Cloning and Expression Analysis of MibZIP1 from Macadamia integrifolia

doi:10.7525/j.issn.1673-5102.2023.01.014

Abstract

Abstract:

In order to clarify the potential functions of bZIP transcription factor family members in Macadamia integrifolia under stress response， MibZIP1 was cloned from ‘Guire 1’ fruit. Combined with the analysis of transcription factor structure and expression patterns under different treatments， it was found that the full length of MibZIP1 was 1 157 bp， and the ORF was 927 bp， and encoded 308 aa，and had a typical bZIP Superfamily domain. Phylogenetic analysis showed that MibZIP1， TsbZIP60 and NnbZIP60 were closely related. The results of tissue expression analysis showed that MibZIP1 expression was the lowest in leaves of ‘Guire 1’ and the highest in branches of ‘695’. The expression of MibZIP1 was significantly up-regulated in ‘Guire 1’ leaves treated by zeatin， salicylic acid， ethephon and abscisic acid respectively， but was unchanged treated by gibberellin and hydrogen peroxide respectively. It was speculated that MibZIP1 was related to the stress tolerance of M. integrifolia， which provided theoretical guidance for the development of new cultivation and hormone regulation techniques.

Key words: Macadamia integrifolia, MibZIP1, gene cloning, expression, hormone

CLC Number:

S664.9

Haiyun SONG, Tao ZHANG, Peng HE, Shufang ZHENG, Lifeng WANG, Wenlin WANG. Cloning and Expression Analysis of MibZIP1 from Macadamia integrifolia[J]. Bulletin of Botanical Research, 2023, 43(1): 131-139.

Figures/Tables 6

Table 1

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引物名称 Primer-ID	引物序列 Sequence（5′→3′）
MibZIP1 full length 1	TCATCCCAATTCTCACTCCC
MibZIP1 full length 2	CCAATCTCCATGCTCCACTAG
MibZIP1 QF	CCATCGTCAGTGAGCCATC
MibZIP1 QR	ACGCAGGACTCCGTATCG
MiACTIN QF	TCTTCATTGCCTGCACTCCAGA
MiACTIN QR	TTCCACCTGAATGCCGTCTAGC

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