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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (4): 481-492.doi: 10.7525/j.issn.1673-5102.2023.04.001

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Application Progress of RNA-Seq Technology in Rare and Endangered Plants

Xinyu NI1,2, Junying HE1,2, Mengjiao YAN3, Chao DU1,2()   

  1. 1.College of Life Science and Technology,Inner Mongolia Normal University,Hohhot 010022
    2.Key Laboratory of Biodiversity conservation and Sustainable utilization for College and University of Inner Mongolia Autonomous Region,Hohhot 010022
    3.Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences,Hohhot 010031
  • Received:2022-09-26 Online:2023-07-20 Published:2023-07-03
  • Contact: Chao DU E-mail:duchao@imnu.edu.cn
  • About author:NI Xinyu(1998—),female,postgraduate,majoring in the research of physiological and molecular biology of plant stress.
  • Supported by:
    Natural Science Foundation of Inner Mongolia Autonomous Region(2019LH03005);High-level Scientific Research Foundation for the Introduction of Talent of Inner Mongolia Normal University(2018YJRC014)


Rare and endangered plants are important plant resources in nature, and play a key role in the study of plant phylogeny, ecosystem restoration, plant stress resistance physiology, and the excavation of stress-resistance genes. However, the lack of genetic information has severely restricted the conservation and utilization in most endangered plant. Due to the large genomes, complex genetic information, and unclear genetic backgrounds of endangered plants, it is relatively difficult to sequence the genomes of endangered plants. In recent years, RNA-Sequencing could directly sequence non-reference genomic species, what makes it to be widely used in the study of endangeredplants. In addition, this paper presented the prospect of the application of RNA-Sequencing technology in the study of endangered plants and suggested the new ideas of transcriptome using in the endangered plants in future.

Key words: RNA-Sequencing, endangered plants, stress response, secondary metabolism

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