doi:10.7525/j.issn.1673-5102.2022.02.014

Abstract

Abstract:

To explore a sustainable and stable way to obtain birch branches， a multi branch birch by promoting germination was established. One year old birch seedlings were used as experimental materials， and three cutting heights（10， 20 and 30 cm）and four hormone or chemical agents （20 mg·L^-1 2，4-D， 20 mg·L^-1 KT， 20 mg·L^-1 6-BA and 20 mg·L^-1 KMnO₄） were used to promote the sprouting of birch seedlings. The results showed that the germination rate of birch seedlings was the highest， which reached 65.0% after the first cutting（30 d） and the cutting height was 10 cm. Under the treatment of sawdust moisture， the sprouting rate and sprouting yield of the three cutting heights were higher， the sprouting rate was more than 75.0%， and the yield was more than 77.0 g per plant. The content of total triterpenoids in the branches was the highest at 20 cm cutting height（76.43 mg·g^-1）， followed by 10 cm cutting height. Other treatments were not conducive to the accumulation of total triterpenoids. The content of total triterpenoids in leaves was the highest under the treatment of 6-BA （91.16 mg·g^-1）， which was more than 2.68 times of sawdust treatment（30 cm）. The content of total triterpenoids in the branches and leaves of sawdust， KT and KMnO₄ treatments was significantly lower than that of other treatments. 6-BA was beneficial to the accumulation of oleanolic acid in branches and leaves， while other treatments were not conducive to the accumulation of oleanolic acid. Sawdust treatment was beneficial to the secondary sprouting and yield improvement. The sprouting yield of the treatment with 10 cm cutting height reached 126.96 g per plant. The total triterpenoids content in the branches was 97.99 mg·g^-1 when treated with 6-BA at the cutting height of 30 cm. The content of total triterpenoids in leaves was option when the cutting height was 20 cm， reaching 99.22 mg·g^-1， followed by sawdust treatment group. The results provid technical support for sustainable and efficient utilization of birch resources and obtaining triterpenoids.

Key words: Betula platyphylla Suk., germination promotion, secondary metabolism, triterpenoids, hormone

CLC Number:

S792.153

Yuqi Zhang, Xin Su, Zhiqiang You, Jinbo Fu, Yaguang Zhan, Jing Yin. [J]. Bulletin of Botanical Research, 2022, 42(2): 289-298.

Figures/Tables 10

Table 1

Fig.1

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Table 2

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Table 3

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处理 Treatments	腋芽原基 /个 Axillary bud primordium	萌芽数量 /个 Sprout number	萌芽率 Germination rate /%	萌条数量 /个 Shoot number	萌条率 Shoot rate /%	萌条产量（鲜质量） Shoot yield /g
10 cm（ck）	75	30	40.00	21	70.00	32.13
20 cm（ck）	114	38	33.33	26	68.42	29.35
30 cm（ck）	151	43	28.48	24	55.81	34.22
锯末 Sawdust 10 cm	63	27	42.86	21	77.78	61.34
锯末 Sawdust 20 cm	100	38	38.00	29	76.32	77.18
锯末 Sawdust 30 cm	129	56	43.41	42	75.00	45.33
6-BA 10 cm	74	50	67.57	22	44.00	45.93
6-BA 20 cm	106	37	34.91	17	45.95	20.89
6-BA 30 cm	110	49	44.55	12	24.49	12.94
KT 10 cm	68	47	69.12	20	42.55	36.20
KT 20 cm	127	39	30.71	18	46.15	19.59
KT 30 cm	71	39	54.93	17	43.59	19.89
KMnO₄ 10 cm	59	35	59.32	16	45.71	24.92
KMnO₄20 cm	110	45	40.91	21	46.67	23.59
KMnO₄ 30 cm	105	48	45.71	26	54.17	48.58
2，4-D 10 cm	74	37	50.00	17	45.95	22.73
2，4-D 20 cm	126	35	27.78	29	62.86	26.92
2，4-D 30 cm	125	45	36.00	9	20.00	10.56

处理 Treatments	萌条数量 /个 Shoot number	鲜质量 Fresh weight /g
10 cm（ck）	55	35.88
20 cm（ck）	54	46.22
30 cm（ck）	58	34.88
锯末 Sawdust 10 cm	62	126.96
锯末 Sawdust 20 cm	72	59.78
锯末 Sawdust 30 cm	59	58.65
6-BA 10 cm	37	45.77
6-BA 20 cm	38	29.62
6-BA 30 cm	42	28.40
KT 10 cm	42	34.32
KT 20 cm	34	26.42
KT 30 cm	38	37.71
KMnO₄ 10 cm	37	39.15
KMnO₄ 20 cm	54	44.63
KMnO₄ 30 cm	49	51.19
2，4-D 10 cm	33	25.84
2，4-D 20 cm	53	24.75
2，4-D 30 cm	42	36.87

处理 Treatments	萌条数 /个 Shoot number	鲜质量 Fresh weight /g
10 cm（ck）	31	15.37
20 cm（ck）	15	26.30
30 cm（ck）	24	16.74
锯末 Sawdust 10 cm	29	26.66
锯末 Sawdust 20 cm	19	31.33
锯末 Sawdust 30 cm	16	15.53
6-BA 10 cm	26	15.58
6-BA 20 cm	11	17.66
6-BA 30 cm	23	5.22
KT 10 cm	13	13.98
KT 20 cm	8	17.65
KT 30 cm	28	14.49
KMnO₄ 10 cm	16	18.60
KMnO₄ 20 cm	13	17.36
KMnO₄ 30 cm	4	12.11
2，4-D 10 cm	13	18.37
2，4-D 20 cm	27	21.57
2，4-D 30 cm	26	18.56

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