Rapid Propagation of Parathelypteris angustifrons(Miq.) Ching

doi:10.7525/j.issn.1673-5102.2015.06.005

Abstract

Abstract: The rapid propagation system of Huperzia serrata(Thunb.) Trev. associated fern (Parathelypteris angustifrons(Miq.) Ching) was established by culturing its prothallium. MS+1.0 mg·L^-1 NAA was the most suitable medium for prothallium proliferation. After 60 days of growing in this medium, the fresh weight of prothallium weighed twice heavier compared with one in the control medium (MS without hormone). MS without plant hormone was the optimal medium for inducing sporophyte. The shortest induction time was 22 days and the induction rate was 15%. 6-BA and TDZ showed inhibitory effect on the formation of the sporophyte. The best medium for root induction of sporophyte was MS+1.0 mg·L^-1 IAA. Within 60 days, the medium induced a mean root length of 1.72 cm and 13 roots per explant. Sporophyte grew best in water moss by which the survival rate of transplantation reached 100%.

Key words: Parathelypteris angustifrons, prothallium, rapid propagation

CLC Number:

CHEN Xi1,2,3；HE Mei-Na1,2,3；ZHUANG Hui-Hui1,2,3；GUO Li-Zhu1,2,3；ZHOU Pan1,2,3；HE Wei1,2,3；GUO Bin1,2,3；WEI Ya-Hui1,2,3*. Rapid Propagation of Parathelypteris angustifrons(Miq.) Ching[J]. Bulletin of Botanical Research, 2015, 35(6): 819-824.

References

1.Liu Yujing,Wujisguleng W,Long C,et al.Food uses of ferns in China:a review［J］.Acta Societatis Botanicorum Poloniae,2012,81(4):263-270.

2.Mannan M M,Maridass M,Victor B.A review on the potential uses of ferns［J］.Ethnobotanical leaflets,2008,2008(1):281-285.

3.Tomšík P.Ferns and Lycopods-A Potential Treasury of Anticancer Agents but Also a Carcinogenic Hazard［J］.Phytotherapy Research,2013,28(6):798-810.

4.王玲,和兆荣.药用蕨类植物化学成分研究进展［J］.中国野生植物资源,2006,25(3):1-4.

5.王玛丽,刘红梅,陆树刚,等.岩蕨科、球盖蕨科的系统位置:叶绿体DNAtrnL-F区序列证据［J］.植物研究,2008,28(2):175-178.

6.张丽君,陈洁,王艇.蕨类植物叶绿体rps4基因的适应性进化分析［J］.植物研究,2010,30(1):42-50.

7.梅笑漫,朱圣潮,徐双喜,等.浙江省凤阳山自然保护区蕨类植物区系的研究［J］.植物研究,2005,25(1):99-105.

8.李玉泉,何平,邓洪平,等.重庆地区蕨类植物区系的初步研究［J］.植物研究,2005,25(2):230-235.

9.吴世福.武陵山区蕨类植物研究［J］.植物研究,1998,18(3):291-303.

10.肖宜安,何平,李晓红,等.井冈山自然保护区蕨类植物资源［J］.植物研究,2004,24(1):35-40.

11.张光富,陈瑞冰,钱士心.安徽祁门地区蕨类植物区系研究［J］.植物研究,2005,25(4):488-494.

12.张文霞,钟希文.金星蕨科药用植物化学成分与药理作用研究进展［J］.中国药房,2011,22(27):2582-2584.

13.Fu W,Chen J,Cai Y.Antioxidant,free radical scavenging,antiinflammatory and hepatoprotective potential of the extract from Parathelypteris nipponica(Franch.et Sav.) Ching［J］.Journal of ethnopharmacology,2010,130(3):521-528.

14.Ebihara A,Fraser-Jenkins C R,Parris B S,et al.Rare and threatened pteridophytes of Asia 1.An enumeration of narrowly distributed taxa［J］.Bulletin of the National Museum of Nature and Science,Series B,2012,38(3):93-119.

15.Fernández H,Revilla M A.In vitro culture of ornamental ferns［J］.Plant cell,tissue and organ culture,2003,73(1):1-13.

16.熊国胜,李家洋,王永红.植物激素调控研究进展［J］.科学通报,2009,54(18):2718-2733.

17.孟林,肖阔,赵茂林,等.马蔺组织培养快繁技术体系研究［J］.植物研究,2009,29(02):193-197.

18.李静,夏桂春,龚慧,等.狭眼凤尾蕨的形态发生及组织培养［J］.热带作物学报,2008,29(05):626-631.

19.张敏.薇菜组织培养技术［J］.北方园艺,2008(02):206-208.

