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Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (6): 819-824.doi: 10.7525/j.issn.1673-5102.2015.06.005

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Rapid Propagation of Parathelypteris angustifrons(Miq.) Ching

CHEN Xi1,2,3;HE Mei-Na1,2,3;ZHUANG Hui-Hui1,2,3;GUO Li-Zhu1,2,3;ZHOU Pan1,2,3;HE Wei1,2,3;GUO Bin1,2,3;WEI Ya-Hui1,2,3*   

  1. 1.Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,Xi’an 710069;
    2.Shanxi Provincial Key Laboratory of Biotechnology,Xi’an 710069;
    3.College of Life Science,Northwest University,Xi’an 710069
  • Online:2015-11-20 Published:2016-01-18
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Abstract: The rapid propagation system of Huperzia serrata(Thunb.) Trev. associated fern (Parathelypteris angustifrons(Miq.) Ching) was established by culturing its prothallium. MS+1.0 mg·L-1 NAA was the most suitable medium for prothallium proliferation. After 60 days of growing in this medium, the fresh weight of prothallium weighed twice heavier compared with one in the control medium (MS without hormone). MS without plant hormone was the optimal medium for inducing sporophyte. The shortest induction time was 22 days and the induction rate was 15%. 6-BA and TDZ showed inhibitory effect on the formation of the sporophyte. The best medium for root induction of sporophyte was MS+1.0 mg·L-1 IAA. Within 60 days, the medium induced a mean root length of 1.72 cm and 13 roots per explant. Sporophyte grew best in water moss by which the survival rate of transplantation reached 100%.

Key words: Parathelypteris angustifrons, prothallium, rapid propagation

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