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    15 September 2002, Volume 22 Issue 3
    A NEW SPECIES OF SACCIOLEPIS FROM YUNNAN PROVINCE
    QIAN Yi-yong
    2002, 22(3):  257-258. 
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    A new species of Sacciolepis Nash from Yunnan province was reported.
    A NEW SPECIES OF PERIPLOCA FROM GANSU PROVINCE
    YAO De-sheng, CHEN Xi-cang, REN Ji-wen
    2002, 22(3):  259-260. 
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    Periploca chrysantha D. S. Yao, X. C. Chen et J. W. Ren. is given.
    A NEW VARIETY OF VIBURNUM FROM SHANDONG PROVINCE
    ZHU Ying-qun, XIE Lan-yu, XU Ji-fa, WU Bao-li, LIANG Zi-shan, ZHANG Yu-fang
    2002, 22(3):  261-261. 
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    A new variety species of Viburnum is given.
    EIGHT MARINE SPECIES OF THE CLADOPHORACEAE(CHLOROPHYTA) NEW TO CHINA
    LUAN Ri-xiao, LUAN Shu-jun
    2002, 22(3):  262-270. 
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    Eight species of the Cladophoraceae, Cladophora arenaria Sakai, C. conchopheria Sakai, C. opaca Sakai, C. pellucida(Hudson) Kuetzing, C. pusilla Sakai, C. rudolphiana(C. Agardh) Harvey, C. sericea Sakai C. speciosa Sakai are reported from the coast of Liaoning, Shandong and Jiangsu, China. All the eight species are new to China.
    NEW DATA FOR MAGNOLIACEAE PLANTS IN YUNNAN PROVINCE OF CHINA, THAILAND AND VIETNAM
    SIMA Yong-kang, Piya Chalermglin
    2002, 22(3):  271-272. 
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    The paper presents some new data of Magnolia and Manglietia in China(Yunnan), Thailand and Vietnam. The voucher collections are kept in China(YCP).
    EXAMINATION ON THE TYPE OF ZELKOVA SPACH(ULMACEAE)
    SA Ren, Sudebilige
    2002, 22(3):  273-274. 
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    Based on International Code of Botanical Nomenclature and examination of related materials, it is certain that the type of Zelkova Spach is Z. carpinifolia(Pall.) K. Koch., but not Z. crenata Spach or Z. carpinifolia(Pall.) Dipp. as usually recognized.
    ON THE PROBLEM OF DEMARCATION BETWEEN ASTRAGALUS LINN. AND CHESNEYA LINDL. EX ENDL.——WITH REFERENCE TO MY KNOWLEDGE OF ASTRAGALUS MILINGENSIS NI ET P. C. LI
    ZHU Xiang-yun
    2002, 22(3):  275-277. 
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    This paper deals with the demarcation between Astragalus and Chesneya. For example, the species Astragalus milingensis was published by Ni & Li in 1979, but it was treated as a species of Chesneya namely Chesneya milingensis by Zhu & Cao in 1986. Based on my researches, this species should be treated as a species of Astragalus. The two genera Astragalus and Chesneya have some clear characteristics as their essence.
    A PRELIMINARY DISCUSSION ON THE TAXONOMIC VALUES OF THE MAIN CHARACTERS OF REPRODUCTIVE ORGAN OF POACEAE AND THEIR RANKS SUITABLE FOR DIFFERENTIATING TAXA
    CAI Lian-bing
    2002, 22(3):  278-284. 
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    The characters of reproductive organ have been the main evidences for the classification of Poaceae, but they are not totally equal in the taxonomic value because of the differences of their dependencies in morphological trait, growth position, developmental degree and environmental alteration. In order to overcome the various drawbacks in applying character, author discussed the taxonomic values of main macroscopic characters in the paper, and summarized their ranks suitable for differentiating taxa. In them, the type of inflorescence, position of spikelet, shape of floscule and fruit, and so on are of high value to classification, and usually suitable to the taxa above the rank of tribe;the shape of inflorescence, arrangement of spikelet, type of fruit, and so forth are slightly low in value, and usually suitable to the taxa from genus to species;the length and thickness of inflorescence, rachilla, palea, and the like are low in value, and usually suitable to the species or infraspecific taxa.
    THE TYPE AND EVOLUTION OF VASSEL ELEMENT'S PENFORATION PLATE OF SORBUS POHUASHANENSIS
    ZHU Jun-yi
    2002, 22(3):  285-287. 
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    Educing and observing the stem's secondary xylem of Sorbus pohuashanensis revealed two types of perforation plates, simple porforation plate and reticulate perforation plate. There are 5 transition types. There are 3 types of vassel elements:both ends are reticulate type;one end is reticulate type, the other simple type;both ends are simple type. The transform of perforation plate types in ontogenesis process of Sorbus pohuashanensis recapitulates the evolution process of perforation plate from reticulate type to simple type in phylogeny.
    INDUCTION, DIFFERENTIATION AND PLANT REGENERATION OF TRIPLOID CALLI OF POPULUS TOMENTOSA
    LI Yi, HE Ming-zhu, MA Hai-yun
    2002, 22(3):  288-291. 
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    Regenerating plants were produced from immature leaves of the triploid of Populus tomentosa by tissue culture on MS basic medium with different plant hormone. The result showed that callus induction frequency reached to 87.6 percent on MS+6-BA 0.5mg/L+NAA 0.1mg/L;differentiation frequency reached to 68.5 percent on 12MS+IAA 0.1mg/L+6-BA 0.1. It can be generated from one to 7.4 axillary shoots on MS+6-BA 0.5mg/L+NAA 0.01mg/L. Rooting frequency of the shoots got to 85.7 percent onMS+IBA 1.0mg/L.
    EFFECTS OF NaCl STRESS ON CELL DIVISION OF WHEAT WITH DIFFERENT DROUGHT-TOLERANCE
    ZHANG Hai-yan
    2002, 22(3):  292-295. 
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    Results indicated that mitotic index of radicular cells of wheat with different drought-tolerance(Lumai-14 and Jinhan-47)decreased under NaCl stress. Aberration rates of nucleus and chromosome were directly proportional to NaCl concentration and treated time. Results also showed that salt tolerant capability of Jinhan-47 was much stronger than that of Lumai-14.
    STUDY ON THE RELATIONSHIP BETWEEN THE CHANGES OF ACTIVITIES AND ISOENZYMES OF PEROXIDASE AND THE ROOTINGOF EUCALYPTUS CUTTINGS AFTER TREATMENT WITH NAPHTHYLACETIC ACID
    HUANG Zhuo-lie, LI Ming, TAN Shao-man, LIN Shao-xiang, LONG Teng
    2002, 22(3):  296-300. 
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    Eucalyptus urophylla S. T. Blade MLA clone(MLA) was difficult-to-root species. E. urophylla S. T. Blade U6 clone(U6) and E. ABL 12 W5 clone(W5) were relatively easy-to-root species. The activities of peroxidase(POD) in MLA were higher than that in U6 and W5. After the cuttings of Eucalyptus were treated with naphthylacetic acid(NAA), the activities of POD increased regularly in different stages of rooting. The relationship between peroxidase and rooting of Eucalyptus cuttings was discussed.
    NUTRITIVE MATERIAL IN CUTTINGS OF LARIX OLGENSIS AND ITS EFFECT ON ROOTING
    AO Hong, WANG Kun, CHEN Yi-ling, ZHANG Jie
    2002, 22(3):  301-304. 
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    Besides endogenous plant hormones, nutritive material is another important factor that affects the rooting of Larix olgensis. The results of experiment demonstrated that dissolvable sugar and nitrogen levels exist divergence in cuttings of different age of seed-tree, They were higher in cuttings of young seed-tree. It is proved by experiments that nutrition especially the dissolvable sugar and nitrogen levels are important. When the contents are plentiful, the rate of rooting are higher, and the ratio between dissolvable sugar and nitrogen is not a ideal target to express the capability of rooting of Larix olgensis.
    THE EFFECTS OF SULFUR DIOXIDE DERIVATIVES ON THE GROWTH AND CELL CYCLE OF VICIA FABA SEEDLINGS
    YI Hui-lan, SI Liang-yan, MENG Zi-qiang
    2002, 22(3):  305-309. 
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    The effects of the derivatives of sulfur dioxide(sodium bisulfite) on the cell division and growth of V. faba seedlings were investigated. The results showed that bisulfite inhibited seedling growth and both length of buds and roots had negative relation to the concentrations(in the range of 0~30mmol/L). The mitotic index decreased significantly in root tips when seedlings exposed to sodium bisulfite for 12~36 h. The number of prophase cells was decreased but the number of the cells at interphase and anaphase and telophase increased after exposure 12~36 h. The results indicate sodium bisulfite prevent the cells from going split and delay the mitosis process, the effects may include delays in passing through S into G2, a protracted G2 and an effect on the speed of passage through mitosis itself. That may be the cause for the growth inhibition and the decrease in mitotic index.
