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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (2): 193-197.doi: 10.7525/j.issn.1673-5102.2009.02.012

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Technological System of Tissue Culture and Rapid Propagation of Iris lactea Pall. var. chinensis(Fisch.) Koidz.

MENG Lin;XIAO Kuo;ZHAO Mao-Lin;ZHANG Guo-Fang   

  1. (1.Beijing Research and Development Center for Grasses and Environment,Beijing 100097) (2.CCTV.com,Beijing 100036) (3.Beijing Agribiotechnology Research Center,Beijing 100097)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
  • Contact: MENG Lin
  • Supported by:

Abstract: Iris lactea Pall. var. chinensis(Fisch.) Kordz. is a kind of water-saving, ornamental, medicinal and fodder plants with excellent characteristics. In this paper, the mature seeds were used as the explants, and the combining medium schemes for inducing callus, differentiation and rooting, etc. were designed with different concentration of 2,4-D, NAA, BA, KT based on the MS culture medium, and the system of tissue culture and rapid propagation of I. lactea was studied. The results showed that the variable ratio of hormones had the great effects on the rates of callus, and the mediums of MS+2,4-D 4 mg·L-1+BA2 mg·L-1、MS+2,4-D 4 mg·L-1+BA5 mg·L-1 and MS+2,4-D 2 mg·L-1+KT1.5 mg·L-1 could give higher rates of callus (more than 58%) than others, so they were thought as the greatest combining schemes. MS+BA4 mg·L-1 and MS+BA1 mg·L-1+NAA0.15 mg·L-1 were the optimal schemes for the differentiation of callus. The optimal regeneration and enrichment culture mediums were MS+BA2 mg·L-1+NAA0.1 mg·L-1 and MS+BA1 mg·L-1+NAA0.2 mg·L-1. The best medium for rooting was 1/2MS, and the schemes of 1/2MS+IBA0.5 mg·L-1, 1/2MS+IBA 0.5 mg·L-1+NAA0.5 mg·L-1 could also have good effects on the rootgrowth. The rooting plantlets were easily planted on the mixed medium of Vsoil∶Vsand=2∶1 without any training. Therefore, the available system of tissue culture and rapid propagation for I. lactea was established including the mature embryo-inducing of callus-differentiation and regeneration of plantlets-propagation-rootingtransplanting.

Key words: Iris lactea Pall. var. chinensis(Fisch.) Kordz., tissue culture, rapid propagation

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