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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (4): 414-421.doi: 10.7525/j.issn.1673-5102.2011.04.007

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Isolation and Analysis of Day-night Differential Expressed ESTs from Paraquilegia microphylla

LIU De-Tuan;LI Wan-Sha;ZHA Hong-Guang;YANG Yong-Ping;HU Xiang-Yang*   

  1. 1.Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204;2.Graduate University of Chinese Academy of Sciences,Beijing 100049
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20
  • Contact: HU Xiang-Yang1
  • Supported by:

Abstract: Suppression subtractive hybridization (SSH) based on magnetic beads technology was used to screen day-night differential expression genes of Paraquilegia microphylla living on the scree of the Baima snow mountain of Tibetan plateau. The daytime sample was used as tester and nighttime sample as driver for further analysis. Finally, 96 differential ESTs between two samples were sequenced. Sequences were analyzed with BlastX, Blast2go and KEGG. The results indicated that most differential expression proteins are related to material and energy metabolism, biosynthetic process, have nucleic acid binding, protein binding and transferase activity; and a few are responsive to stress, etc. These results will provide some prerequisites for further researches on alpine plants under harsh environments.

Key words: alpine plant, stress, suppression subtractive hybridization(SSH)

CLC Number: