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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (5): 794-800.doi: 10.7525/j.issn.1673-5102.2023.05.016

• Plant reproductive biology • Previous Articles    

Observation on the Formation and Composition Analysis of Blue Pollen of Caryopterisincana

Qinghua YANG1,2, Binjie GE3, Dasheng ZHANG4()   

  1. 1.College of Biological Environment,Nanjing Forestry University,Nanjing Forestry University,Nanjing 210037
    2.Shanghai Expo Culture Park Construction Management Co. ,Ltd. ,Shanghai 200126
    3.Eastern China Conservation Centre for Wild Endangered Plant Resources,Shanghai Chenshan Botanical Garden,Shanghai 201602
    4.Shanghai Chenshan Scientific Research Center of CAS Center for Excellence in Molecular Plant Sciences,Shanghai Chenshan Botanical Garden,Shanghai 201602
  • Received:2023-03-07 Online:2023-09-20 Published:2023-09-05
  • Contact: Dasheng ZHANG E-mail:dszhang@cemps.ac.cn
  • About author:YANG Qinghua(1980—),male,senior engineer,engaged in plant resources and conservation research.
  • Supported by:
    National Natural Science Foundation of China(32072620);National Natural Science Foundation of Shanghai(22ZR1422400);Special Fund for Scientific Research of Shanghai Landscaping & City Appearance Administrative Bureau(G202405)


In majority of plants, colorful pollen is rarely seen on the surface of pollen except light yellow. In this article, the existence of blue pollen was discovered in Caryopteris incana, and the color of anther and pollen at different developmental stages was observed by microscope, and the anthocyanin components of mature pollen were analyzed by UPLC/MS(Ultra Performance Liquid Chromatography-tandem mass spectrometry). The results showed that mature anthers and pollens were blue due to the rich anthocyanins. The color of anther and pollen changed obviously at young buds, early flowering buds and flowering stages, which were light yellow, light blue and blue purple respectively. The blue pigments of pollen mainly existed in the surface of pollen coat and tapetal cells by the cross-section observation. The UPLC/MS analysis showed that there were six kinds of anthocyanins on the pollen surface at the flowering stage, among which the main blue components were Delphinidin 3-O-glucoside and Malvidin 3,5-O-diglucoside, followed by Peonidin, Cyanidin and Petunidin, while the content of Pelargonidin was the lowest.

Key words: Caryopteris incana, anther, pollen, anthocyanin

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