Isolation and Analysis of Day-night Differential Expressed ESTs from Paraquilegia microphylla

doi:10.7525/j.issn.1673-5102.2011.04.007

Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (4): 414-421.doi: 10.7525/j.issn.1673-5102.2011.04.007

Previous Articles Next Articles

Isolation and Analysis of Day-night Differential Expressed ESTs from Paraquilegia microphylla

LIU De-Tuan;LI Wan-Sha;ZHA Hong-Guang;YANG Yong-Ping;HU Xiang-Yang*

1.Kunming Institute of Botany,Chinese Academy of Sciences,Kunming　650204；2.Graduate University of Chinese Academy of Sciences,Beijing　100049

Received:1900-01-01 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20
Contact: HU Xiang-Yang1
Supported by:

Abstract

Abstract: Suppression subtractive hybridization (SSH) based on magnetic beads technology was used to screen day-night differential expression genes of Paraquilegia microphylla living on the scree of the Baima snow mountain of Tibetan plateau. The daytime sample was used as tester and nighttime sample as driver for further analysis. Finally, 96 differential ESTs between two samples were sequenced. Sequences were analyzed with BlastX, Blast2go and KEGG. The results indicated that most differential expression proteins are related to material and energy metabolism, biosynthetic process, have nucleic acid binding, protein binding and transferase activity; and a few are responsive to stress, etc. These results will provide some prerequisites for further researches on alpine plants under harsh environments.

Key words: alpine plant, stress, suppression subtractive hybridization(SSH)

CLC Number:

LIU De-Tuan;LI Wan-Sha;ZHA Hong-Guang;YANG Yong-Ping;HU Xiang-Yang*. Isolation and Analysis of Day-night Differential Expressed ESTs from Paraquilegia microphylla[J]. Bulletin of Botanical Research, 2011, 31(4): 414-421.

[1]	Luhua ZHOU, Junyi FANG, Zimo XIONG, Weifeng WU, Jiarui LIU, Qiao LU, Hongqing LING, Danyu KONG. Evaluation on Waterlogging Tolerance of Different Tomato Germplasm [J]. Bulletin of Botanical Research, 2023, 43(5): 657-666.
[2]	Dezong SUI, Baosong WANG. Comparative Proteomics on Leaves of Triadica sebifera Clones under Salt Stress [J]. Bulletin of Botanical Research, 2023, 43(5): 679-689.
[3]	Yu SONG, Wenhao LIN, Yibo JING, Yi DONG, Shumei JIN. Cloning and Expression Analysis of Catalase Gene in Lilium pumilum [J]. Bulletin of Botanical Research, 2023, 43(5): 756-767.
[4]	Chaoran SHAN, Xiaohu CHEN, Yunfei DING, Wei ZHAO, Han LU, Shangzhu GAO, Fenghui QI, Yaguang ZHAN, Fansuo ZENG. Functional Analysis of FmCCoAOMT Gene in Fraxinus mandshurica During Lignin Synthesis and Abiotic Stress [J]. Bulletin of Botanical Research, 2023, 43(5): 768-778.
[5]	Xinyu NI, Junying HE, Mengjiao YAN, Chao DU. Application Progress of RNA-Seq Technology in Rare and Endangered Plants [J]. Bulletin of Botanical Research, 2023, 43(4): 481-492.
[6]	Shuyao ZHUANG, Hengbo XU, Xiaoyu HU, Shang DAI, Yanni ZHANG. Effects of Saline-alkali Stress on Growth and Physiological Characteristics of Color Leaf Heuchera micrantha ‘Silver Fan’ Seedlings [J]. Bulletin of Botanical Research, 2023, 43(4): 520-530.
[7]	Lei XU, Xiao XU, Qinsong LIU. Effects of Exogenous Salicylic Acid on Antioxidant System and Gene Expression of Davidia involucrata Seedlings under Salt Stress [J]. Bulletin of Botanical Research, 2023, 43(4): 572-581.
[8]	Yu SUN, Yiteng ZHANG, Huihui CHENG. The Function of Salt and Alkaline Tolerance of WRKY42 Gene in Amorpha fruticosa [J]. Bulletin of Botanical Research, 2023, 43(4): 612-621.
[9]	Hanlin ZHU, Heng ZHAO, Bowen ZHAI, Maoyu ZHANG, Yujie FU. Ultrasonic-Assisted Enzymatic Extraction of Polysaccharides from Schisandra chinensis and the Effects on Anti-Oxidative Stress in Cells [J]. Bulletin of Botanical Research, 2023, 43(4): 631-640.
[10]	Li LI, Xin WANG, Yuejing ZHANG, Lingyun JIA, Hailong PANG, Hanqing FENG. Effects of Abiotic Stresses on the Intracellular and Extracellular ATP Levels of Tobacco Suspension Cells [J]. Bulletin of Botanical Research, 2023, 43(2): 179-185.
[11]	Shixian LIAO, Yuting WANG, Liben DONG, Yongmei GU, Fenglin JIA, Tingbo JIANG, Boru ZHOU. Function Analysis of the Transcription Factor PsnbZIP1 of Populus simonii×P. nigra in Response to Salt Stress [J]. Bulletin of Botanical Research, 2023, 43(2): 288-299.
[12]	Senyao LIU, Fenglin JIA, Qing GUO, Gaofeng FAN, Boru ZHOU, Tingbo JIANG. Response Analysis of Transcription Factor PsnbHLH162 Gene in Populus simonii × P. nigra under Salt Stress and Low Temperature Stress [J]. Bulletin of Botanical Research, 2023, 43(2): 300-310.
[13]	Jianxin LIU, Ruirui LIU, Xiuli LIU, Xiaobin OU, Haiyan JIA, Ting BU, Na LI. Effects of Exogenous Hydrogen Sulfide on Contents of Organic Acids and Hormones in Leaves of Avena nuda under Saline-Alkali Stress [J]. Bulletin of Botanical Research, 2023, 43(1): 76-89.
[14]	Denggao LI, Rui LIN, Qinghui MU, Na ZHOU, Yanru ZHANG, Wei BAI. Identification and Analysis of the Potato StCRKs Gene Family and Expression Patterns in Response to Stress Signals [J]. Bulletin of Botanical Research, 2022, 42(6): 1033-1043.
[15]	Jing WU, Yuanyuan WANG, Danni WANG, Baichao LIU, Zhongyuan LIU. Cloning and Osmotic Stress Response Analysis of ThGRF2 from Tamarix hispida [J]. Bulletin of Botanical Research, 2022, 42(6): 1044-1051.

Isolation and Analysis of Day-night Differential Expressed ESTs from Paraquilegia microphylla

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments