Abstract: The full-length DNA and coding DNA sequence (CDS) of a new 1-aminocyclopropane-1-carboxylate synthase FhACS1 gene was cloned from Fressia hybrida ‘Shangnong Jinhuanghou’ for the first time. DNA sequence of FhACS1 was 2 576 bp long including 433 bp of 5′-UTR and 373 bp of 3′-UTR. Start codon and stop codon located at 434 and 2 201 nt, respectively. This gene contained 3 introns and 4 exons. The full length of CDS is 1 371 bp, encoding a 51.2 kDa protein with 457 amino acid residues. The results showed that FhACS1 contained seven conserved domains of ACS proteins, which was highly homologous to ACS of Musa acuminata with amino acid sequence homology of 85%. Phylogenetic analysis showed that FhACS1 gene in Fressia was closely related to BaACS(BAC56949.1) in bamboo, OsACS1(AAA33888.1) in rice, and MaACS1(AAQ13435) in banana. Several putative cis-elements responding to phytohormones(ABA, GA and CTK) and stress (dehydration and cold) in FhACS1 were predicted using online promoter analysis software.

Key words: Fressia, 1-aminocyclopropane-1-carboxylate(ACC) synthase, gene, sequence analysis, cis-element