Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2007, Vol. 27 ›› Issue (2): 224-228.doi: 10.7525/j.issn.1673-5102.2007.02.021

Previous Articles     Next Articles

The Rapid Propagation Technology of Sorbus pohuashanensis by Axillary Bud Regeneration Way

GUO Yan-Ru;ZHAN Ya-Guang*;JI Li-Li   

  1. 1.Department of Life Science,Yuncheng University,Yuncheng 044000 2.College of Life Science, Northeast Forestry University,Harbin 1500403.Heilongjiang Forestry Vocation-Technical College,Mudanjiang 157011
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-03-20 Published:2007-03-20
  • Contact: ZHAN Ya-Guang
  • Supported by:

Abstract: In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA(2.5 mg·L-1) and 2.4-D(0.5 mg·L-1); the optimum medium for bud proliferation was MS+6-BA(1 600~2 100)mg·L-1+NAA(0.140~0.230)mg·L-1, 13 shoots per explant could be obtained at average;the shoots grew strongly on MS+6-BA0.2 mg·L-1+IBA0.2 mg·L-1; And 1/2MS+IBA0.3 mg·L-1 was the best medium for shoot rooting,with the rooting rate at 90.20%,and the average root number of 5.96;Plantlets survival rate reached 95.55% when transplanted on the mixture(humus 3:peat soil 2:sand 1).

Key words: Sorbus pohuashanensis axillary bud, tissue culture, rapid propagation

CLC Number: