Abstract: We used the ripe capsules from wild Bletilla striata as explants to establish regeneration system. The optimal medium for seed germination was 1/2MS+6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1. The most suitable medium for PLB multiplication was MS+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1, while MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1 was appropriate for PLB differentiation. The optimal medium for adventitious buds multiplication was MS+6-BA 0.5 mg·L^-1+KT 1.0 mg·L^-1+NAA 0.1 mg·L^-1+AC 0.5 g·L^-1+potato juice 150 g·L^-1, and the proliferation rate was up to 343% after 30 days. The most suitable rooting medium was 1/2MS+IBA 0.5 mg·L^-1+AC 0.5 g·L^-1+potato juice 100 g·L^-1 with the rooting rate of 100%. The best way of tube seedlings transplanting was under the volume ratio of sphagna: batk: Coconut chaff: wood flour of 2∶2∶1∶1 with 99% of survival rate.

Key words: Bletilla striata, regeneration system, optimization