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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (2): 291-296.doi: 10.7525/j.issn.1673-5102.2016.02.019

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Characteristic of Molecular Evolution of Notopterygium incisum Based on nrDNAITS and cpDNA rpl20-rps12 Sequence Analysis

YANG Lu-Cun1,2;LIU He-Chun1,3;ZHOU Xue-Li4;XU Wen-Hua1,2;ZHOU Guo-Ying1,2*   

  1. 1.Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008;
    2.Key Laboratory of Tibetan Medicine Research,Chinese Academy of Sciences,Xining 810008;
    3.University of Chinese Academy of Sciences,Beijing 100049;
    4.Tiebujia Grassland Improvement Experiment Station,Xining 810008
  • Online:2016-03-15 Published:2016-05-20

Abstract: We analyzed the differences between the nrDNAITS and cpDNA rpl20-rps12 sequences in Notopterygium incisum by PCR direct sequencing. Total DNA was extracted from silica-dried leaves of N.incisum using modified CTAB method. With the extracted DNA as template, nrDNA ITS and cpDNA rpl20-rps12 regions were amplified, then purified and sequenced. The length of nrDNA ITS sequence of N.incisum was 635 bp, of which 17 were variable sites with a percentage of 2.68%, the(G+C) content was 57.83%. The length of cpDNA rpl20-rps12 sequence of N.incisum was 767 bp, of which 35 was variable site with a percentage of 4.56%, the(G+C) content was 33.06%. The nrDNAITS region of N.incisum was more conserved and evolved more slowly than the cpDNA rpl20-rps12sequence. The present distribution range of N.incisum experienced range expansion by the haplotype analysis of this species, which consisted with the conclusion resulting from cpDNA genome. Therefore, the nrDNA ITS sequence of rpl20-rps12 was fit to the phylogeographic study of this species.

Key words: Notopterygium incisum, ITSsequence, rpl20-rps12 sequence