Effects of Saline-alkali Stress on Growth and Physiological Characteristics of Color Leaf Heuchera micrantha ‘Silver Fan’ Seedlings

doi:10.7525/j.issn.1673-5102.2023.04.005

Abstract

Abstract:

In order to investigate the effects of saline-alkali stress on the plant growth， seedling of Heucheramicrantha ‘Silver Fan’ was used as materials， and 30， 60， 90， 120 and 150 mmol·L^-1 NaCl and NaHCO₃， and 20， 30， 40， 50 and 60 mmol·L^-1 Na₂CO₃， were used to simulate salt stress， salt alkali stress， and alkali stress， respectively. The experiment used 1/4 Hoagland nutrient solution as the control， and used water culture method to treat seedlings for 0， 7 and 14 d. The response of plants was observed from aspects such as salt damage rate and physiological indicators， and the effects of salt stress on the growth of ‘Silver Fan’ seedlings were analyzed and evaluated by principal component analysis of 7 indicators. The results showed that under different salt alkali stress treatments， the relative conductivity and malondialdehyde content（MDA） of the seedlings showed an increasing trend with time and concentration. The seedlings treated with 30 mmol·L^-1 NaCl or NaHCO₃ showed no obvious symptoms of salt damage， and all physiological indicators showed an upward trend with the prolongation of treatment time； With the increase of concentration and stress time， the salt damage index of seedlings increased， but the ornamental value decreased. The overall trend of superoxide dismutase（SOD） increased first and then decreased， reaching the maximum value at 90 mmol·L^-1 NaCl and 60 mmol·L^-1 NaHCO₃， respectively. Under the same stress time， the contents of chlorophyll（Chl）， chlorophyll a（Chla）， and chlorophyll b（Chlb） decreased continuously， while ρ（Chla）/ρ（Chlb） increased with the increase of stress concentration. Under different concentrations of Na₂CO₃ stress， the SOD activity generally showed an upward trend， reaching the maximum value at 50 mmol·L^-1， and the contents of Chl， Chla， and Chlb generally showed a downward trend. However， under the same time conditions of stress， ρ（Chla）/ρ（Chlb） decreased first and then increased with the concentration increasing， and the ratio of ρ（Chla）/ρ（Chlb） was the smallest under 40 mmol·L^-1 treatment. In general， ‘Silver Fan’ seedlings had a certain salt alkali resistance ability， and could grow at NaCl concentration<90 mmol·L^-1， NaHCO₃ concentration<60 mmol·L^-1， and Na₂CO₃ concentration< 40 mmol·L^-1， and had certain ornamental value， respectively. The results of principal component analysis showed that the relative conductivity， Chla， and Chlb contents could be used as indicators for evaluating the salt alkali resistance of the ‘Silver Fan’.

Key words: Heuchera micrantha, saline-alkali stress, leaves, physiological characteristics

CLC Number:

S682.1+9

Shuyao ZHUANG, Hengbo XU, Xiaoyu HU, Shang DAI, Yanni ZHANG. Effects of Saline-alkali Stress on Growth and Physiological Characteristics of Color Leaf Heuchera micrantha ‘Silver Fan’ Seedlings[J]. Bulletin of Botanical Research, 2023, 43(4): 520-530.

Figures/Tables 12

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References 32

1	刘军，徐金良，邹军，等.盐胁迫对红楠幼苗生长及Na⁺、K⁺吸收和分布的影响［J］.林业科学研究，2013，26（6）：790-794.
	LIU J， XU J L， ZOU J，et al.Effects of salt stress on seedling growth of Machilus thunbergii and Na⁺，K⁺absorption and distribution［J］.Forest Research，2013，26（6）：790-794.
2	姚荣江，杨劲松，刘广明.东北地区盐碱土特征及其农业生物治理［J］.土壤，2006，38（3）：256-262.
	YAO R J， YANG J S， LIU G M.Characteristics and agro-biological management of saline-alkalized land in northeast China［J］.Soils，2006，38（3）：256-262.
3	马书荣，李韫，石美玉，等.盐碱胁迫对蒙古柳无性系生理特性的影响［J］.分子植物育种，2021，19（3）：1004-1013.
	MA S R， LI Y， SHI M Y，et al.Effects of saline-alkali stress on physiological characteristics of Salix linearistioularis clones［J］.Molecular Plant Breeding，2021，19（3）：1004-1013.
4	许盼云，吴玉霞，何天明.植物对盐碱胁迫的适应机理研究进展［J］.中国野生植物资源，2020，39（10）：41-49.
	XU P Y， WU Y X， HE T M.Research progress on adaptation mechanism of plants to saline-alkali stress［J］.Chinese Wild Plant Resources，2020，39（10）：41-49.
5	陈敏，李海云，吕福堂.植物耐盐性研究进展［J］.聊城大学学报（自然科学版），2011，24（3）：47-50.
	CHEN M， LI H Y， LÜ F T.Research progress on plant salt tolerance［J］.Journal of Liaocheng University（Natural Science Edition），2011，24（3）：47-50.
6	景璐，刘涛，白玉娥.草本园林植物耐盐性研究进展［J］.中国农学通报，2011，27（13）：284-289.
	JING L， LIU T， BAI Y E.Advances in adaptability of salt endurance on herbaceous plant［J］.Chinese Agricultural Science Bulletin，2011，27（13）：284-289.
7	李洪瑶，王凯，张永胜，等.盐碱胁迫对两种铁线莲生长状况和生理特性的影响［J］.东北林业大学学报，2018，46（5）：49-54.
	LI H Y， WANG K， ZHANG Y S，et al.Effects of saline-alkali stress on growth and physiological characteristics of two Clematis species［J］.Journal of Northeast Forestry University，2018，46（5）：49-54.
8	李春辉，黄少玲，郭翔，等.我国矾根研究进展［J］.安徽农学通报，2017，23（15）：109-111，134.
	LI C H， HUANG S L， GUO X，et al.Research progress on Heuchera in China［J］.Anhui Agricultural Science Bulletin，2017，23（15）：109-111，134.
9	殷丽青，谢苗苗，孙翊，等.彩叶植物矾根的研究进展［J］.上海农业学报，2018，34（1）：138-143.
	YIN L Q， XIE M M， SUN Y，et al.Research development of colorful plant of Heuchera spp.［J］.Acta Agriculturae Shanghai，2018，34（1）：138-143.
10	SĄKOL G， OCHOTA P， MIROSŁAWSKI J，et al.Suitability of three cultivars of coral bells（Heuchera cvs.） to bioremediation of cadmium（Cd），lead（Pb） and zinc（Zn） from post-mining area in zagłębie dąbrowskie［J］.Medycyna Środowiskowa，2011，14（3）：17-27.
11	SĄKOL G， MIROSŁAWSKI J， BREWCZYŃSKI P Z.Efficient Pb translocation by ‘Purple Petticoats’ cv. of Heuchera L. from contaminated soil in a coal basin［J］.Polish Journal of Environmental Studies，2014，23（6）：2199.
12	曾丽蓉，郑鑫，张婷，等.四种苹果砧木耐盐性差异比较［J］.天津农业科学，2015，21（3）：105-109.
	ZENG L R， ZHENG X， ZHANG T，et al.Salt resistance comparison of four apple rootstocks［J］.Tianjin Agricultural Sciences，2015，21（3）：105-109.
13	王学奎，黄见良.植物生理生化实验原理与技术［M］.3版.北京：高等教育出版社，2015.
	WANG X K， HUANG J X.Principles and techniques of plant physiology and biochemistry experiments［M］.3rd ed.Beijing：Higher Education Press，2015.
14	王佺珍，刘倩，高娅妮，等.植物对盐碱胁迫的响应机制研究进展［J］.生态学报，2017，37（16）：5565-5577.
	WANG Q Z， LIU Q， GAO Y N，et al.Review on the mechanisms of the response to salinity-alkalinity stress in plants［J］.Acta Ecologica Sinica，2017，37（16）：5565-5577.
15	BAHMANI K， NOORI S A S， DARBANDI A I，et al.Molecular mechanisms of plant salinity tolerance：a review［J］.Australian Journal of Crop Science，2015，9（4）：321-336.
16	任鹏飞，尚丽霞，蔡勤安，等.植物耐碱性研究进展及其在大豆中的应用展望［J］.大豆科学，2019，38（6）：977 -985.
	REN P F， SHANG L X， CAI Q A，et al.Research progress of plant alkali tolerance and its application prospect in soybean［J］.Soybean Science，2019，38（6）：977-985.
17	张相锋，杨晓绒，焦子伟.植物耐盐性评价研究进展及评价策略［J］.生物学杂志，2018，35（6）：91-94.
	ZHANG X F， YANG X R， JIAO Z W.Research progress of salt tolerance evaluation in plants and tolerance evaluation strategy［J］.Journal of Biology，2018，35（6）：91-94.
18	VERSLUES P E， AGARWAL M， KATIYAR-AGARWAL S，et al.Methods and concepts in quantifying resistance to drought，salt and freezing，abiotic stresses that affect plant water status［J］.The Plant Journal，2006，45（4）：523-539.
19	MELONI D A， OLIVA M A， MARTINEZ C A，et al.Photosynthesis and activity of superoxide dismutase，peroxidase and glutathione reductase in cotton under salt stress［J］.Environmental and Experimental Botany，2003，49（1）：69-76.
20	魏婧，徐畅，李可欣，等.超氧化物歧化酶的研究进展与植物抗逆性［J］.植物生理学报，2020，56（12）：2571-2584.
	WEI Q， XU C， LI K X，et al.Progress on superoxide dismutase and plant stress resistance［J］.Plant Physiology Journal，2020，56（12）：2571-2584.
21	赵红，徐芬芬，熊安琪，等.不同种类盐胁迫对水稻种子萌发和幼苗生长的影响［J］.分子植物育种，2021，19（17）：5842-5847.
	ZHAO H， XU F F， XIONG A Q，et al.Effects of different salt stress on seed germination and seedling of rice［J］.Molecular Plant Breeding，2021，19（17）：5842-5847.
22	张凤娥，张丽娟，高东菊.混合盐胁迫时2种铁线莲的叶片渗透调节及叶绿素荧光特性［J］.东北林业大学学报，2022，50（5）：52-56，62.
	ZHANG F E， ZHANG L J， GAO D J.Osmotic regulation and chlorophyll fluorescence characteristics in leaves of two Clematis species under saline-alkali stress［J］.Journal of Northeast Forestry University，2022，50（5）：52-56，62.
23	何淼，王欢，徐鹏飞，等.模拟复合盐碱胁迫对芒幼苗生理特性的影响［J］.草业科学，2016，33（7）：1342-1352.
	HE M， WANG H， XU P F，et al.Research on the physiological characteristics of Miscanthus sinensis seedlings under the treatments of complex saline-alkaloid stress［J］.Pratacultural Science，2016，33（7）：1342-1352.
24	陆安桥，张峰举，许兴，等.盐胁迫对湖南稷子苗期生长及生理特性的影响［J］.草业学报，2021，30（5）：84-93.
	LU A Q， ZHANG F J， XU X，et al.Effects of salt stress on growth and physiological characteristics of Echinochloa frumentacea seedlings［J］.Acta Prataculturae Sinica，2021，30（5）：84-93.
25	郑欣颖，李鹏飞，薛立，等.3种园林植物的抗盐生理研究［J］.中南林业科技大学学报，2017，37（9）：62-67.
	ZHENG X Y， LI P F， XUE L，et al.Effect of salt stress on physiological characteristics of garden plants of three species［J］.Journal of Central South University of Forestry & Technology，2017，37（9）：62-67.
26	贾茵，向元芬，王琳璐，等.盐胁迫对小报春生长及生理特性的影响［J］.草业学报，2020，29（10）：119-128.
	JIA Y， XIANG Y F， WANG L L，et al.Effects of salt stress on the growth and physiological characteristics of Primula forbesii ［J］.Acta Prataculturae Sinica，2020，29（10）：119-128.
27	苏世平，刘小娥，席杰.三色堇对NaCl胁迫的生理响应［J］.草业学报，2022，31（8）：90-98.
	SU S P， LIU X E， XI J.Physiological response of Viola tricolor to NaCl stress［J］.Acta Prataculturae Sinica，2022，31（8）：90-98.
28	潘瑞炽.植物生理学［M］.北京：高等教育出版社，2001：57-62.
	PAN R C.Plant physiology［M］.Beijing：Higher Education Press，2001：57-62.
29	梁新华，谢亚军，田峻颉.NaCl与Na₂CO₃胁迫对光果甘草幼苗叶片叶绿体色素含量的影响［J］.中国农学通报，2005，21（9）：228-230.
	LIANG X H， XIE Y J， TIAN J J.Effect of NaCl and Na₂CO₃ stress on the content of chloroplast pigment in leaves of Glycyrrhiza glabra seedlings［J］.Chinese Agricultural Science Bulletin，2005，21（9）：228-230.
30	张丽，徐志然，胡晓辉，等.叶面喷施亚精胺对盐碱胁迫下番茄幼苗生长及其叶绿素合成前体含量的影响［J］.西北植物学报，2015，35（1）：125-130.
	ZHANG L， XU Z R， HU X H，et al.Effect of foliar-spraying spermidine on seedlings growth and contents of chlorophyll biosynthesis precursors in leaves of tomato under saline-alkaline stress［J］.Acta Botanica Boreali-Occidentalia Sinica，2015，35（1）：125-130.
31	张小艾，李名扬，汪志辉，等.重金属及盐碱对二月兰幼苗生长和生理生化的影响［J］.草业学报，2013，22（2）：187-194.
	ZHANG X A， LI M Y， WANG Z H，et al.Effects of heavy metals and saline-alkali on seedlings growth，physiological-biochemical of Oryehophragmus violaeeus ［J］.Acta Prataculturae Sinica，2013，22（2）：187- 194.
32	李雅男，袁素霞，徐雷锋，等.百合在NaHCO₃胁迫下的生理响应机制［J］.园艺学报，2016，43（6）：1126-1140.
	LI Y N， YUAN S X， XU L F，et al.The physiological response of lily to NaHCO₃ stress［J］.Acta Horticulturae Sinica，2016，43（6）：1126-1140.

