Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (2): 289-298.doi: 10.7525/j.issn.1673-5102.2022.02.014

• Physiology and Ecology • Previous Articles     Next Articles

Yuqi Zhang1, Xin Su1, Zhiqiang You1, Jinbo Fu1, Yaguang Zhan1,2, Jing Yin1,2()   

  1. 1.Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,Northeast Forestry University,Harbin 150040
    2.College of Life Sciences,Northeast Forestry University,Harbin 150040
  • Received:2021-03-11 Online:2022-03-20 Published:2022-02-22
  • Contact: Jing Yin E-mail:yinjing20135@163.com
  • About author:Zhang Yuqi(1994—),male,postgraduate,research direction for plant secondary metabolism regulation.
  • Supported by:
    Innovation project for college students in Northeast Forestry University(201710225190);National Natural Science Foundation of China(31570589);Fundamental Research Fund for the Central Universities(2572020DY22)


To explore a sustainable and stable way to obtain birch branches, a multi branch birch by promoting germination was established. One year old birch seedlings were used as experimental materials, and three cutting heights(10, 20 and 30 cm)and four hormone or chemical agents (20 mg·L-1 2,4-D, 20 mg·L-1 KT, 20 mg·L-1 6-BA and 20 mg·L-1 KMnO4) were used to promote the sprouting of birch seedlings. The results showed that the germination rate of birch seedlings was the highest, which reached 65.0% after the first cutting(30 d) and the cutting height was 10 cm. Under the treatment of sawdust moisture, the sprouting rate and sprouting yield of the three cutting heights were higher, the sprouting rate was more than 75.0%, and the yield was more than 77.0 g per plant. The content of total triterpenoids in the branches was the highest at 20 cm cutting height(76.43 mg·g-1), followed by 10 cm cutting height. Other treatments were not conducive to the accumulation of total triterpenoids. The content of total triterpenoids in leaves was the highest under the treatment of 6-BA (91.16 mg·g-1), which was more than 2.68 times of sawdust treatment(30 cm). The content of total triterpenoids in the branches and leaves of sawdust, KT and KMnO4 treatments was significantly lower than that of other treatments. 6-BA was beneficial to the accumulation of oleanolic acid in branches and leaves, while other treatments were not conducive to the accumulation of oleanolic acid. Sawdust treatment was beneficial to the secondary sprouting and yield improvement. The sprouting yield of the treatment with 10 cm cutting height reached 126.96 g per plant. The total triterpenoids content in the branches was 97.99 mg·g-1 when treated with 6-BA at the cutting height of 30 cm. The content of total triterpenoids in leaves was option when the cutting height was 20 cm, reaching 99.22 mg·g-1, followed by sawdust treatment group. The results provid technical support for sustainable and efficient utilization of birch resources and obtaining triterpenoids.

Key words: Betula platyphylla Suk., germination promotion, secondary metabolism, triterpenoids, hormone

CLC Number: