Effects of Tapping Panel Dryness on Mitochondrial Ultrastructure and ROS Metabolism in Barks of Rubber Tree (Hevea brasiliensis)

doi:10.7525/j.issn.1673-5102.2023.01.008

Abstract

Abstract:

In order to elucidate the mechanism of tapping panel dryness（TPD） and effectively control it， the ultrastructure and the expression patterns of reactive oxygen species（ROS）-related genes in barks from healthy（H） and different degrees of TPD trees were studied in this study. The results showed that with the development of TPD， aberrant mitochondrial ultrastructure changed including the loss of the matrix， swelling of cristae， and the cavitation of the inner cavity were observed in barks. The results of qRT-PCR experiments showed that the expression levels of HbPOD2 and HbPOD3 in TPD trees were higher than those in healthy trees， indicating the possibility of these genes being used as “marker” genes to monitor the tapping intensity， the stimulation intensity， and the occurrence of TPD. The gene HbCAT was also down-regulated in TPD trees， indicating that the balance between ROS generation and scavenging may be a key factor in the occurrence of TPD in rubber tree. The expressions of HbGST1， HbGST2， and HbPPO in TPD trees were higher than those in healthy trees， indicating these genes may be associated with in-situ coagulation of latex during TPD occurrence. This study reveals the changes of ultrastructure and the expression patterns of ROS-related genes in rubber tree barks during TPD occurrence. These findings may provide a new perspective for elucidating the mechanism of TPD in rubber tree， and provid theoretical guidance for developing methods for monitoring tapping intensity and stimulation intensity and for developing TPD-related “marker” genes in rubber trees.

Key words: Hevea brasiliensis, tapping panel dryness, ultrastructure, reactive oxygen species, gene expression

CLC Number:

S794.1

Hong YANG, Lifeng WANG, Longjun DAI, Bingbing GUO. Effects of Tapping Panel Dryness on Mitochondrial Ultrastructure and ROS Metabolism in Barks of Rubber Tree (Hevea brasiliensis)[J]. Bulletin of Botanical Research, 2023, 43(1): 69-75.

Figures/Tables 4

Table 1

Fig.1

Fig.2

Fig.3

References 30

1	曾霞，黄华孙.我国天然橡胶技术发展现状与展望［J］.中国热带农业，2021（1）：25-30.
	ZENG X， HUANG H S.Development and prospects of natural rubber technology in China ［J］.China Tropical Agriculture，2021（1）：25-30.
2	何振革，蒋菊生，郑定华.橡胶种植密度、形式与生长及产量效益的关系［J］.现代农业科技，2008（7）：5-7，10.
	HE Z G， JIANG J S， ZHENG D H.Relationship between planting density，planting pattern and growth，yield in rubber tree［J］.Modern Agricultural Science and Technology，2008（7）：5-7，10.
3	王真辉，袁坤，陈邦乾，等.中国主要植胶区橡胶树死皮发生现状及田间分布形式研究［J］.热带农业科学，2014，34（11）：66-70，74.
	WANG Z H， YUAN K， CHEN B Q，et al.Occurring and field distribution of tapping panel dryness in Hevea brasiliensis in the main planting areas of China［J］.Chinese Journal of Tropical Agriculture，2014，34（11）：66-70，74.
4	邹智，杨礼富，王真辉，等.橡胶树“死皮”及其防控策略探讨［J］.生物技术通报，2012（9）：8-15.
	ZOU Z， YANG L F， WANG Z H，et al.Strategies on prevention and treatment of tapping panel dryness syndrome of rubber（Hevea brasiliensis Muell.Arg.）［J］.Biotechnology Bulletin，2012（9）：8-15.
5	吴继林，谭海燕，郝秉中.乙烯利过度刺激采胶诱导巴西橡胶树割面干涸病的研究［J］.热带作物学报，2008，29（1）：1-9.
	WU J L， TAN H Y， HAO B Z.Aspects of the development of tapping panel dryness induced by overstimulation with ethrel in Hevea brasiliensis ［J］.Chinese Journal of Tropical Crops，2008，29（1）：1-9.
6	VENKATACHALAM P， THULASEEDHARAN A， RAGHOTHAMA K.Identification of expression profiles of tapping panel dryness （TPD） associated genes from the latex of rubber tree （Hevea brasiliensis Muell.Arg.）［J］.Planta，2007，226（2）：499-515.
7	VENKATACHALAM P， THULASEEDHARAN A， RAGHOTHAMA K.Molecular identification and characterization of a gene associated with the onset of tapping panel dryness （TPD） syndrome in rubber tree （Hevea brasiliensis Muell.） by mRNA differential display［J］.Molecular Biotechnology，2009，41（1）：42-52.
8	DENG Z， ZHAO M M， LIU H，et al.Molecular cloning，expression profiles and characterization of a glutathione reductase in Hevea brasiliensis ［J］.Plant Physiology and Biochemistry，2015，96：53-63.
9	LIU J P， ZHUANG Y F， GUO X L，et al.Molecular mechanism of ethylene stimulation of latex yield in rubber tree （Hevea brasiliensis） revealed by de novo sequencing and transcriptome analysis［J］.BMC Genomics，2016，17：257.
10	范思伟，杨少琼.强割和排胶过度引起的死皮是一种特殊的局部衰老病害［J］.热带作物学报，1995，16（2）：15-22.
	FAN S W， YANG S Q.Aspecial and localized senescence disease-bakk dryness due toover exploitation and over-drainage［J］.Chinese Journal of Tropical Crops，1995，16（2）：15-22.
11	NAVROT N， ROUHIER N， GELHAYE E，et al.Reactive oxygen species generation and antioxidant systems in plant mitochondria［J］.Physiologia Plantarum，2007，129（1）：185-195.
12	袁坤，杨礼富，陈帮乾，等.海南植胶区橡胶树死皮发生现状分析［J］.西北林学院学报，2016，31（1）：176-179.
	YUAN K， YANG L F， CHEN B Q，et al.Current situation of Hevea brasiliensis tapping panel drynes occurring in Hainan［J］.Journal of Northwest Forestry University，2016，31（1）：176-179.
13	郭秀丽，孙亮，胡义钰，等.巴西橡胶树不同死皮程度植株的胶乳生理参数分析［J］.南方农业学报，2016，47（9）：1553-1557.
	GUO X L， SUN L， HU Y Y，et al.Analysis on latex physiological parameters of Hevea brasiliensis plants at different tapping panel dryness extents［J］.Journal of Southern Agriculture，2016，47（9）：1553-1557.
14	LI D J， WANG X C， DENG Z，et al.Transcriptome analyses reveal molecular mechanism underlying tapping panel dryness of rubber tree （Hevea brasiliensis）［J］.Scientific Reports，2016，6：23540.
15	MONTORO P， WU S Y， FAVREAU B，et al.Transcriptome analysis in Hevea brasiliensis latex revealed changes in hormone signalling pathways during ethephon stimulation and consequent Tapping Panel Dryness［J］.Scientific Reports，2018，8（1）：8483.
16	LIU J P， XIA Z Q， TIAN X Y，et al.Transcriptome sequencing and analysis of rubber tree （Hevea brasiliensis Muell.） to discover putative genes associated with tapping panel dryness（TPD）［J］.BMC Genomics，2015，16（1）：398.
17	陈慕容，黄庆春，叶沙冰，等.“保01”防治橡胶树褐皮病及其作用机理的研究［J］.热带作物研究，1992，12（1）：30-37.
	CHEN M R， HUANG Q C， YE S B，et al.Research on "Bao 01" on the control of rubber tree brown bast and its mechanism of action［J］.Tropical Crops Research，1992，12（1）：30-37.
18	袁坤，谢贵水，杨礼富，等.不同药剂处理对橡胶树死皮和产量的影响［J］.西南农业学报，2013，26（4）：1524-1526.
	YUAN K， XIE G S， YANG L F，et al.Effect of different medicaments on tapping panel dryness（TPD） and yield in Hevea brasiliensis ［J］.Southwest China Journal of Agricultural Sciences，2013，26（4）：1524-1526.
19	TANG C R， YANG M， FANG Y J，et al.The rubber tree genome reveals new insights into rubber production and species adaptation［J］.Nature Plants，2016，2（6）：16073.
20	卢亚莉，张世鑫，王真辉，等.巴西橡胶树RY7-33-97不同级别死皮树皮结构分析［J］.热带作物学报，2021，42（7）：1918-1924.
	LU Y L， ZHANG S X， WANG Z H，et al.Bark structure of the clone ‘RY7-33-97’ with different level of tapping panel dryness（TPD） in rubber tree（Hevea brasiliensis Muell.Arg.）［J］.Chinese Journal of Tropical Crops，2021，42（7）：1918-1924.
21	VAN AKEN O.Mitochondrial redox systems as central hubs in plant metabolism and signaling ［J］.Plant Physiology，2021，186（1）：36-52.
22	ZHANG Y， LECLERCQ J， MONTORO P.Reactive oxygen species in Hevea brasiliensis latex and relevance to Tapping Panel Dryness［J］.Tree Physiology，2017，37（2）：261-269.
23	LI D J， DENG Z， CHEN C L，et al.Identification and characterization of genes associated with tapping panel dryness from Hevea brasiliensis latex using suppression subtractive hybridization［J］.BMC Plant Biology，2010，10：140.
24	许闻献，校现周.橡胶死皮树过氧化物酶同工酶和超氧化物歧化酶同工酶的研究［J］.热带作物学报，1988，9（1）：31-36.
	XU W X， XIAO X Z.Studies on peroxidase and superoxidase dismutase isozymes in dry rubber trees［J］.Chinese Journal of Tropical Crops，1988，9（1）：31-36.
25	SASAN M， MARYAM E， FATEME M，et al.Plant glutathione S-transferase classification，structure and evolution［J］.African Journal of Biotechnology，2011，10（42）：8160-8165.
26	FROVA C.The plant glutathione transferase gene family：genomic structure，functions，expression and evolution［J］.Physiologia Plantarum，2003，119（4）：469-479.
27	范玉洁，林飞鹏，安泽伟，等.一个橡胶树谷胱甘肽-S-转移酶基因的克隆和表达特性分析［J］.中国农业科学，2011，44（20）：4150-4158.
	FAN Y J， LIN F P， AN Z W，et al.Cloning and expression analysis of a glutathione-s-transferase gene in the latex of Hevea brasiliensis（para rubber tree）［J］.Scientia Agricultura Sinica，2011，44（20）：4150-4158.
28	史敏晶，邓顺楠，陈月异，等.巴西橡胶树胶乳黄色体中多酚氧化酶的活性及其橡胶粒子凝集作用的研究［J］.热带作物学报，2013，34（10）：1966-1971.
	SHI M J， DENG S N， CHEN Y Y，et al.The enzymatic activity and its effect on rubber particle aggregation of polyphenol oxidase from lutoid of latex in Hevea brasiliensis ［J］.Chinese Journal of Tropical Crops，2013，34（10）：1966-1971.
29	董守坤，马玉玲，李爽，等.干旱胁迫及复水对大豆抗坏血酸-谷胱甘肽循环的影响［J］.东北农业大学学报，2018，49（1）：10-18.
	DONG S K， MA Y L， LI S，et al.Effect of drought stress and re-watering on ascorbate-glutathionecycle of soybean［J］.Journal of Northeast Agricultural University，2018，49（1）：10-18.
30	柯笑楠，胡海超，黄霞，等.水稻抗坏血酸氧化酶基因家族生物信息学分析［J］.分子植物育种，2020，18（1）：11-19.
	KE X N， HU H C， HUANG X，et al.Bioinformatics analysis of ascorbic acid oxidase gene family in Oryza sativa L.［J］.Molecular Plant Breeding，2020，18（1）：11-19.

