Effects of Exogenous Hormones Soaking and Osmotic Treatment on Thermal Dormancy of Seeds of Fraxinus mandshurica

doi:10.7525/j.issn.1673-5102.2023.03.006

Abstract

Abstract:

To explore the effects of exogenous hormones soaking combined with polyethylene glycol 6000 （PEG） osmotic treatment on the thermal dormancy and related physiological changes in seeds of Fraxinus mandshurica， non-dormant F. mandshurica seeds were used as materials and then soaked with 1 mmol·L^-1 gibberellin（GA₄₊₇）， ethylene（ETH） and cytokinins（6-BA） for 24 h respectively， and the germination of seeds cultured in different mass fractions（0， 5%， 25%， 50%） PEG solution at the induction thermal dormancy temperature（25 ℃）for 7 d and then at 10 ℃ was assayed. On this basis， the treatments with significant results were selected to determine the changes in seeds endogenous hormones content and cell wall degrading enzyme activity. The results showed that： compared with hormone treatment alone， GA₄₊₇ combined with different mass fraction PEG had no significant effect on germination ability. With the increase of PEG mass fraction， the germination ability of 6-BA combined with PEG treatment gradually increased and then tend to stabilized， when combined with 25% PEG， the germination percentage and germination index respectively increased 69.31% and 1.04， and the germination time was shortened by 7.8 d. The seeds germination ability treated ETH combined with PEG gradually increased with the increase of PEG mass fraction， when combined with 50% PEG， the germination percentage and germination index respectively increased 43.43% and 0.76. Hormone combined with osmotic treatment significantly increased endosperm cellulase activity， but the GA and ABA contents in embryo and endosperm were not significantly regularity. In conclusion， exogenous hormone combined with osmotic treatment could reduce the thermal dormancy of F. mandshurica seeds， and the effect was different depending on the hormone species.

Key words: Fraxinus mandshurica, seed germination, secondary dormancy, exogenous hormone, osmotic treatment, endogenous hormone

CLC Number:

S792

Chunjing JIAO, Mingyue LI, Peng ZHANG. Effects of Exogenous Hormones Soaking and Osmotic Treatment on Thermal Dormancy of Seeds of Fraxinus mandshurica[J]. Bulletin of Botanical Research, 2023, 43(3): 370-378.

Figures/Tables 5

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PEG质量分数 PEG mass fraction /%	激素 Hormones	发芽率 Germination percentage /%	发芽指数 Germination index	平均发芽时间 Germination time /d
0	对照（水）	6.13±3.03g	0.14±0.06h	13.13±0.89f
	GA₄₊₇	82.51±2.96a	1.34±0.13ab	16.91±0.85de
	6-BA	21.98±2.29f	0.21±0.02gh	25.88±0.96a
	ETH	9.00±3.00fg	0.17±0.05gh	14.35±1.63ef
5	对照	13.70±3.52fg	0.22±0.06gh	15.93±1.02def
	GA₄₊₇	75.27±2.77ab	1.09±0.76abcd	18.42±0.98cd
	6-BA	68.05±5.15bc	0.78±0.05cdef	22.04±0.38b
	ETH	15.29±4.57fg	0.28±0.08gh	13.48±0.58f
25	对照	32.83±9.80de	0.52±0.18fg	16.39±1.29de
	GA₄₊₇	88.56±4.96a	1.43±0.14a	16.65±0.54de
	6-BA	91.29±1.85a	1.25±0.09abc	18.08±0.73cd
	ETH	44.96±2.52d	0.71±0.20ef	15.73±0.35def
50	对照	59.39±8.46cd	0.94±0.16cdef	16.14±0.35de
	GA₄₊₇	83.42±1.31ab	1.06±0.03bcde	19.93±0.49bc
	6-BA	89.12±1.58ab	1.24±0.04abc	17.99±0.38cd
	ETH	52.42±8.30cd	0.93±0.15cde	14.26±0.28ef

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