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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (2): 302-311.doi: 10.7525/j.issn.1673-5102.2021.02.018

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Effect of Exogenous ATP on Cold Tolerance of Brassica campestris Seedlings

Xin-Dan HU, Yao LI, Xiao-Hua ZHANG, Juan-Hong LIANG, Teng-Guo ZHANG()   

  1. College of Life Sciences,Northwest Normal University,Lanzhou 730070
  • Received:2019-12-05 Online:2021-03-20 Published:2021-01-05
  • Contact: Teng-Guo ZHANG E-mail:zhangtengguo@163.com
  • About author:HU Xin-Dan(1993—),female,master student,mainly engaged in the study of physiological and molecular biology.
  • Supported by:
    the National Natural Science Foundation of China(31460099)


The effects of exogenous ATP on the cold tolerance of rape seedlings were studied with “Longyou 7”Brassica campestris L. as experimental material. Compared with low temperature stress alone, MDA content and content of rapeseed seedlings were significantly decreased after exogenous ATP pretreatment and low temperature stress, while chlorophyll content and antioxidant enzyme activities(SOD, POD, CAT, APX) were observed. The expressions of RBOHDRBOHFCPK4 and CPK5 were increased. Compared with exogenous ATP+ low temperature, MDA content was significantly increased under EGTA+exogenous ATP+ low temperature treatment. Total chlorophyll content, T-AOC enzyme activity, RBOHD and RBOHF gene expression were all increased. Significantly decreased, MDA content was increased significantly under DMTU+exogenous ATP+low temperature treatment, total chlorophyll content, Ca2+-ATPase activity, CPK4CPK5 gene expression were decreased, indicating that exogenous ATP affected cold tolerance of rape seedlings by Ca2+ and H2O2-dependent mechanisms.

Key words: Brassica campestris L., exogenous ATP, low temperature stress, calcium, antioxidant enzymes

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