    DNA MICROARRAY AND ANALYSIS OF DIFFERENTIAL GENE EXPRESSION IN PLANT
    LI Tong-xiang, WANG Jin-ke
    2002, 22(3):  310-313. 
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    DNA microarray technology has made a revolutionary transformation in studying differential gene expression, the collaboration of multigene for one trait, discovering and locating target genes, among different individual in a same or different species and one plant individual in different development stage as well as between normal and diseased condition. The major advance of DNA microarray technology, as compared to conventional techniques, results from the small size of the array, which allows for a higher accurate, a quicker process and a higher sensitivity enables the parallel screening of larger number of genes simultaneously on one chip and provides the opportunity to use smaller amounts of starting material. Many significant achievements have been obtained in picturing the expression profiles of genes, discoverying the target genes and elucidating gene functions with DNA microarray on plant, such as Arabidopsis and yeast, rice.
    RAPD ANALYSIS OF CYCLAMEN PERSICUM GERMPLASM
    CHE Dai-di, QIN Zhi-wei, WANG Jin-gang
    2002, 22(3):  314-317. 
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    RAPD analysis was applied to Cyclamen(Cyclamen persicum Mill.) in order to assess the classification and genetic relationships among 20 cultivars of Cyclamen 18 stochastic selected from 100 ones were used to PCR reaction bands were amplified in 153 dots in all. Each primer amplified 8.5 dots, 144 poly in orphism dots equally. These dots were 94.1% of all. This polymorphism could identify cultivars. Every cultivar resource was set apart into 4 groups by the analysis of grouped together. Cyclamen should has more scientific classified method based on gene besides ornamental character at present. The result also revealed Cyclamen cultivar's stricture of genetic base in the level of molecule. The farther application of RAPD in Cyclamen had been discussed.
    RESEARCH ON CONSTRUCTION OF AN EXPRESSION VECTOR OF CHITINASE GENE AND ITS TRANSFORMATION INTO TOBACCO
    XU Xiang-ling, LI Ji-lin, MA Xing-hong
    2002, 22(3):  318-321. 
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    Expression vector containing a chitinase gene and some regulatory sequences was in this reseach. Using the Ri plasmid of Agobacterium. rhizogenes, the chitinase gene was introduced into tobacco, three cultivars of tobacco. Seven plants were proved of be transformed tobacco by PCR detection, by dot blot hybridization. The result sussgested that the construction of the expression vector is successful and the chitinase gene had integrated into tobacco genome.
    RAPD DIVERSITY OF GERMPLASM RESOURCES ON PHASEOLUS VULGARIS
    LUAN Fei-shi, ZU Yuan-gang
    2002, 22(3):  322-327. 
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    Sixty varieties of Phaseolus vulgaris were collected in this study, including 43 from Heilongjiang Province, 13 from The Center of International Agriculture Tropic and 4 from Poland, which were classified as 35 for vine, 12 for dwarf and 13 for semi-wild variety. This study was to analyze the genetic similarity and genetic distance in population and among populations of the 60 varieties and to draw the dendrogram of cluster analysis by using the markers of RAPD. Total 60 varieties in this study could be classified into 5 groups for vine population, 2 for dwarf population and 2 for semi-wild population based on the analysis of the dendrogram of cluster analysis for the markers of RAPD. It is of importance to collect Phaseolus vulgaris resources from home and abroad since Heilongjiang Province is the main area for breeding and cultivating Phaseolus vulgaris in China. The analysis for the markers of RAPD would be beneficial for investigating the genetic background of Phaseolus vulgaris in population and among populations and for identifying the inter-relationship in population. The present study would be valuable for preserving the germplasm and breeding good strains of Phaseolus vulgaris in Heilongjiang Province.
    EFFECT OF SALT STRESS ON SEED GERMINATION OF LOLIUM PERENNE L. AND FESTUCA ELATA KENG.
    LU Jing-jun, LI Qiang, DUO Li-an
    2002, 22(3):  328-332. 
