Regulation Mechanism of Endogenous Hormones in Adventitious Roots Formation of Platycladus orientalis ‘Beverleyensis’

doi:10.7525/j.issn.1673-5102.2022.02.013

Abstract

Abstract:

Platycladus orientalis ‘Beverleyensis’ is an important ornamental tree species. Plant endogenous hormones（i.e.， indole-3-acetic acid（IAA）， zeatin（ZT）， abscisic acid（ABA） and jasmonic（JA）） play an important role in regulating the adventitious roots regeneration process， but the dynamic changes of endogenous hormones at different developmental stages and their effects on adventitious roots are still unclear. Using semi-woody branches as materials， the dynamic changes of four endogenous hormones were detected by high performance liquid chromatography- mass spectrum（LC-MS）. The results showed that the root primordium originated from callus， medullary ray， xylem， vascular cambium， secondary phloem， cortex， and the junction of medullary ray and cambium respectively， which belonged to multiple locus occurrence mode and the multiple rooting type. Hormonal dynamics results showed that the content of IAA and ZT decreased， ABA and JA increased with the formation of callus； the content of IAA and ZT increased slowly， ABA and JA decreased with the differentiation of root primordium； IAA， ZT， JA gradually increased， and ABA maintained at a low level during adventitious root formation and elongation. Hormone balance analysis results showed that the IAA/ABA ratio and IAA/JA ratio both decreased， and the increasing of IAA/ZT ratio was beneficial to the formation of callus， and on the contrary， it was beneficial to the induction and differentiation of root primordia， while the IAA/ABA ratio increased， IAA/ZT and IAA/ JA maintained at a low level was conducive to the formation and elongation of adventitious roots. The results provide a basis for revealing the regulation effect of different endogenous hormones on the development of adventitious roots of P. orientalis ‘Beverleyensis’.

Key words: Platycladus orientalis ‘Beverleyensis’, adventitious roots, endogenous hormones, root primordium, dynamic balance

CLC Number:

Q944.54

Guobin Liu, Ting Liao, Ye Wang, Liqin Guo, Jinzhe Zhao, Yanwu Yao, Jun Cao. Regulation Mechanism of Endogenous Hormones in Adventitious Roots Formation of Platycladus orientalis ‘Beverleyensis’[J]. Bulletin of Botanical Research, 2022, 42(2): 278-288.

Figures/Tables 5

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References 44

1	黎家，李传友.新中国成立70年来植物激素研究进展［J］.中国科学：生命科学，2019，49（10）：1227-1281.
	Li J，Li C Y.Seventy-year major research progress in plant hormones by Chinese scholars［J］.Scientia Sinica Vitae，2019，49（10）：1227-1281.
2	王金祥，严小龙，潘瑞炽.不定根形成与植物激素的关系［J］.植物生理学通讯，2005，41（2）：133-142.
	Wang J X，Yan X L，Pan R C.Relationship between adventitious root formation and plant hormones［J］.Plant Physiology Communications，2005，41（2）：133-142.
3	熊国胜，李家洋，王永红.植物激素调控研究进展［J］.科学通报，2009，54（18）：2718-2733.
	Xiong G S，Li J Y，Wang Y H.Advances in the regulatiin and crosstalks of phylohomones［J］.Chinese Science Bulletin，2009，54（18）：2718-2733.
4	许智宏，张宪省，苏英华，等.植物细胞全能性和再生［J］.中国科学：生命科学，2019，49（10）：1282-1300.
	Xu Z H，Zhang X S，Su Y H，et al.Plant cell totipotency and regeneration［J］.Scientia Sinica Vitae，2019，49（10）：1282-1300.
5	李朝婵，赵云龙，张冬林，等.长蕊杜鹃扦插内源激素变化及解剖结构观察［J］.林业科学研究，2012，25（3）：360-365.
	Li C C，Zhao Y L，Zhang D L，et al.Changes of endogenous hormones and anatomical structure of Rhododendron stamineum during cutting propagation［J］.Forest Research，2012，25（3）：360-365.
6	赵云龙，陈训，李朝婵.糙叶杜鹃扦插生根过程中生理生化分析［J］.林业科学，2013，49（6）：45-51.
	Zhao Y L，Chen X，Li C C.Dynamic of physiology and biochemistry during wild Rhododendron scabrifolium cutting propagation［J］.Scientia Silvae Sinicae，2013，49（6）：45-51.
7	温绍廷，顾泽玮，喻杰，等.伤口信号调节植物再生的研究进展［J］.植物生理学报，2020，56（8）：1719-1728.
	Wen S T，Gu Z W，Yu J，et al.Research progresses on wound signaling in regulation of plant regeneration［J］.Plant Physiology Journal，2020，56（8）：1719-1728.
8	张帆，王鸿.桃硬枝扦插生根机理研究进展［J］.植物生理学报，2019，55（11）：1595-1606.
	Zhang F，Wang H.Research status of rooting mechanism of peach hardwood cutting［J］.Plant Physiology Journal，2019，55（11）：1595-1606.
9	麻文俊，张守攻，王军辉，等.日本落叶松扦插生根期内源激素和营养物质及酚酸含量变化特征［J］.西北植物学报，2013，33（1）：109-115.
	Ma W J，Zhang S G，Wang J H，et al.Endogenous hormones，nutritive material and phenolic acid variation in cuttings of Japanese larch during rooting［J］.Acta Botanica Boreali-Occidentalia Sinica，2013，33（1）：109-115.
10	袁东升，李月松，周洪义.彩叶植物图鉴［M］.中国林业出版社，2015：52.
	Yuan D S，Li Y S，Zhou H Y.Caiye Zhiwu Tujian［M］.China Forestry Publishing House，2015：52.
11	赵今哲，刘国彬，张鸿翎，等.生长调节剂处理的金塔柏扦插不定根解剖学研究［J］.西南林业大学学报，2019，39（2）：173-178.
	Zhao J Z，Liu G B，Zhang H L，et al.Anatomical study on adventitious root of Platycladus orientalis cuttings treated by growth regulator［J］.Journal of Southwest Forestry University，2019，39（2）：173-178.
12	刘娜娜，侯元凯，蓝正勇.世界柏科彩叶植物种质资源［J］.世界林业研究，2015，28（4）：24-31.
	Liu N N，Hou Y K，Lan Z Y.Germplasm resources of colored-leaf Cupressaceae plants in the world［J］.World Forestry Research，2015，28（4）：24-31.
13	梁荣纳，沈熙环.侧柏不同种源扦插繁殖的研究［J］.北京林业大学学报，1989，11（4）：47-52.
	Liang R N，Shen X H.Study on vegetative propagation by cuttings delivered from 19 provenannces of Platycladus orientalis［J］.Journal of Beijing Forestry University，1989，11（4）：47-52.
14	杜常健，孙佳成，陈炜，等.侧柏古树实生树和嫁接树的扦插生理和解剖特性比较［J］.林业科学，2019，55（9）：41-49.
	Du C J，Sun J C，Chen W，et al.Comparison of physiological and anatomical characteristics between seedlings and graftings derived from old Platycladus orientalis［J］.Scientia Silvae Sinicae，2019，55（9）：41-49.
15	魏黔春，江泽平，刘建锋，等.侧柏古树扦插试验及插穗营养物质变化［J］.南京林业大学学报：自然科学版，2020，44（1）：63-71.
	Wei Q C，Jiang Z P，Liu J F，et al.Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material［J］.Journal of Nanjing Forestry University：Natural Sciences Edition，2020，44（1）：63-71.
