Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (6): 886-896.doi: 10.7525/j.issn.1673-5102.2020.06.011
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Li-Qiang ZHAO1,2, Chun-Miao SHAN1,2, Sheng-Xiang ZHANG1,2, Yuan-Yuan SHI1,2, Ke-Long MA3, Jia-Wen WU2,4,5()
Received:
2019-11-19
Online:
2020-11-20
Published:
2020-11-04
Contact:
Jia-Wen WU
E-mail:wujiawen@ahtcm.edu.cn
About author:
ZHAO Li-Qiang(1995—),male,master degree candidate,mainly engaged in transcriptomics research.
Supported by:
CLC Number:
Li-Qiang ZHAO, Chun-Miao SHAN, Sheng-Xiang ZHANG, Yuan-Yuan SHI, Ke-Long MA, Jia-Wen WU. Identification of Key Enzyme Genes Involved in Anthocyanin Synthesis pathway in Clinopodium gracile by Transcriptome Analysis[J]. Bulletin of Botanical Research, 2020, 40(6): 886-896.
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URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2020.06.011
Table 1
Number of unigenes involved in secondary metabolism pathways in C.gracile
其他次生代谢产物的生物合成途径 Biosynthesis of other secondary metabolites pathway | 通路ID Pathway ID | 基因数目 Unigene number |
---|---|---|
咖啡因代谢Caffeine metabolism | ko00232 | 16 |
内酰胺生物合成Monobactam biosynthesis | ko00261 | 92 |
苯并嗪生物合成Benzoxazinoid biosynthesis | ko00402 | 36 |
吲哚生物碱生物合成Indole alkaloid biosynthesis | ko00901 | 187 |
苯丙素生物合成Phenylpropanoid biosynthesis | ko00940 | 1 406 |
黄酮类生物合成Flavonoid biosynthesis | ko00941 | 455 |
花青素生物合成Anthocyanin biosynthesis | ko00942 | 72 |
异黄酮生物合成Isoflavonoid biosynthesis | ko00943 | 89 |
黄酮和黄酮醇生物合成 Flavone and flavonol biosynthesis | ko00944 | 60 |
芪类,二芳基庚烷和姜辣素生物合成 Stilbenoid, diarylheptanoid, gingerol biosynthesis | ko00945 | 181 |
异喹啉生物碱生物合成 Isoquinoline alkaloid biosynthesis | ko00950 | 177 |
丙烷、哌啶、吡啶生物碱合成 Tropane, piperidine,pyridinealkaloid biosynthesis | ko00960 | 142 |
甜菜红碱生物合成Betalain biosynthesis | ko00965 | 26 |
芥子油苷生物合成Glucosinolate biosynthesis | ko00966 | 110 |
Table 2
Key enzymes for the anthocyanin
关键酶缩写 Abbreviation | 名称 Key enzymes | EC编号 EC number | 基因数目 Unigene number |
---|---|---|---|
PAL | 苯丙氨酸氨裂解酶 phenylalanine ammonia-lyase | 4.3.1.24 | 7 |
4CL | 4-香豆素辅酶a连接酶 4-coumarate—CoA ligase | 6.2.1.12 | 10 |
DFR | 双功能二氢黄酮醇4-还原酶 bifunctional dihydroflavonol 4-reductase | 1.1.1219 | 7 |
CHS | 查耳酮合酶chalcone synthase | 2.3.1.74 | 5 |
CHI | 查耳酮异构酶chalcone isomerase | 5.5.1.6 | 4 |
F3H | 黄烷酮3-双加氧酶flavanone 3-dioxygenase | 1.14.11.9 | 7 |
Fig.7
The secondary structure and tertiary structure of DFR in C.gracileA.The tertiary structure of DFR in C.gracile with helices colored in red,strands colored in yellow; B.The purple region shows the binding site for NAD+;C.The secondary structure of DFR in C.gracile(The black underline shows the NAD+ binding site)
Table 3
Homologous alignment of DFR protein
登录号 GenBank ID | 功能蛋白归类 Classification of protein functional | 物种来源 Source | 同源性 Homology(%) | 一致性 Identity(%) |
---|---|---|---|---|
PPS19807.1 | DFR | 海岛棉Gossypium barbadense | 52 | 69 |
PIN26544.1 | DFR | 哥伦比亚锦葵 Herrania umbratica | 65 | 76 |
XP017435247.1 | DFR | 赤豆Vigna angularis | 52 | 69 |
XP027115391.1 | DFR | 咖啡树Coffea arabica | 55 | 69 |
XP017189793.2 | DFR | 苹果Malus domestica | 54 | 70 |
XP017614626.1 | DFR | 木本棉Gossypium arboreum | 53 | 69 |
XP010053799.1 | DFR | 巨桉Eucalyptus grandis | 52 | 68 |
XP020411735.1 | DFR | 桃Prunus persica | 55 | 69 |
XP021808031.1 | DFR | 甜樱桃Prunus avium | 54 | 69 |
XP019419143.1 | DFR | 狭叶羽扇豆Lupinus angustifolius | 53 | 68 |
XP003554760.1 | DFR | 大豆Glycine max | 50 | 66 |
RVX09916.1 | DFR | 葡萄Vitis vinifera | 51 | 67 |
XP019191170.1 | DFR | 牵牛花Ipomoea nil | 53 | 67 |
RAL54198.1 | DFR | 菟丝子Cuscuta australis | 60 | 75 |
PIN26544.1 | DFR | 风铃木Handroanthus impetiginosus | 65 | 76 |
XP011093542.1 | DFR | 芝麻Sesamum indicum | 65 | 77 |
TEY70549.1 | DFR | 一串红Salvia splendens | 70 | 79 |
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