Gene Expression Analysis of a DUF1262 Domain Protein in Latex from Hevea brasiliensis

Longjun DAI1, Mingyang LIU1,2, Jianghua YANG1, Kai ZHOU1,2, Bingbing GUO1, Hong YANG1, Lifeng WANG1,2()   

  1. 1.Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs;State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops;Danzhou Investigation & Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs;Rubber Research Institute,Key Laboratory of Materials Engineering for High Performance Natural Rubber,Hainan Province,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
    2.School of Plant Protection,Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests(Hainan University),Ministry of Education,Hainan University,Haikou 570228
  • Received:2022-06-20 Online:2022-09-20 Published:2022-09-15
  • Contact: Lifeng WANG E-mail:lfwang@catas.cn
  • About author:DAI Longjun(1976—),male,associate researcher,engaged in research on rubber tree biochemistry and molecular biology.
  • Supported by:
    National Natural Science Foundation of China(31870577)


To reveal the structure and function of natural rubber biosynthase interacting proteins in natural rubber biosynthesis, the total protein of latex rubber particles of Hevea brasiliensis was used as material, and an unknown functional protein containing DUF1262 domain was captured by immunoprecipitation assay using an antibody targeting CPT, a key enzyme for natural rubber biosynthesis. Bioinformatics analysis showed that there were 50 gene sequences encoding DUF1262 domain proteins in the rubber tree genome, and protein interaction network analysis suggested that DUF1262 domain proteins might be involved in signal transduction and transcriptional regulation. Fluorescence quantitative PCR results showed that the transcription of the gene encoding this protein was widely distributed in roots, leaves, flowers, branches, latex and other tissues respectively, andthe expression was low in latex and but high in bark. Salicylic acid, abscisic acid, hydrogen peroxide and drought treatment could enhance the transcription level of this gene in leaves respectively. This study demonstrated that the DUF1262 protein was involved in stress response and other physiological processes of rubber tree, providing new clues for revealing the regulation mechanism of latex biosynthesis.

Key words: Hevea brasiliensis, DUF1262 domain, gene expression, natural rubber biosynthesis

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