16	刘国彬，赵今哲，张玉平，等.侧柏扦插不定根发生模式研究［J］.西北植物学报，2020，40（6）：987-996.
	Liu G B，Zhao J Z，Zhang Y P，et al.Study on the occurrence pattern of adventitious roots from hardwood branches of Platycladus orientalis［J］.Acta Botanica Boreali-Occidentalia Sinica，2020，40（6）：987-996.
17	Pan X Q，Welti R，Wang X M.Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry［J］.Nature Protocols，2010，5（6）：986-992.
18	Ikeuchi M，Iwase A，Rymen B，et al.Wounding triggers callus formation via dynamic hormonal and transcriptional changes［J］.Plant Physiology，2017，175（3）：1158-1174.
19	Zhu M M，Geng S S，Chakravorty D，et al.Metabolomics of red-light-induced stomatal opening in Arabidopsis thaliana：coupling with abscisic acid and jasmonic acid metabolism［J］.The Plant Journal，2020，101（6）：1331-1348.
20	周容涛，刘洋，冷珊珊，等.欧美杂种山杨微扦插不定根发生过程的解剖学研究［J］.西北植物学报，2013，33（11）：2184-2188.
	Zhou R T，Liu Y，Leng S S，et al.Morphological and anatomical features during the formation of adventitious roots of Populus tremula×P.tremuloides by micro-cuttage multiplication［J］.Acta Botanica Boreali-Occidentalia Sinica，2013，33（11）：2184-2188.
21	周祥明，刘玉堂，赵宪争，等.合欢硬枝扦插生根解剖及相关酶活性变化研究［J］.植物研究，2016，36（1）：58-61.
	Zhou X M，Liu Y T，Zhao X Z，et al.Rooting anatomy of hardwood cutting for Albizia julibrissin Duraxx.and activity change of related enzymes during rooting process［J］.Bulletin of Botanical Research，2016，36（1）：58-61.
22	金建邦，祝遵凌，林庆梅.欧洲鹅耳枥扦插生根及解剖特性［J］.西北农林科技大学学报：自然科学版，2015，43（2）：92-98.
	Jin J B，Zhu Z L，Lin Q M.Rooting characteristics and anatomical structure of Carpinus betulus cuttings［J］.Journal of Northwest A&F University：Natural Science Edition，2015，43（2）：92-98.
23	章建红，施娟娟，夏国华，等.薄壳山核桃硬枝扦插及生根机理研究［J］.安徽农业大学学报，2014，41（2）：203-208.
	Zhang J H，Shi J J，Xia G H，et al.Research of lignified cuttings and rooting mechanism of Carya illinoensis［J］.Journal of Anhui Agricultural University，2014，41（2）：203-208.
24	白晓燕，王力荣，王新卫，等.桃砧木组织培养和扦插生根的解剖学观察［J］.果树学报，2015，32（1）：74-78.
	Bai X Y，Wang L R，Wang X W，et al.Microscopic observation on adventitious root development of micropropagation and cutting propagation on peach rootstocks［J］.Journal of Fruit Science，2015，32（1）：74-78.
25	史锋厚，赵瑞，罗帅，等.红缨海棠嫩枝扦插生根解剖学观察［J］.经济林研究，2018，36（3）：94-99.
	Shi F H，Zhao R，Luo S，et al.The anatomical observation on the rooting of the soft-wood cutting of Malus ‘Hongying’［J］.Nonwood Forest Research，2018，36（3）：94-99.
26	郑巧巧，兰思仁，刘雪蝶，等.生长调节剂对‘香妃’含笑扦插生根及相关酶活性的影响［J］.中南林业科技大学学报，2020，40（5）：67-76.
	Zheng Q Q，Lan S R，Liu X D，et al.Effects of growth regulators on rooting and correlative enzyme activities of Michelia figo ‘Xiangfei’ cuttings［J］.Journal of Central South University of Forestry & Technology，2020，40（5）：67-76.
27	欧阳芳群，付国赞，王军辉，等.欧洲云杉扦插生根进程中内源激素和多酚类物质变化［J］.林业科学，2015，51（3）：155-162.
	Ouyang F Q，Fu G Z，Wang J H，et al.Qualitative analysis of endogenesis hormone and polyphenol during rooting of cuttings in Norway spruce（Picea abies）［J］.Scientia Silvae Sinicae，2015，51（3）：155-162.
28	王志敏，曹均，张玉平，等.圆柏品种‘蓝塔’扦插试验的比较［J］.分子植物育种，2020，18（9）：3107-3114.
	Wang Z M，Cao J，Zhang Y P，et al.Comparison of cutting experiment of Sabina chinensis ‘Lanta’［J］.Molecular Plant Breeding，2020，18（9）：3107-3114.
29	王瑞敏，祝凌云，陈颖，等.金叶银杏硬枝扦插繁殖生根过程及其生根机制研究［J］.中南林业科技大学学报，2020，40（5）：28-37.
	Wang R M，Zhu L Y，Chen Y，et al.Study on rooting process and rooting mechanism of hardwood cuttings of Ginkgo biloba ‘Golden-leaf’［J］.Journal of Central South University of Forestry & Technology，2020，40（5）：28-37.