胁迫处理 Stress treatment	处理浓度 Treatment concentration	盐害指数 Salt damage index/％	盐害率 Salt damage rate/％	胁迫处理 Stress treatment	处理浓度 Treatment concentration	盐害指数 Salt damage index/％	盐害率 Salt damage rate/％	胁迫处理 Stress treatment	处理浓度 Treatment concentration	盐害指数 Salt damage index/％	盐害率 Salt damage rate/％
NaCl	30	0	0	NaHCO₃	30	0	0	Na₂CO₃	20	0	0
	60	33.33	33.33		60	33.33	33.33		30	33.33	33.33
	90	66.67	66.67		90	66.67	100.00		40	33.33	66.67
	120	66.67	100.00		120	77.78	100.00		50	66.67	66.67
	150	88.89	100.00		150	83.33	100.00		60	77.78	100.00

主成分 Principal component	负载权数Load weights							特征值 Eigenvalue	贡献率 Contribution rate/％	累积贡献率 Cumulative contribution rate/％
主成分 Principal component	MDA含量 MDA content	相对电导率 Relative conductivity	SOD活性 SOD activity	叶绿素a含量 Chla content	叶绿素b含量 Chlb content	ρ（Chla）/ρ（Chlb）	叶绿素含量 Chl content	特征值 Eigenvalue	贡献率 Contribution rate/％	累积贡献率 Cumulative contribution rate/％
1	-0.773	-0.853	0.291	0.985	0.970	-0.007	0.075	3.327	47.524	47.524
2	0.630	0.391	0.517	0.099	0.151	0.979	0.086	1.816	25.947	73.471
3	0.065	0.323	-0.756	-0.098	0.171	-0.080	0.976	1.679	23.984	97.455

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[10]	LI Dong-Lin, JIN Ya-Qin, CUI Meng-Fan, HUANG Lin-Xi, PEI Wen-Hui. Effects of Shading on Physiological Characteristics and Ultrastructure of Mesophyll Cell of Emmenopterys henryi Leaves [J]. Bulletin of Botanical Research, 2020, 40(1): 29-40.
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