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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (5): 623-627.doi: 10.7525/j.issn.1673-5102.2009.05.021

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Culture in Vitro and Rapid Propagation System with Potentilla fruiticosa ‘Gold Drop’

MA Ying;LI Kai-Long;TIAN Xin-Hua;LI Jing   

  1. (1.Northeast forestry university,Haerbin150040) (2.Heilongjiang Academy of Forestry Institute,Haerbin150081)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: LI Kai-Long
  • Supported by:

Abstract: The buds of Potentilla fruiticosa ‘Gold Drop’ was chosen the adapted primarily culture explants by experiment. The best medium was screened for axillary budprimarily culture, bud seedling subculture multiplication and tissue culture shoot rooting by L9(34) orthogonal experiment. The rapid propagation system of P.fruiticosa ‘Gold Drop’, axillary bud induction-subculture multiplication-rooting culture, was formed. The result showed The differentiation medium of axillary bud induction was MS+6-BA 2 mg·L-1+NAA 0.1 mg·L-1. MS, 6-BA and NAA was the main factors and MS>6-BA>NAA. The induction rate was reached to 96.33%. 6-BA was the principal hormone that affects the proliferation of P.fruiticosa buds which showed a significant discrepancy(p=0.000 1), and the reproduction effect was the best when the concentration was 2.0 mg·L-1. MS+6-BA 2.0 mg·L-1+NAA 0.6 mg·L-1+KT 0.2 mg·L-1 were defined as the optimum mediums with the multiples of proliferation in 15 days reaching 6.12. KT and NAA were the main factors on affecting rooting by variance analysis and IBA was no influencing. So KT and NAA were chosen for inducing hormone.The optimal rooting medium was MS+NAA 0.1 mg·L-1+KT 1.0 mg·L-1 by multiple comparison.The rooting number was 8.63 and rooting rate reached to 95.33%.

Key words: Potentilla fruiticosa &lsquo, Gold Drop&rsquo, In vitro culture, orthogonal test, rapid propagation

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