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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (3): 370-378.doi: 10.7525/j.issn.1673-5102.2023.03.006

• Physiology and Ecology • Previous Articles     Next Articles

Effects of Exogenous Hormones Soaking and Osmotic Treatment on Thermal Dormancy of Seeds of Fraxinus mandshurica

Chunjing JIAO, Mingyue LI, Peng ZHANG()   

  1. Key Laboratory of Sustainable Forest Ecosystem Management Ministry of Education,School of Forestry,Northeast Forestry University,Harbin 150040
  • Received:2023-03-10 Online:2023-05-20 Published:2023-05-11
  • Contact: Peng ZHANG E-mail:zhangpeng@nefu.edu.cn
  • About author:JIAO Chunjing(1998—),female,master student,mainly engaged in the research of silviculture.
  • Supported by:
    National Natural Science Foundation of China(31870615)


To explore the effects of exogenous hormones soaking combined with polyethylene glycol 6000 (PEG) osmotic treatment on the thermal dormancy and related physiological changes in seeds of Fraxinus mandshurica, non-dormant F. mandshurica seeds were used as materials and then soaked with 1 mmol·L-1 gibberellin(GA4+7), ethylene(ETH) and cytokinins(6-BA) for 24 h respectively, and the germination of seeds cultured in different mass fractions(0, 5%, 25%, 50%) PEG solution at the induction thermal dormancy temperature(25 ℃)for 7 d and then at 10 ℃ was assayed. On this basis, the treatments with significant results were selected to determine the changes in seeds endogenous hormones content and cell wall degrading enzyme activity. The results showed that: compared with hormone treatment alone, GA4+7 combined with different mass fraction PEG had no significant effect on germination ability. With the increase of PEG mass fraction, the germination ability of 6-BA combined with PEG treatment gradually increased and then tend to stabilized, when combined with 25% PEG, the germination percentage and germination index respectively increased 69.31% and 1.04, and the germination time was shortened by 7.8 d. The seeds germination ability treated ETH combined with PEG gradually increased with the increase of PEG mass fraction, when combined with 50% PEG, the germination percentage and germination index respectively increased 43.43% and 0.76. Hormone combined with osmotic treatment significantly increased endosperm cellulase activity, but the GA and ABA contents in embryo and endosperm were not significantly regularity. In conclusion, exogenous hormone combined with osmotic treatment could reduce the thermal dormancy of F. mandshurica seeds, and the effect was different depending on the hormone species.

Key words: Fraxinus mandshurica, seed germination, secondary dormancy, exogenous hormone, osmotic treatment, endogenous hormone

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