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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (2): 300-310.doi: 10.7525/j.issn.1673-5102.2023.02.015

• Molecular biology • Previous Articles     Next Articles

Response Analysis of Transcription Factor PsnbHLH162 Gene in Populus simonii × P. nigra under Salt Stress and Low Temperature Stress

Senyao LIU, Fenglin JIA, Qing GUO, Gaofeng FAN, Boru ZHOU, Tingbo JIANG()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2022-04-19 Online:2023-03-20 Published:2023-03-07
  • Contact: Tingbo JIANG E-mail:tbjiang@yahoo.com
  • About author:LIU Senyao(1997—),male,graduate student,specialized in research on forest genetics and breeding.
  • Supported by:
    Application Technology Research and Development Program of Heilongjiang Province(GA20B401)


To reveal the role of transcription factor PsnbHLH162 of Populus simonii × P. nigra under abiotic stresses, and to explore the signal transduction process of genes in plants, the signal transduction process of this gene in plants is explored to provide the theoretical basis for the acquisition of excellent stress-resistant tree species in the future. In this experiment, PsnbHLH162 gene was cloned and obtained from P. simonii × P. nigra as material, and the target gene and promoter were analyzed by bioinformatics; and the function of genes in response to abiotic stresses was analyzed by fluorescence quantitative PCR. The results showed that the cDNA of PsnbHLH162 gene was 537 bp, and the N-terminal of the gene contained a highly conserved HLH domain, and the encoded protein might be a stable hydrophilic protein without trans-membrane region, localized in the nucleus and had no transcriptional activation activity. The promoter region contained a variety of ABA-responsive, auxin-responsive, light-responsive and circadian elements, confirming that this gene participated in abiotic stress response. The results of fluorescence quantitative PCR showed that under salt stress, the gene expression in root tissue was the highest compared with stem and leaf tissue; under low temperature stress, gene expression in stem tissue was the highest compared with leaf and root tissue. It was found that the expression of PsnbHLH162 could be induced by salt and low temperature in plants.

Key words: Populus simonii × P. nigra, PsnbHLH 162, bioinformatics, low temperature stress, salt stress

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