30	Nag S，Saha K，Choudhuri M A.Role of auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rooting［J］.Journal of Plant Growth Regulation，2001，20：182-194.
31	Negishi N，Nakahama K，Urata N，et al.Hormone level analysis on adventitious root formation in Eucalyptus globulus［J］.New Forests，2014，45（4）：577-587.
32	Gutierrez L，Mongelard G，Floková K，et al.Auxin controls Arabidopsis adventitious root initiation by regulating jasmonic acid homeostasis［J］.The Plant Cell，2012，24（6）：2515-2527.
33	Li S B，Huang P，Ding G C，et al.Optimization of hormone combinations for root growth and bud germination in Chinese fir（Cunninghamia lanceolata） clone leaf cuttings［J］.Scientific Reports，2017，7（1）：5046.
34	Hausman J F.Changes in peroxidase activity，auxin level and ethylene production during root formation by poplar shoots raised in vitro［J］.Plant Growth Regulation volume，1993，13（3）：263-268.
35	Yoo B S，Kim J Y，Kim Y C，et al.Analysis of endogenous hormones associated with hardly rooting Rhododendron species［J］.The Journal of the Korean Society of International Agriculture，2008，20（2）：107-112.
36	Shu W B，Zhou H J，Jiang C，et al.The auxin receptor TIR1 homolog（PagFBL 1） regulates adventitious rooting through interactions with Aux/IAA28 in Populus［J］.Plant Biotechnology Journal，2019，17（2）：338-349.
37	Pop T I，Pamfil D，Bellini C.Auxin control in the formation of adventitious roots［J］.Notulae Botanicae Horti Agrobotanici Cluj-Napoca，2011，39（1）：307-316.
38	Pacurar D I，Perrone I，Bellini C.Auxin is a central player in the hormone cross-talks that control adventitious rooting［J］.Physiologia Plantarum，2014，151（1）：83-96.
39	Meng X Y，Wang Z，He S L，et al.Endogenous hormone levels and activities of IAA-modifying enzymes during adventitious rooting of tree peony cuttings and grafted scions［J］.Horticulture，Environment，and Biotechnology，2019，60（2）：187-197.
40	许晓岗，丁俊刚，童丽丽.激素对楸子插穗内源激素含量和可溶性蛋白组成的影响［J］.南京林业大学学报：自然科学版，2009，33（2）：60-64.
	Xu X G，Ding J G，Tong L L.Changes of soluble proteins and content of endohormone in the process of striking roots of Malus prunifolia Borkh［J］.Journal of Nanjing Forestry University：Natural Science Edition，2009，33（2）：60-64.
41	王小玲，赵忠，权金娥.扦插时间对四倍体刺槐嫩枝插穗内源激素和氧化酶的影响［J］.北京林业大学学报，2011，33（6）：102-106.
	Wang X L，Zhao Z，Quan J E.Effects of planting time on endogenous hormones and oxidase in tetraploid Robinia pseudoacacia softwood cuttings［J］.Journal of Beijing Forestry University，2011，33（6）：102-106.
42	Zhang G F，Zhao F，Chen L Q，et al.Jasmonate-mediated wound signalling promotes plant regeneration［J］.Nature Plants，2019，5（5）：491-497.
43	刘昊，宋晓波，周乃富，等.吲哚丁酸对核桃嫩枝扦插生根及内源激素变化的影响［J］.浙江农林大学学报，2017，34（6）：1038-1043.
	Liu H，Song X B，Zhou N F，et al.Adventitious root formation with IBA and endogenous hormones dynamics in walnut soft-cuttings［J］.Journal of Zhejiang A&F University，2017，34（6）：1038-1043.
44	Ikeuchi M，Ogawa Y，Iwase A，et al.Plant regeneration：cellular origins and molecular mechanisms［J］.Development，2016，143（9）：1442-1451.

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[6]	YANG Liu, WANG Le, XIE Zhong-Kui, GUO Zhi-Hong, ZHANG Yu-Bao. Influence of Endogenous IAA and GA₃ on in vitro Lily Seedling Growth [J]. Bulletin of Botanical Research, 2016, 36(5): 760-767.
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