基因名称 Gene name	正向引物（5′→3′） Sense primer（5′→3′）	反向引物（5′→3′） Anti-sense primer（5′→3′）
HbPAO	GCAACCTGAAGCCCAACAATCA	ACATAACCGAGCCATCGCAGAA
HbCAT	TCTATTGTGGTGCCTGGTGTCT	GCCAAGGCGATACCTCTGAGT
HbAAO	GCTCATCATTCACTTGCTTCTTCTCC	AATTAGGCTCAGAGGACAGGAAGG
HbPPO	CATTGTGCTTACTGTGATGGTGCTTAT	GCTGGTGATTCTTGTTGCGATACTG
HbGST1	TGTTATGGAAGAAGTTGGTGGCATGA	AGCAGTGAGGTAGTTGACAATCAGATC
HbGST2	TGCTGTAGGATGGATCTCTTACTGGTT	GAAATTGCTGGAAATTGCTGGCTCTC
HbGST3	GAGAAGTTGTTGAATGGATGCAGAAGA	CGAGCAATCACCACACGCCTTA
HbGST4	CTTCGGTGTTGTTCTCCAGCAAGT	GAGCAACTGAGGTCGCCTTCTG
HbLOX1	GCCTGGTTGCGAGATGACGAATT	GCAGACTCCTGTGGACCGTAGAT
HbLOX2	TTCCTATGCCTCAAGTGATTCAAGTG	TTCCTCAAGACAACGAATGCTAACAG
HbPOD1	CAGGGAGGAGGGACAGCAAAGA	GTTCCTTCAGCGTCAATGCCAATG
HbPOD2	GTGATGCTGTAGACTTGAGTGATGGT	AGGCTTGGGAGGATGACGAGAC
HbPOD3	GCAGAGTGTCCTGGTGTGGTT	AGATTGTTCCATCTCTTCTTCCTGTTG
HbPOD4	ACTCAAGACAATAGCAAACCACCCAAA	GCAGCAGCAGCAAGACTGAAGA
HbPOD5	GTGGATGAGATGGTGACGCTGTC	GTGTAGAGACGGCTGGAGAAGGA
HbPOD6	GTGGATGAGATGGTGACGCTGTC	GTGTAGAGACGGCTGGAGAAGGA
HbPOD7	GAATTGGTGACGCTCTCTGGTGTT	TGGCTCTCAAGTAACTGGCATAGGT
Hb18S	GCTCGAAGACGATCAGATACC	TTCAGCCTTGCGACCATAC

[1]	Lei XU, Xiao XU, Qinsong LIU. Effects of Exogenous Salicylic Acid on Antioxidant System and Gene Expression of Davidia involucrata Seedlings under Salt Stress [J]. Bulletin of Botanical Research, 2023, 43(4): 572-581.
[2]	Mingyue YUAN, Tianzhong ZHOU, Ma YU, Bin HU, Xiangyu LONG, Hua CHEN. Cloning and Functional Analysis of Deubiquitinating Enzyme Gene UCHs from Hevea brasiliensis [J]. Bulletin of Botanical Research, 2023, 43(4): 622-630.
[3]	Mengshuo LI, Yingze LIU, Huan LU, Sheng QIANG. Photosynthetic Capacity Differentiation and Gene Transcription in Different Geographical Populations of Arabidopsis thaliana under Common Garden conditions [J]. Bulletin of Botanical Research, 2023, 43(1): 90-99.
[4]	Bi QIN, Mingyang LIU, Meng WANG, Lifeng WANG, Fei HUANG. Cloning and Expression Analysis of DELLA gene HbRGL1 from Hevea brasiliensis Müll. Arg [J]. Bulletin of Botanical Research, 2022, 42(6): 997-1004.
[5]	Longjun DAI, Mingyang LIU, Jianghua YANG, Kai ZHOU, Bingbing GUO, Hong YANG, Lifeng WANG. Structural and Gene Expression Analysis of a DUF1262 Domain Protein in Latex from Hevea brasiliensis [J]. Bulletin of Botanical Research, 2022, 42(5): 802-810.
[6]	Sisi CHEN, Muhong XIE, Maokai CUI, Wenkai LI, Zhenggang XU, Caixia JIA, Guiyan YANG. Identification of Broussonetia papyrifera Transcription Factor BpbZIP1 and Analysis of Its Response to Cadmium Stress [J]. Bulletin of Botanical Research, 2022, 42(3): 394-402.
[7]	Jianxin LIU, Ruirui LIU, Xiuli LIU, Haiyan JIA, Ting BU, Na LI. Effects of Spraying NaHS at Different Growth Stages on H₂S Production and Reactive Oxygen Species Metabolism of Naked Oat Leaves under Saline-Alkali Stress [J]. Bulletin of Botanical Research, 2022, 42(3): 455-465.
[8]	Kaiheng Ma, Jianing He, Muhong Xie, Bin Ren, Qianxue Huang, Guiyan Yang. Cloning and Disease Resistance Response Potential of Walnut JrNPR1 Gene [J]. Bulletin of Botanical Research, 2022, 42(1): 39-46.
[9]	Xiu-Yun YUAN, Shen-Ping XU, Yi-Ran ZHOU, Xi-Meng WNG, Bo CUI. Shading Effect on Morphology and Leaf Structure of Bletilla striata [J]. Bulletin of Botanical Research, 2021, 41(6): 974-981.
[10]	Hua-Feng CHEN, Song-Le FAN, Li-Feng WANG. Structural and Functional Analysis of Gibberellin Signaling Transduction Pathway Gene HbRGA1 in Rubber Tree [J]. Bulletin of Botanical Research, 2021, 41(4): 531-539.
[11]	SUN Wang-Wang, MENG Xian-Min, XU Xiu-Yuan, WU Min-Hua, QIN Meng-Yang, LIN Meng-Meng, ZHANG Xin-Yan, YUE Guo-Zhong. Contents of Pigments and Anatomical Structure in the Leaves of Forsythia koreana ‘Sun Gold’ [J]. Bulletin of Botanical Research, 2020, 40(3): 321-329.
[12]	LIU Yue, ZHAO Kai, Lü Guan-Bin, JIANG Ting-Bo, ZHOU Bo-Ru. Transcription Factor Gene ERF11 Response to Osmotic Stress in Populus simonii×P.nigra Poplar [J]. Bulletin of Botanical Research, 2020, 40(3): 433-440.
[13]	HE Hao, ZHU Guo-Qing, CHEN Shi-Ya, XU Chang, JIN Shu-Mei. Cloning of LpPEX7 Gene from Lilium pumilum and Its Expression Characteristics under Salt Stress [J]. Bulletin of Botanical Research, 2020, 40(2): 274-283.
[14]	LI Dong-Lin, JIN Ya-Qin, CUI Meng-Fan, HUANG Lin-Xi, PEI Wen-Hui. Effects of Shading on Physiological Characteristics and Ultrastructure of Mesophyll Cell of Emmenopterys henryi Leaves [J]. Bulletin of Botanical Research, 2020, 40(1): 29-40.
[15]	FAN Song-Le, WANG Ji-Kun, XIE Gui-Shui, WANG Meng, WANG Li-Feng. Structural and Functional Analysis of R2R3 MYB Transcription Factor HbMYB88 [J]. Bulletin of Botanical Research, 2020, 40(1): 106-116.