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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (1): 39-46.doi: 10.7525/j.issn.1673-5102.2022.01.005

• Research Report • Previous Articles     Next Articles

Cloning and Disease Resistance Response Potential of Walnut JrNPR1 Gene

Kaiheng Ma1,2, Jianing He1,2, Muhong Xie1, Bin Ren1,2, Qianxue Huang1, Guiyan Yang1,2()   

  1. 1.Shaanxi Walnut Engineering Technology Research Center,College of Forestry,Northwest A&F University,Yangling 712100
    2.Key Laboratory of Economic Plant Resources Development and Utilization in Shaanxi Province,College of Forestry,Northwest A&F University,Yangling 712100
  • Received:2020-11-13 Online:2022-01-20 Published:2021-12-30
  • Contact: Guiyan Yang E-mail:yangguiyan@nwsuaf.edu.cn
  • About author:Ma Kaiheng(1983—),female,master,technician,mainly engaged in forest genetic breeding research and laboratory management.
  • Supported by:
    National Natural Science Foundation of China(31800510)


Nonexpressor of pathogenesis-related gene 1(NPR1) played a key role in regulating plant disease resistance. Walnut(Juglans regia) was an important economic oil tree for rural revitalization in China, and its growth and yield were severely restricted by pests and diseases. In order to explore the physiological mechanism of walnut disease resistance and screen disease resistance genes, the JrNPR1 gene was cloned from ‘Xiangling’ walnut, and its basic biological information and disease response were analyzed to predict the function of JrNPR1. The results showed that the ORF of JrNPR1 gene was 1 782 bp, encoded 593 amino acids, and the theoretical isoelectric point was 6.40. Multi-sequence comparison with Morella rubraRicinus communis and other homologous proteins showed that they all had NPR1-like-C conserved domains, and JrNPR1 had a relatively close evolution with M. rubra and Quercus suber. The upstream 1 233 bp promoter contained a variety of cis-acting elements related to plant disease resistance, such as WRKY71OS. Under treatment with pathogens such as Colletotrichum gloeosporioidesMelanconium oblongum, and Xanthomonas campestrisJrNPR1 was significantly induced, and its expression was up-regulated by salicylic acid(SA). The expression level of JrNPR1 under CgTJ+SA, MoZK+SA, XcHB+SA was 1.31~178.89 fold of that under single stress of CgTJ, MoZK, XcHB, respectively. These results suggested that JrNPR1 might be an important disease resistance gene against walnut anthracnose, branch blight and bacterial black spot, and might be involved in the SA signal pathway.

Key words: Juglans regia, NPR1 gene, gene expression, disease resistance

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