Cloning and Osmotic Stress Response Analysis of ThGRF2 from Tamarix hispida

doi:10.7525/j.issn.1673-5102.2022.06.014

Abstract

Abstract:

14-3-3 protein，also called general regulatory factors（GRF），is a type of serine and threonine phosphorylation binding protein， which participates in the regulation of series of physiological processes， such as intracellular basis， signal transduction， plant growth and development， environmental stress response， by interacting with other transcription factors or signaling proteins. In this study， the ThGRF2 gene that was differentially expressed under drought stress and cloned from the drought transcriptome of Tamarix hispida. The CDS fragment of ThGRF2 gene was 786 bp， encoded 261 amino acids. The relative molecular weight was 29.40 kDa， and the theoretical isoelectric point was 4.76. The overexpression vector pROKII-ThGRF2 was constructed， and transformed into T. hispida. The physiological index results showed that ThGRF2 overexpression increased the chlorophyll content， SOD and POD activities， and reduced malondialdehyde content（MDA）， electrical conductivity（EL） and water loss rate under osmotic stress， indicated that ThGRF2 gene played an important role in the response to osmotic stress of T.hispida. It laid a foundation for further exploration of the abiotic stress tolerance function of ThGRF2 gene.

Key words: Tamarix hispida, 14-3-3 protein, osmotic stress, physiological indicators

CLC Number:

Q943.2

Jing WU, Yuanyuan WANG, Danni WANG, Baichao LIU, Zhongyuan LIU. Cloning and Osmotic Stress Response Analysis of ThGRF2 from Tamarix hispida[J]. Bulletin of Botanical Research, 2022, 42(6): 1044-1051.

Figures/Tables 7

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基因命名 Gene name	引物序列 Primers sequences（5′—3′）
ThGRF-F	GCAAGATCTGCAACGGGAT
ThGRF-R	ACACGGAGAAGTTGAGCG
β-Actin-F	AAACAATGGCTGATGCTG
β-Actin-R	ACAATACCGTGCTCAATAGG
α-tubulin-F	CACCCACCGTTGTTCCAG
α-tubulin-R	ACCGTCGTCATCTTCACC
β-tubulin-F	GGAAGCCATAGAAAGACC
β-tubulin-R	CAACAAATGTGGGATGCT

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