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    Two kinds of turf grasses, Lolium perenne L. and Festuca elata Keng., were treated with different concentration of NaCl, KCl, MgSO4 and the mixture of the three salts above for 15 days. Their relative germination percentage, growth status of their roots and leaves and ecological limit analysis were determined. The results showed that stronger adaptation of Lolium perenne L. and Festuca elata Keng. to the saline environment were approved. Lower concentrations of NaCl, KCl, MgSO4 and their mixture promote seed germination of Festuca elata Keng.. However germination percentage decreased prominently with increasing of NaCl, KCl and the mixture concentration, different salts affected the seed germination percentage unsignificantiy. Higher concentration of the salts delayed the seed primary germination time and inhibited the seedling normal growth. MgSO4 had no prominent inhabitation to the germination percentage of Lolium perenne L. and Festuca elata Keng., but roots growth of Lolium perenne L. was inhibited significantly.
    RESEARCH OF TUBE STORAGE TECHNIQUE AND ITS EFFECTS OF TABACCO CONTAINED VIRUSES
    GUO Zhi-ping
    2002, 22(3):  333-336. 
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    Through three-yesr's experiment of storing tabacco contained PVX. PVY. TMV viruses, a conclusion is arrived at that the tabacco can grow normally iy tube. It is suggested by counter-dry-transfer process and immunological electron microscope capture that the viruses content vary at the tabacco's various grouth stage in tube, and that tabacco can grow normally outside the tube, showing relevant symptoms. The experiment of inoculation indicates that its infectivity is equal to that of viruses stored inside.
    A COMPARATIVE STUDY ON CONTENTS OF SALIDROSIDE IN RHODIOLA SACHALINENSIS ROOT MEASURED BY HIGH PERFORMANCE CAPILLAY ELECTROPHORESIS
    GU Hui-yan, GONG Li-dong, MA Ling
    2002, 22(3):  337-340. 
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    The contents of salidroside in two populations of varied wild growing colonies and two samples of cultivated root of Rhodiola sachalinensis at different places were measured by high performance capillary electrophoresis comparatively. The results demonstrated that analytical method is of high performance, simple, sensitive, and reliable. The contents of salidroside in cultivated and wild growing roots of Rhodiola sachalinensis present a difference, and the contents in different natural colonies show varied too.
    ON THE SYSTEMATICS, EVOLUTION, FLORISTICS AND ECONOMIC USES OF PALMAE AND ITS SISTER FAMILY, CALAMACEAE
    LING Yeou-ruenn
    2002, 22(3):  341-365. 
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    Author provides, in the paper, that the Calamaceae is a transitionary Family, from Subfamily, Calamoideae of Palmae, by covering the reflexing spirally scales, which are diferrent from Palmae, on ovaries and fruits. The Ancestor of Palmae, Pro-Palmae, perhaps, was derived, in Cretaceous Period, from a branch of the evolution trunk from Caytoniales to Pro-Cycadales in Carboniferous Period according to the materials of fossial and pollen grains of Paleobotany, and the ancestor of Calamaceae, Pro-Calamaceae, perhaps, was derived from the branchlet on the evolution branch to the Palmae in later Cretaceous Period. Both Palmae and Calamaceae, especially the former, maybe, is one of the most primitive families of Angiospermae, especially inMonocotyledoneae. In Palmae the Phytelephantoideae and Coryphoideae should be the most or more primitive groups, and Arecoideae and Ceroxyloideae are more advanced groups. But Nypoideae, perhaps, is the most advanced and specialized one, even the ancestor of it, perhaps, was derived from Pro-Phytelephantoideae, In Calamaceae, the Calamoideae is more primitive one than the Lepidocaryoideae. Author also discusses the characters of floristics of two families. Although they are pan-tropic distribution, only a few genera of them are extended to warm and central subtropical areas in both North and South Hemispheres. The margin of distribution are extended to the Yangtze River Valley and the South of the lower reaches of Yellow River of China, Central of Japan, California and Florida of USA and the North of Mediterrenian areas in North Hemisphere, and to Central of Chile and South of New Zeyland in South Hemisphere. The present distributional center are in tropical and warm subtropical areas of Asia, Central and South America, Oceania and Africa. The mass center of it is in Tropical Asia, Tropical Central and South America, South Pacific Archipelagoes and S.-E. Africa. Finally, author introduces in the past 50 years more than 400 species of two families have been introduced and acclimatized in S., S.-E. and S.-W. China, except the native species in China. Some cold-resistant species were introduced into the Yangtze River Valley or even more northern areas. Most species, in above mentioned, are in the important economic uses, such as 1. The eadables, included the starch from pith and juice from trunk or inflorescences for food, or making sugar, wine, vinegar or drink, or isolated oil from fruits or seeds for eating or for industral uses. Some berries and drupes are used for eating fruits, and some tender buds are for vegetables. Also a few seeds are the substitutors of Coffe. 2. Medicinal uses, which included many species are for the antiphlogistic, the anthelmintic, the haemostatic or invigorating the circulation of blood and for anti-cancers;3. Constructions, included that some timbers are for building the common houses, bridges, boats;abstracting wax is for industral or civil uses;fibres-for weaving thick cables or ropes and rugs, and others are for making the handicrafts, and articles of everyday uses;4. Gardening, those are used for building the tropical scenic spots, beautifing the gardens and residential quarters or constructing the landscape ecology etc.
