Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (6): 1023-1032.doi: 10.7525/j.issn.1673-5102.2022.06.012

• Molecular biology • Previous Articles     Next Articles

Stress Tolerance Functional Analysis of the High Expression Heat Shock Protein HbHSP90.4 Gene from the Latex of Hevea brasiliensis

Huafeng CHEN1, Longjun DAI2, Mingyang LIU2, Bingbing GUO2, Hong YANG2, Lifeng WANG2()   

  1. 1.Northeast Forestry University,Harbin 150040
    2.Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,P. R. China;State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops;Danzhou Investigation & Experiment Station of Tropical Crops,Ministry of Agriculture and Rural Affairs,P. R. China;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101
  • Received:2022-06-17 Online:2022-11-20 Published:2022-11-22
  • Contact: Lifeng WANG E-mail:lfwang@catas.cn
  • About author:CHEN Huafeng(1978—),male,postgraduate,major in plant metabolism and physiological ecology.
  • Supported by:
    National Natural Science Foundation of China(31870577)


In order to analyze the role of Heat Shock Protein 90(HSP90) in stress and phytohormone signaling transduction in rubber tree, the full-length cDNA sequence of the HbHSP90.4 gene was cloned from rubber tree Reyan73397 latex by PCR, and HbHSP90.4 gene contained a 2 451 bp open reading frame(ORF) and encoded 816 amino acids. The results of bioinformatics analysis showed that HbHSP90.4 contained the HSP90 superfamily and HATPase superfamily domains, which was a member of the HSP90 family. Phylogenetic analysis revealed that this protein was closely related to cassava MeHSP90. Subcellular localization predictions showed that the HbHSP90.4 gene was localized in endoplasmic reticulum. The qRT-PCR results showed that the HbHSP90.4 gene was mainly expressed in rubber latex. Drought, cold, powdery mildew infection, H2O2 and MeJA treatments promoted the up-regulation of the HbHSP90.4 gene in latex respectively, while its expression was significantly down-regulated in ETH, SA and ABA treatments respectively. The plant expression vector HbHSP90.4-mScarlet was also constructed to prepare the material for further transgenic plants. This study laid a solid foundation for the elucidation of the molecular regulation of the latex HbHSP90.4 gene in response to rubber tree stress and the phytohormone signaling pathway.

Key words: Rubber tree, HbHSP90.4, bioinformatics, expression analysis, expression vector construction

CLC Number: