Cloning and Expression Analysis of DELLA gene HbRGL1 from Hevea brasiliensis Müll. Arg

doi:10.7525/j.issn.1673-5102.2022.06.009

Abstract

Abstract:

DELLA protein belongs to the plant-specific GRAS family and is a negative regulator of plant growth. To reveal the molecular regulation mechanism of DELLA gene in rubber tree growth and development， the full-length cDNA sequence of HbRGL1 was cloned from rubber tree cultivar Reyan 7-33-97. The ORF of HbRGL1 was 1 851 bp， and encoded 616 amino acids. Bioinformatics analysis showed that HbRGL1 was an unstable hydrophilic protein containing two conserved domains of DELLA and GRAS， and localized in the nucleus. Phylogenetic analysis showed that HbRGL1 was clustered with HbGAIL of rubber tree， MeGAIL of Manihot esculenta， RcGAI of Ricinus communis， and JcGAIof Jatropha curcas. qRT-PCR analysis showed that the expression level of HbRGL1 in different tissues of rubber treewas significantly different， and its expression level was the highest in flower. HbRGL1 expression was significantly up-regulated in leaves treated by different hormones such as auxin（IAA）， ethephon（ET） and abscisic acid（ABA）. Especially， and it rapidly responded to GA₃ treatments， and had the highest up-regulation of expression level. The study provided theoretical basis for further elucidating the function of HbRGL1 in the growth and development of rubber tree.

Key words: Hevea brasiliensis, HbRGL1, gibberellin, gene expression

CLC Number:

S794.1

Bi QIN, Mingyang LIU, Meng WANG, Lifeng WANG, Fei HUANG. Cloning and Expression Analysis of DELLA gene HbRGL1 from Hevea brasiliensis Müll. Arg[J]. Bulletin of Botanical Research, 2022, 42(6): 997-1004.

Figures/Tables 6

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引物名称 Primer name	引物序列 Primer sequence （5′—3′）
HbRGL1-F	GAAAGATGAAAAGAGATCACCAAGAAA
HbRGL1-R	ACTGACTCGCTAACATTAACTCACC
HbRGL1-QF	CAAGCGAGTATGTTACTAGCAC
HbRGL1-QR	CTATTCCGAACCACCGAGTTT
HbACTIN-F	GATGTGGATATCAGGAAGGA
HbACTIN-R	CATACTGCTTGGAGCAAGA

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