20.王辉,秦建蓉,植爽,等.桫椤原叶体增殖及幼孢子体形成试验［J］.林业科技开发,2013,27(05):86-88.

21.吴华,袁丽萍,王洋,等.扇叶铁线蕨孢子无菌繁殖技术研究［J］.园艺学报,2010,37(3):457-464.

22.罗顺元,王任翔.假鞭叶铁线蕨孢子的组织培养［J］.植物生理学通讯,2007,43(1):131-132.

23.Loescher W H,Albrecht C N.Development in vitro of Nephrolepis exaltala cv.Bostoniensis runner tissues［J］.Physiological Plant,1979,47(4):250-254.

24.Suzanne M,Rogers D,Banister S.Micropropagation of Notholaena ‘Sun-Tuff’ fern［J］.HortScience,1997,27(1):1224-1225.

25.吕亚丽,郭顺星,孟志霞.天山雪莲组培苗生根培养体系的优化［J］.中国药学杂志,2009,44(17):1291-1294.

26.张婷婷,王晓倩,高凤,等.球子蕨孢子的无菌繁殖［J］.植物生理学报,2012,48(11):1079-1083.

27.郭捡,刘婷婷,孟宪利,等.鹿角蕨的孢子培养及其繁殖［J］.园艺学报,2013,40(001):155-162.

28.王晓倩,张婷婷,孟卓,等.阔鳞鳞毛蕨孢子的无菌繁殖［J］.植物生理学报,2014,50(2):159-163.

29.黄少锋,洪生标.水苔在兰花生产中的应用及合理开发［J］.中国花卉盆景,2005(8):21-21.

30.李杨,余蓉培,李慧,等.观赏蕨类植物组织培养研究进展［J］.园艺学报,2012,39(9):1839-1848.

[1]	ZHOU Fang, XU Jian-Qiu, AN Juan-Yan, JIANG Hong-Mao, WANG Zhen-Xi, ZHANG Tian-Xing, LI Kai-Long. Tissue Culture and Rapid Propagation of Trollius ledebouri [J]. Bulletin of Botanical Research, 2018, 38(4): 619-624.
[2]	CHEN Suo-Liang;LV Xiu-Li;CUI Xin-Hong;WANG Cheng;ZHANG Qun;ZHU Yi;LU Liang. Tissue Culture of Gunnera manlcata* L. [J]. Bulletin of Botanical Research, 2014, 34(3): 328-332.
[3]	GONG Xue-Yuan;DU Ya-Tian;ZHANG Xiang-Yu;LIU Jiao. Rapid Propagation Technique System of Blueberry* [J]. Bulletin of Botanical Research, 2014, 34(1): 131-135.
[4]	MAO Yan-Ping;SU Zhi-Xian;ZOU Li-Juan;WU Qing-Gui. Tissue Culture and Rapid Propagation of Rare Plant in Codariocalyx motorius*(Houtt.) [J]. Bulletin of Botanical Research, 2010, 30(1): 106-110.
[5]	MA Ying;LI Kai-Long;TIAN Xin-Hua;LI Jing. Culture in Vitro and Rapid Propagation System with Potentilla fruiticosa ‘Gold Drop’ [J]. Bulletin of Botanical Research, 2009, 29(5): 623-627.
[6]	CHU Li-Min;SUN Zhou-Ping. Tissue Culture and Rapid Propagation of Acanthopanax senticosus* [J]. Bulletin of Botanical Research, 2009, 29(4): 505-508.
[7]	MENG Lin;XIAO Kuo;ZHAO Mao-Lin;ZHANG Guo-Fang. Technological System of Tissue Culture and Rapid Propagation of Iris lactea Pall. var. chinensis(Fisch.) Koidz. [J]. Bulletin of Botanical Research, 2009, 29(2): 193-197.
[8]	WANG Wen-Wen;MA Qiu-Yue;ZHU Jun-Yi;GU Di-Zhou. In Vitro Rapid Propagation System and in Vitro Germplasm Peservation of Rhododendron schlippenbachii* Maxim. [J]. , 2009, 29(2): 198-203.
[9]	ZHANG Yu-Hong;QU Wei-Di. Different Culture Conditions on Rapid Propagation of Phellodendron amurense [J]. Bulletin of Botanical Research, 2008, 28(2): 236-239.
[10]	GUO Yan-Ru;ZHAN Ya-Guang;JI Li-Li. The Rapid Propagation Technology of Sorbus pohuashanensis* by Axillary Bud Regeneration Way [J]. Bulletin of Botanical Research, 2007, 27(2): 224-228.
[11]	LIN Ping, PU Xiao-Lan. RAPID PROPAGATION OF PHOTINIA GLOMERATA IN VITRO [J]. Bulletin of Botanical Research, 2003, 23(3): 280-284.