Dynamic Changes of Endogenous Hormones in Rootstocks and Scions within Nurse Seedling Graft in Camellia oleifera Under Wound

doi:10.7525/j.issn.1673-5102.2021.02.011

Abstract

Abstract:

In order to analyze the physiological response to the surface healing of rootstocks and scions， and to provide the theoretical support for the growth mechanism， the dynamic changes of endogenous hormones in post-traumatic stress response in Camellia oleifera A. under wound were studied. The six-year-old Changlin 18 and Changlin 53 trees were used， and the contents of IAA， ABA， TZR， Zeatin， SA， JA were assayed using HPLC-MS at different wound stages， and the changes of endogenous hormone in different period and relationship between varieties were analyzed. The results showed that the content of IAA， SA and JA decreased with the increasing time of wound in scions of Changlin 18， and the content of TZR and Zeatin increased first and decreased later which they were respectively reached the highest in S10 and S0. The content of ABA rose continually and reached the peak in S10. In scions of Changlin 53， the contents of IAA and JA would decrease after peak； the TZR， Zeatin， SA were gradually increased after minimum. The content of ABA was gradually decreased in S0 after maximum. The contents of endogenous hormones were higher in Changlin18 than in Changlin53 in stem of rootstock between varieties. The endogenous hormones were decreased in Z0 and increased in Z10 in varieties except the contents of ABA and SA in Changlin53 increased first and then decreased. In stem and root of rootstocks， the contents of endogenous hormones were higher in Changlin 18 than in Changlin 53 except JA； The contents of IAA and ABA in stem of rootstocks were higher than roots which other endogenous hormones in root of rootstocks were higher than in stem. There was difference in the ratio of hormone in varieties and part of rootstocks. The ratios of IAA/ABA， IAA/TZR， IAA/Zeatin， IAA/JA， ABA/TZR， ABA/Zeatin， ABA/JA in scions and rootstocks were higher in Changlin 53 than in Changlin 18. The ratio of SA/IAA in scions was higher in Changlin 18 than Changlin 53， and it was higher in Changlin 18 than Changlin 53 in ratios of SA/JA， SA/ABA of scions while it was higher in Changlin 18 in stem of rootstocks. The ratio of TZR/SA， TZR/JA in scions and stem of rootstocks were higher in Changlin 18 than Changlin 53. There was the significantly positive correlation in IAA and JA after wound， and the correlation of IAA and SA， SA and JA had difference between varieties， the significantly positive correlation in Changlin 18 and the significantly or significantly negative correlation in Changlin 53. There was the significantly positive correlation among TZR， Zeatin， SA and the significantly negative correlation among TZR， Zeatin， SA. In stem of rootstocks， there was very significantly positive correlation among hormones in Changlin 18， and there was the significantly positive correlation among TZR， IAA and JA， Zeatin and IAA and JA while there was the significantly negative correlation in TZR and SA， Zeatin and SA. There was significantly negative correlation in SA and JA. In stem and root of rootstocks， there was the difference in correlation of SA and other hormones among varieties， and other hormones in Changlin 18 and Changlin 53 had the same significantly positive or negative correlation. To sum up， it was easy to callus formation in surface of wound in Changlin 18， but it was difficult in Changlin 53 because there was much more high-level hormone of stress-resistant so that it would be possible that affects healing after grafting wound 10 min. The differences in ratio and contents of hormones between rootstocks and scions may be affected by the morphological reconstruction of grafted unions and the growth of scions in later stage.

Key words: Camellia oleifera, nurse seedling graft, scion, rootstock, wound, endogenous hormone

CLC Number:

S603.8

Wei LONG, Xiao-Hua YAO, Le-Yan LÜ. Dynamic Changes of Endogenous Hormones in Rootstocks and Scions within Nurse Seedling Graft in Camellia oleifera Under Wound[J]. Bulletin of Botanical Research, 2021, 41(2): 232-242.

Figures/Tables 8

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品种 Varieties	激素 Hormone	吲哚乙酸 IAA	脱落酸 ABA	反式玉米素 TZR	玉米素 Zeatin	水杨酸 SA	茉莉酸 JA
18号	IAA	1
	ABA	-0.311	1
	TZR	0.700^*	0.339	1
	Zeatin	0.487	-0.300	0.422	1
	SA	0.977^**	-0.426	0.578	0.494	1
	JA	0.943^**	-0.576	0.501	0.571	0.962^**	1
53号	IAA	1
	ABA	-0.763^*	1
	TZR	-0.593	0.098	1
	Zeatin	-0.432	-0.103	0.935^**	1
	SA	-0.787^*	0.409	0.879^**	0.734^*	1
	JA	0.845^**	-0.410	-0.869^**	-0.812^**	-0.889^**	1

品种 Varieties	激素 Hormone	吲哚乙酸IAA	脱落酸 ABA	反式玉米素 TZR	玉米素 Zeatin	水杨酸 SA	茉莉酸 JA
18号	IAA	1
	ABA	0.889^*	1
	TZR	0.922^**	0.981^**	1
	Zeatin	0.928^**	0.921^**	0.934^**	1
	SA	0.914^*	0.980^**	0.999^**	0.934^**	1
	JA	0.999^**	0.889^*	0.927^**	0.937^**	0.919^**	1
53号	IAA	1
	ABA	0.007	1
	TZR	0.856^*	-0.475	1
	Zeatin	0.918^**	-0.212	0.937^**	1
	SA	-0.606	0.548	-0.845^*	-0.839^*	1
	JA	0.731	-0.603	0.973^**	0.900^*	-0.915^*	1

品种 Varieties	激素 Hormone	吲哚乙酸 IAA	脱落酸 ABA	反式玉米素 TZR	玉米素 Zeatin	水杨酸 SA	茉莉酸 JA
18号	IAA	1
	ABA	0.988^**	1
	TZR	-0.965^**	-0.964^**	1
	Zeatin	-0.971^**	-0.995^**	0.959^**	1
	SA	0.979^**	0.995^**	-0.979^**	-0.991^**	1
	JA	-0.975^**	-0.977^**	0.994^**	0.970^**	-0.984^**	1
53号	IAA	1
	ABA	0.985^**	1
	TZR	-0.997^**	-0.984^**	1
	Zeatin	-0.996^**	-0.979^**	0.987^**	1
	SA	-0.980^**	-0.957^**	0.969^**	0.992^**	1
	JA	-0.954^**	-0.906*	0.940^**	0.966^**	0.963^**	1

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