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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (4): 550-561.doi: 10.7525/j.issn.1673-5102.2023.04.008

• Physiology and Ecology • Previous Articles     Next Articles

Changes and Influencing Factors of Anthocyanins in Hibiscus syriacus During Flowering

Zhezhe LI1,2, Yidan ZHANG1,2, Bo WANG3, Zhenghao WANG4, Lu WANG1,2, Beibei CHENG1,2()   

  1. 1.Hebei Normal University of Science and Technology,Qinhuangdao 066000
    2.Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,Qinhuangdao 066000
    3.Tai’an Shidai Garden Technology Development Co. ,Ltd. ,Tai’an 271000
    4.Culaishan Forest Farm of Tai’an City,Tai’an 271000
  • Received:2023-03-22 Online:2023-07-20 Published:2023-07-03
  • Contact: Beibei CHENG E-mail:beibei87613@126.com
  • About author:LI Zhezhe(1995—),male,master student,mainly engaged in research on plant physiology and ecology.
  • Supported by:
    General Survey of Herbaceous Plant Germplasm Resources in Shandong Province(lcyz〔2021〕 No.1);Hebei University Horticultural Crop Breeding Application Technology Research and Development Center Project(YF201404);Hebei Provincial Professional Degree Graduate Training Practice Base(Tangshan botanical garden,202018);Germplasm Resource Bank of State Forestry and Grassland Administration(2005DKA210003);Innovation and Entrepreneurship Training Program for College Students(S202210798050)


In order to explore the law of flower color change of Hibiscussyriacus during the flowering process and the influence of physical and chemical factors such as pH, metal ions and water content on its flower color, the composition and physicochemical factors of anthocyanins in the petals of H. syriacus ‘Qiansiban’ and ‘White Chiffon’ at different blossoming periods were studied respectively by the color chromato-meter, ion emission spectrometer and high performance liquid chromatography-mass spectrometry. The results showed that: (1)In terms of the composition of anthocyanins, 10 kinds of flavonoids such as Quercetin-3-glucoside, Luteolin-7-O-glucoside, and Santolol-7-O-glucoside were detected and identified in the petals of the two varieties, and 5 kinds of anthocyanins such as Mallorin-3-O-glucoside were detected and identified in the H. syriacus ‘Qiansiban’, and no anthocyanins were detected in the H. syriacus ‘White Chiffon’. (2)In the process of blossoming, the brightness of the flower color increased, the color degree decreased, and the color changed from purplish red to lavender, and light yellow to white respectively. During the blossoming, the composition of anthocyanins remained unchanged, and the content of anthocyanins and total anthocyanins gradually decreased in H. syriacus ‘Qiansiban’. (3)The pH of petals in H. syriacus ‘Qiansiban’ and ‘White Chiffon’ were both acidic, and the activities of Peroxidase(POD) and Polyphenol oxidase(PPO) showed significant difference in different periods. Among them, POD activity in H. syriacus ‘Qiansiban’ was higher than that in H. syriacus ‘White Chiffon’, while PPO activity was the opposite; calcium and magnesium ions were the highest content of metal ions in H. syriacus petals, while copper, iron, manganese, zinc and other metal ions had low content in H. syriacus petals. (4)In terms of correlation analysis, during the blossoming of H. syriacus ‘Qiansiban’, the color degree C* was significantly positively correlated with hue a*, and significantly positively correlated with calcium ion, iron ion and manganese ion respectively; during the blossoming of H. syriacus ‘White Chiffon’, the color degree C* was significantly positively correlated with hue b* and PPO activity, and negatively correlated with hue a* respectively. The research showed that the anthocyanins were the material basis of flower color change of H. syriacus flower petals. Besides anthocyanin content, the flower color change process was also closely related to the content of calcium, iron, manganese and other metal ions. PPO activity was a potential biochemical factor to promote the degradation of flavonoids in white flower varieties.

Key words: Hibiscus syriacus, flower color, anthocyanins, flavonoids, influence factor

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