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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (6): 953-960.doi: 10.7525/j.issn.1673-5102.2017.06.020

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Cloning and Expressional Analysis of HbMYB62 under Multi-stimulation in Hevea brasiliensis Muell. Arg.

LU Yan-Xi1, ZHANG Dong1, WANG Li-Feng2, WANG Ji-Kun2   

  1. 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228;
    2. Danzhou Investigation and Experiment Station of Tropical Crops, Ministry of Agriculture, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737
  • Received:2017-05-03 Online:2017-11-15 Published:2017-11-25
  • Supported by:
    National Natural Science Foundation of China(31570591,31270643)

Abstract: R2R3-type MYB transcription factor participates in versatile biological process including stress response process. To identify the structure and function of the MYB gene in rubber tree, the full length cDNA of HbMYB62 was cloned in leaves of rubber tree. It was 1 013 bp in length, containing a 945 bp ORF which encodes 314 amino acid residues. The deduced amino acid sequence had the specific domains of plant HTH_MYB DNA binding domain. It showed the highest identity with AtMYB62 in all AtMYB family. This gene is mainly expressed in flower, whilst less expressed in bark, latex and leaf. The gene expression of HbMYB62 were differentially upregulated by H2O2, ABA, and SA. The HbMYB62 gene may play an important role in stress resistance and plant hormones signal transduction pathway in rubber tree. This study provides a fundamental knowledge for further study the structure and functions of HbMYB62.

Key words: Hevea brasiliensis, HbMYB62, wounding, hormone, gene expression

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