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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (2): 283-290.doi: 10.7525/j.issn.1673-5102.2016.02.018

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Construction of Plant Expression Vector and Genetic Transformation Analysis of Arabidopsis thaliana CYCD2;1 Gene in Nicotiana tabacum

DAI Li-Juan;ZHENG Tang-Chun;LIU Cai-Xia;LI Kai-Long;QU Guan-Zheng*   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Online:2016-03-15 Published:2016-05-20

Abstract: Cell division is one of the basic characteristics of organisms, which plays an extremely important role in the plant growth and development. CYCD2;1, as a regulatory factor, has been identified in regulation of cell cycle. In our study, CYCD2;1 was amplified from Arabidopsis inflorescences cDNA by PCR. Plant overexpression vector (pROKII-AtCYCD2;1) was constructed and transformed into wild-type tobacco by Agrobacterium-mediated leaf disc transformation. The transgenic plants were confirmed by PCR, and the result showed AtCYCD2;1 gene was integrated into tobacco genome. The result of qRT-PCR detection of T2 generation plants showed the mRNA of AtCYCD2;1 was transcribed in transgenic tobaccos. There were significant differences in the flower between the transgenic and wild type plant, such as the width of the corolla, the lengths of the petal and sepal, and increased volume of seed pod, which suggesting overexpression of AtCYCD2;1 has an obvious influence on the development of flowers.

Key words: AtCYCD2, 1, cyclin, genetic transformation, Nicotiana tabacum