    THE STUDY ON PINUS BUNGEANA COMMUNITY CHARACTERISTICS AND SPECIES DIVERSITY IN THE SOUTH OF MT. LULIANG
    BI Run-cheng, CHENG Ya-li, YIN Da-ze, WEI Xue-zhi, LI Xiao-qiang, HUANG Lei, CUI Ben-yi, WEI Wen-long
    2002, 22(3):  366-372. 
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    The distributing centers of Pinus bungeana community on Mts. Liujia and Mts. Heihugou were selected as 2 stands and were designed 26 sampling lands. 426 plots were investigated by sampling method. Based on the plot data, Pinus bungeana community characteristics were analysed about the specific composition;the flora feature;the community physiognomy;the vertical and level structure;and the regulation of environment. The indexes of the frequency and the coverage and the species diversity(Shannon-Wiener index; Simpson index;Pielou index and Probility of Interspecies Encounter index) were measured in Pinus bungeana community in the sampling stands. The results are as following. The community of Pinus bungeana apparently distributed at 900~1400m in Lcies, which the genus of the boreal temperate zone is absolute dominance。The species and quantity of hemicryptophyte were more and larger. In Mt. Liujia, there was a striking dominant synusia of P. bungeana in the P. bungeana community, which was a monodominant community. The H index of tree layer was 0.706, brush layer 2.351, herb layer 1.257. But in Mt. Heihugou, tree layer was some monodominant community, the H index was 1.825;brush layer 2.308;herb layer 2.400. This discussion aims at scientific sustainable development and conservation of this rare phytobiocenose. uliang ranges;the specific composition of the community is about 70 spe.
    THE CHARACTERISTICS OF THE SEED PLANT FLORA IN LOESS PLATEAU
    ZHANG Wen-hui, LI Deng-wu, LIU Guo-bing, XU Xue-hua
    2002, 22(3):  373-379. 
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    The flora of loess plateau belongs to a Subregion in the North China Region in the Japan-China Forest Plant Subkingdom in the Holarctic Kingdom in Chinese flora, which is the crisscrossing and assembling place of xerophyte, mesophyte and hygrophyte from south to north and from east to west. There are 3224 species(including infraspecific taxa) of the seed plants(belonging to 864 genera, 147 families) in loess plateau. Among them, 41 species(belonging to 13 genera and 7 families) are of gymnosperm and 3183 species(belonging to 851 genera, 140 families) of angiosperm. In addition, 32 genera in loess plateau are endemic to China, and 164 species are endemic to loess plateau. Based on the analysis of the flora, the paper has discussed the major characteristics of the flora. It is comparatively rich in species, ancient in origin, complex, varied in geographical elements, and dominant in the temperate. The seed plant flora of loess plateau has widely related to other neighbor flora. Therefore, the flora is characteristics by the crisscrossing and assembling of diversified geographical elements. Compared with other area, the seed plant flora is less dominant in endemism, but more abundant in rare and endangered species.
    STUDY ON THE PTERIDOPHYTE OF WULINGYUAN FROM HUNAN PROVICE, CHINA
    WU Shi-fu, LI Xin-guo, ZHAO Hui-fang
    2002, 22(3):  380-384. 
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    A preliminary study of the pteridophyte flora of Wulingyuan in Hunan Province, of China is carried out. The floristic compositions, ecological types, distribution and the affinities with its neighbor regions are discussed. In this region, 256 species including varieties and forms of pteridophytes belonging to 86 genera, which are divided into 12 distribution types, in 38 families are recorded. The pteridophyte flora of Wulingyuan is belonging to east and central China region and closely related to southwestern China region. Three vertical distributions of pteridophytes, i. e. lower mountane, moutane and summit mountane, are divided. Further investigation in northwestern region of Hunan province is necessary.