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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (4): 619-624.doi: 10.7525/j.issn.1673-5102.2018.04.018

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Tissue Culture and Rapid Propagation of Trollius ledebouri

ZHOU Fang, XU Jian-Qiu, AN Juan-Yan, JIANG Hong-Mao, WANG Zhen-Xi, ZHANG Tian-Xing, LI Kai-Long   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2017-12-19 Online:2018-07-15 Published:2018-07-21
  • Supported by:
    The National Undergraduate Innovation Training Program of China(201620225015)

Abstract: The seeds of Trollius ledebouri Reichb. were used by different concentration and treating time of GA3 to promote germination, and their buds were inoculated on different types of medium with different hormone. A rapid propagation system by tissue culture was established and become basis for large-scale production of T.ledebouri. The results showed that:(1)the seeds of T.ledebouri Reichb was efficiently sterilized by 0.1% HgCl2 for 20 min after soaking in 600 mg·L-1 GA3 solution for 48 h and the germination was high; (2)the suitable medium for multiplication of T.ledebouri was MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 NAA; (3)the suitable rooting medium was MS without any hormone.

Key words: Trollius ledebouri Reichb., tisssue culture, rapid propagation

CLC Number: